1887

Abstract

16S rRNA gene sequence analysis of eight strains (BR 3299, BR 3296, BR 10192, BR 10193, BR 10194, BR 10195, BR 10196 and BR 10197) isolated from nodules of cowpea collected from a semi-arid region of Brazil showed 97 % similarity to sequences of recently described rhizobial species of the genus . Phylogenetic analyses of four housekeeping genes (, , and ), DNA–DNA relatedness and AFLP further indicated that these strains belong to a novel species within the genus . Our data support the hypothesis that genes related to nitrogen fixation were obtained via horizontal gene transfer, as sequences of genes were very similar to those found in members of the genera and , which are not immediate relatives of the genus , as shown by 16S rRNA gene sequence analysis. Phenotypic traits, such as host range and carbon utilization, differentiate the novel strains from the most closely related species, , and . Therefore, these symbiotic nitrogen-fixing bacteria are proposed to be representatives of a novel species, for which the name sp. nov. is suggested. The type strain is BR3299 ( = HAMBI 3457).

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2014-03-01
2019-09-20
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