1887

Abstract

Gram-stain-negative, aerobic, halophilic bacteria, designated SCM-1, LCM10-1 and CTBL-B-147, were isolated from modified half-strength SWM-III medium, PES medium and thalli after laboratory cultivation of a red alga, . Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolates were affiliated to the genus of the class , and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, CH-B427, was 98.8 %. The DNA G+C contents of the new isolates were in the range of 61.4–62.3 mol%. DNA–DNA relatedness values of strain SCM-1 with other type strains of the genus were less than 15.9 %. The new isolates contained Q-10 as the predominant ubiquinone, phosphatidylcholine, phosphatidylglycerol, an unidentified amino lipid and an unidentified lipid as the main polar lipids, and Cω7, Cω7 and C as the major fatty acids (>10 % of the total). Strain SCM-1 could be differentiated from JCM 21792 by 35 morphological and phenotypic characteristics. On the basis of the phylogenetic, genetic and phenotypic properties of the new isolates, the name sp. nov. is proposed, with strain SCM-1 ( = LMG 27110 = NBRC 109054) as the type strain.

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science for Young Scientists
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.053090-0
2014-02-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/438.html?itemId=/content/journal/ijsem/10.1099/ijs.0.053090-0&mimeType=html&fmt=ahah

References

  1. Akagawa-Matsushita M., Itoh T., Katayama Y., Kuraishi H., Yamasato K. ( 1992 ). Isoprenoid quinone composition of some marine Alteromonas, Marinomonas, Deleya, Pseudomonas and Shewanella species. . J Gen Microbiol 138, 22752281. [View Article]
    [Google Scholar]
  2. Alain K., Tindall B. J., Intertaglia L., Catala P., Lebaron P. ( 2008 ). Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea. . Int J Syst Evol Microbiol 58, 25112519. [View Article] [PubMed]
    [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  4. Baumann L., Baumann P., Mandel M., Allen R. D. ( 1972 ). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110, 402429.[PubMed]
    [Google Scholar]
  5. Biebl H., Allgaier M., Tindall B. J., Koblizek M., Lünsdorf H., Pukall R., Wagner-Döbler I. ( 2005 ). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. . Int J Syst Evol Microbiol 55, 10891096. [View Article] [PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  7. Ikemoto S., Katoh K., Komagata K. ( 1978 ). Cellular fatty acid composition in methanol-utilizing bacteria. . J Gen Appl Microbiol 24, 4149. [View Article]
    [Google Scholar]
  8. Ivanova E. P., Gorshkova N. M., Sawabe T., Zhukova N. V., Hayashi K., Kurilenko V. V., Alexeeva Y., Buljan V., Nicolau D. V. & other authors ( 2004 ). Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina). . Int J Syst Evol Microbiol 54, 475480. [View Article] [PubMed]
    [Google Scholar]
  9. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  10. Komagata K., Suzuki K. ( 1988 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  11. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P. ( 2000 ). Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., alpha-3-Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho Lake. . Int J Syst Evol Microbiol 50, 303313. [View Article] [PubMed]
    [Google Scholar]
  12. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . New York:: Wiley;.
    [Google Scholar]
  13. Luthria D. L., Sprecher H. ( 1993 ). 2-Alkenyl-4,4-dimethyloxazolines as derivatives for the structural elucidation of isomeric unsaturated fatty acids. . Lipids 28, 561564. [View Article] [PubMed]
    [Google Scholar]
  14. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  15. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  16. Ogata E. ( 1970 ). On a new algal culture medium SWM‐III. . Bull Jpn Soc Phycol 18, 171173 (in Japanese).
    [Google Scholar]
  17. Provasoli L. ( 1968 ). Media and prospects for the cultivation of marine algae. . In Cultures and Collections of Algae, pp. 6375. Edited by Watanabe A., Hattori A. . Tokyo:: Japanese Society of Plant Physiogists;.
    [Google Scholar]
  18. Pukall R., Buntefuß D., Frühling A., Rohde M., Kroppenstedt R. M., Burghardt J., Lebaron P., Bernard L., Stackebrandt E. ( 1999 ). Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria. . Int J Syst Bacteriol 49, 513519. [View Article] [PubMed]
    [Google Scholar]
  19. Satomi M., Kimura B., Mizoi M., Sato T., Fujii T. ( 1997 ). Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. . Int J Syst Bacteriol 47, 832836. [View Article] [PubMed]
    [Google Scholar]
  20. Sawabe T., Oda Y., Shiomi Y., Ezura Y. ( 1995 ). Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. . Microb Ecol 30, 193202. [View Article] [PubMed]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  22. Sorokin D. Y. ( 1995 ). Sulfitobacter pontiacus gen. nov., sp. nov. – a new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. . Microbiology (English translation of Mikrobiologiia) 64, 295305.
    [Google Scholar]
  23. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.053090-0
Loading
/content/journal/ijsem/10.1099/ijs.0.053090-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error