1887

Abstract

A novel desiccation-tolerant, xeroprotectant-producing bacterium, designated strain 4J27, was isolated from a rhizosphere subjected to seasonal drought in Granada, Spain. Phylogenetic analysis based on 16S rRNA gene sequencing placed the isolate within the genus , its closest relative being Shep3 DSM 18606, with which it showed 99.23 % 16S rRNA gene sequence similarity. DNA–DNA hybridization measurements showed less than 25 % relatedness between strain 4J27 and DSM 18606. The DNA base composition of strain 4J27 was 65.3 mol%. The main fatty acids were anteiso C, anteiso C, C and iso C and the major menaquinone was MK-9 (H). The peptidoglycan type was A3α with an -Lys–-Ser–-Thr–-Ala interpeptide bridge. The bacterium tested positive for catalase activity and negative for oxidase activity. Phylogenetic, chemotaxonomic and phenotypic analyses indicated that the desiccation-tolerant strain 4J27 represents a novel species within the genus , for which the name is proposed. The type strain is 4J27 ( = CECT 8257 = LMG 27359).

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2013-11-01
2019-10-23
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References

  1. Arakawa T., Timasheff S. N.. ( 1982;). Stabilization of protein structure by sugars. . Biochemistry 21:, 6536–6544. [CrossRef][PubMed]
    [Google Scholar]
  2. Borodina E., Kelly D. P., Schumann P., Rainey F. A., Ward-Rainey N. L., Wood A. P.. ( 2002;). Enzymes of dimethylsulfone metabolism and the phylogenetic characterization of the facultative methylotrophs Arthrobacter sulfonivorans sp. nov., Arthrobacter methylotrophus sp. nov., and Hyphomicrobium sulfonivorans sp. nov.. Arch Microbiol 177:, 173–183. [CrossRef][PubMed]
    [Google Scholar]
  3. Brown A. D.. ( 1976;). Microbial water stress. . Bacteriol Rev 40:, 803–846.[PubMed]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  5. Conn H. J., Dimmick I.. ( 1947;). Soil bacteria similar in morphology to Mycobacterium and Corynebacterium. . J Bacteriol 54:, 291–303.[PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  7. Ding L., Hirose T., Yokota A.. ( 2009;). Four novel Arthrobacter species isolated from filtration substrate. . Int J Syst Evol Microbiol 59:, 856–862. [CrossRef][PubMed]
    [Google Scholar]
  8. DSMZ ( 2001;). Catalogue of Strains, 7th edn. , p. 617. Braunschweig:: DSMZ;. http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Key_to_Murein2.pdf
  9. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Germida J. J., Casida L. E. Jr. ( 1980;). Myceloid growth of Arthrobacter globiformis and other Arthrobacter species. . J Bacteriol 144:, 1152–1158.[PubMed]
    [Google Scholar]
  11. Guindon S., Gascuel O.. ( 2003;). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef][PubMed]
    [Google Scholar]
  12. Huang Y., Zhao N., He L., Wang L., Liu Z., You M., Guan F.. ( 2005;). Arthrobacter scleromae sp. nov. isolated from human clinical specimens. . J Clin Microbiol 43:, 1451–1455. [CrossRef][PubMed]
    [Google Scholar]
  13. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  14. Jones D., Keddie R. M.. ( 1992;). The genus Arthrobacter. . In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, , 2nd edn., vol. 2, pp. 1283–1299. Edited by Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H... NY:: Springer;.
    [Google Scholar]
  15. Julca I., Alaminos M., González-López J., Manzanera M.. ( 2012;). Xeroprotectants for the stabilization of biomaterials. . Biotechnol Adv 30:, 1641–1654. [CrossRef][PubMed]
    [Google Scholar]
  16. Kallimanis A., Kavakiotis K., Perisynakis A., Spröer C., Pukall R., Drainas C., Koukkou A. I.. ( 2009;). Arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium Arthrobacter sp. strain Sphe3. . Int J Syst Evol Microbiol 59:, 275–279. [CrossRef][PubMed]
    [Google Scholar]
  17. Keddie R. M., Collins M. D., Jones D.. ( 1986;). Genus Arthrobacter Conn and Dimmick 1947, 300AL. . In Bergey's Manual of Systematic Bacteriology, pp. 1288–1301. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  18. Kim K. K., Lee K. C., Oh H. M., Kim M. J., Eom M. K., Lee J. S.. ( 2008;). Arthrobacter defluvii sp. nov., 4-chlorophenol-degrading bacteria isolated from sewage. . Int J Syst Evol Microbiol 58:, 1916–1921. [CrossRef][PubMed]
    [Google Scholar]
  19. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  20. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  21. Knapp S., Ladenstein R., Galinski E. A.. ( 1999;). Extrinsic protein stabilization by the naturally occurring osmolytes β-hydroxyectoine and betaine. . Extremophiles 3:, 191–198. [CrossRef][PubMed]
    [Google Scholar]
  22. Kodama Y., Yamamoto H., Amano N., Amachi T.. ( 1992;). Reclassification of two strains of Arthrobacter oxydans and proposal of Arthrobacter nicotinovorans sp. nov.. Int J Syst Bacteriol 42:, 234–239. [CrossRef][PubMed]
    [Google Scholar]
  23. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum. . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  24. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  25. MacKenzie S. L.. ( 1987;). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. . J Assoc Anal Chem 70:, 151–160.[PubMed]
    [Google Scholar]
  26. Manzanera M., García de Castro A., Tøndervik A., Rayner-Brandes M., Strøm A. R., Tunnacliffe A.. ( 2002;). Hydroxyectoine is superior to trehalose for anhydrobiotic engineering of Pseudomonas putida KT2440. . Appl Environ Microbiol 68:, 4328–4333. [CrossRef][PubMed]
    [Google Scholar]
  27. Manzanera M., Vilchez S., Tunnacliffe A.. ( 2004a;). Plastic encapsulation of stabilized Escherichia coli and Pseudomonas putida. . Appl Environ Microbiol 70:, 3143–3145. [CrossRef][PubMed]
    [Google Scholar]
  28. Manzanera M., Vilchez S., Tunnacliffe A.. ( 2004b;). High survival and stability rates of Escherichia coli dried in hydroxyectoine. . FEMS Microbiol Lett 233:, 347–352. [CrossRef][PubMed]
    [Google Scholar]
  29. Mesbah M., Premachandran U., Whitman W.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  30. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  31. Narváez-Reinaldo J. J., Barba I., González-López J., Tunnacliffe A., Manzanera M.. ( 2010;). Rapid method for isolation of desiccation-tolerant strains and xeroprotectants. . Appl Environ Microbiol 76:, 5254–5262. [CrossRef][PubMed]
    [Google Scholar]
  32. National Committee for Clinical Laboratory Standards ( 1997;). Minimum inhibitory concentration (MIC) interpretive standards (g/ml) for organisms other than Haemophilus spp., Neisseria gonorrhoeae, and Streptococcus spp. NCCLS document M7–A4. Wayne, PA:: National Committee for Clinical Laboratory Standards;.
    [Google Scholar]
  33. Piddock L. J. V.. ( 1990;). Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. . J Appl Bacteriol 68:, 307–318. [CrossRef][PubMed]
    [Google Scholar]
  34. Reddy G. S. N., Prakash J. S. S., Matsumoto G. I., Stackebrandt E., Shivaji S.. ( 2002;). Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. . Int J Syst Evol Microbiol 52:, 1017–1021. [CrossRef][PubMed]
    [Google Scholar]
  35. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  36. Schippers-Lammertse A. F., Muijsers A. O., Klatser-Oedekerk K. B.. ( 1963;). Arthrobacter polychromogenes nov. spec., its pigments, and a bacteriophage of this species. . Antonie van Leeuwenhoek 29:, 1–15. [CrossRef]
    [Google Scholar]
  37. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  38. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  39. Sguros P. L.. ( 1955;). Microbial transformations of the tobacco alkaloids. I. Cultural and morphological characteristics of a nicotinophile. . J Bacteriol 69:, 28–37.[PubMed]
    [Google Scholar]
  40. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  41. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  42. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  43. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  44. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  45. Westerberg K., Elväng A. M., Stackebrandt E., Jansson J. K.. ( 2000;). Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol. . Int J Syst Evol Microbiol 50:, 2083–2092. [CrossRef][PubMed]
    [Google Scholar]
  46. Whiton R. S., Lau P., Morgan S. L., Gilbart J., Fox A.. ( 1985;). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. . J Chromatogr A 347:, 109–120. [CrossRef][PubMed]
    [Google Scholar]
  47. Yancey P. H., Clark M. E., Hand S. C., Bowlus R. D., Somero G. N.. ( 1982;). Living with water stress: evolution of osmolyte systems. . Science 217:, 1214–1222. [CrossRef][PubMed]
    [Google Scholar]
  48. Yassin A. F., Spröer C., Siering C., Hupfer H., Schumann P.. ( 2011;). Arthrobacter equi sp. nov., isolated from veterinary clinical material. . Int J Syst Evol Microbiol 61:, 2089–2094. [CrossRef][PubMed]
    [Google Scholar]
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