1887

Abstract

A novel desiccation-tolerant, xeroprotectant-producing bacterium, designated strain 4J27, was isolated from a rhizosphere subjected to seasonal drought in Granada, Spain. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate within the genus , its closest relative being Shep3 DSM 18606, with which it showed 99.23 % 16S rRNA gene sequence similarity. DNA–DNA hybridization measurements showed less than 25 % relatedness between strain 4J27 and DSM 18606. The DNA base composition of strain 4J27 was 65.3 mol%. The main fatty acids were anteiso C, anteiso C, C and iso C and the major menaquinone was MK-9 (H). The peptidoglycan type was A3α with an -Lys–-Ser–-Thr–-Ala interpeptide bridge. The bacterium tested positive for catalase activity and negative for oxidase activity. Phylogenetic, chemotaxonomic and phenotypic analyses indicated that the desiccation-tolerant strain 4J27 represents a novel species within the genus , for which the name is proposed. The type strain is 4J27 ( = CECT 8257 = LMG 27359).

Funding
This study was supported by the:
  • , Spanish Ministry of Science and Innovation , (Award P07-RNM-02588)
  • , Andalusian Regional Government , (Award CTM2009-09270)
  • , Ministry of Science and Innovation
  • , European Regional Development Funds (EU)
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2013-11-01
2020-09-18
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