Oligotrich ciliates are common members of marine microplankton. However, their biodiversity is not well documented. In this study, the morphology and phylogenetic positions of three new oligotrich species, Spirostrombidium apourceolare spec. nov., Spirostrombidium subtropicum spec. nov. and Parallelostrombidium conicum spec. nov., collected from coastal habitats of southern China, were investigated. Spirostrombidium apourceolare is characterized by the girdle kinety which encircles the cell twice as two dextrally oriented whorls with some undulations and by the presence of several macronuclear nodules. Spirostrombidium subtropicum is recognized by the girdle kinety encircling the cell as two dextrally oriented whorls and extrusomes arranged in a stripe along the girdle kinety. Parallelostrombidium conicum differs from its congeners by the obconic body shape and the posterior portion of the girdle kinety extending downwards on the left ventral side to reach the posterior pole. In small-subunit rRNA gene trees, S. subtropicum clusters with Omegastrombidium elegans and Varistrombidium kielum, and Parallelostrombidium conicum is sister to the clade containing Novistrombidium sinicum, Novistrombidium orientale and Parallelostrombidium sp.
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Funding
This study was supported by the:
Natural Science Foundation of China
(Award 31172060, 31201696 and 31222050)
China Postdoctoral Science Foundation
(Award 2012M521376)
Special Program of China Postdoctoral Science Foundation
(Award 2013T60686)
International Research Coordination Network for Biodiversity of Ciliates
(Award IRCN BC-311 1112 0437)
US National Science Foundation
National Natural Science Foundation of China
King Saud University Deanship of Scientific Research, Saudi Arabia
(Award RGP-VPP-083)
AgathaS.(2003). Morphology and ontogenesis of Novistrombidium apsheronicum nov. comb. and Strombidium arenicola (Protozoa, Ciliophora): a comparative light microscopical and SEM study. . Eur J Protistol39, 245–266. [View Article]
AgathaS.(2004a). Evolution of ciliary patterns in the Oligotrichida (Ciliophora, Spirotricha) and its taxonomic implications. . Zoology (Jena)107, 153–168. [View Article][PubMed]
AgathaS.(2011b). Updated hypothesis on the evolution of oligotrichid ciliates (Ciliophora, Spirotricha, Oligotrichida) based on somatic ciliary patterns and ontogenetic data. . Eur J Protistol47, 51–56. [View Article][PubMed]
AlekperovI.,
BuskeyE.,
SnegovayaN.(2007). The free-living ciliates of the Mexican Gulf coast near Port Aransas city and its suburbs (South Texas, USA). . Protistology5, 101–130.
GaoS.,
GongJ.,
LynnD.,
LinX.,
SongW.(2009). An updated phylogeny of oligotrich and choreotrich ciliates (Protozoa, Ciliophora, Spirotrichea) with representative taxa collected from Chinese coastal waters. . Syst Biodivers7, 235–242. [View Article]
GuindonS.,
GascuelO.(2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol52, 696–704. [View Article][PubMed]
HallT. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser41, 95–98.
JiangY.,
YangJ.,
Al-FarrajS. A.,
WarrenA.,
LinX.(2012). Redescriptions of three tintinnid ciliates, Tintinnopsis tocantinensis, T. radix, and T. cylindrica (ciliophora, spirotrichea), from coastal waters off China. . Eur J Protistol48, 314–325. [View Article][PubMed]
LiJ.,
LiuW.,
GaoS.,
WarrenA.,
SongW.(2013). Multigene-based analyses of the phylogenetic evolution of oligotrich ciliates, with consideration of the internal transcribed spacer 2 secondary structure of three systematically ambiguous genera. . Eukaryot Cell12, 430–437. [View Article][PubMed]
LiuW.,
XuD.,
LinX.,
LiJ.,
GongJ.,
Al-RasheidK. A. S.,
SongW.(2009).Novistrombidium sinicum n. sp. and Novistrombidium orientale n. sp. (Protozoa: Ciliophora): Two new oligotrich ciliates from a Mangrove Wetland, South China. . J Eukaryot Microbiol56, 459–465. [View Article][PubMed]
LiuW.,
YiZ.,
LinX.,
Al-RasheidK. A. S.(2011a). Morphologic and molecular data suggest that Lynnella semiglobulosa n. g., n. sp. represents a new family within the subclass Choreotrichia (Ciliophora, Spirotrichea). . J Eukaryot Microbiol58, 43–49. [View Article][PubMed]
LiuW.,
YiZ.,
WarrenA.,
Al-RasheidK. A. S.,
Al-FarrajS. A.,
LinX.,
SongW.(2011b). Taxonomy, morphology and molecular systematics of a new oligotrich ciliates, Williophrya maedai gen. nov., sp. nov., with redescriptions of Strombidium basimorphum and Pseudotontonia simplicidens (Protozoa, Ciliophora, Oligotrichia). . Syst Biodivers9, 247–258. [View Article]
LiuW.,
YiZ.,
LinX.,
WarrenA.,
SongW.(2012). Phylogeny of three choreotrich genera (Protozoa, Ciliophora, Spirotrichea), with morphological, morphogenetic and molecular investigations on three strobilidiid species. . Zool Scr41, 417–434. [View Article]
MontagnesD.,
TaylorF.(1994). The salient features of five marine ciliates in the class Spirotrichea (Oligotrichia), with notes on their culturing and behaviour. . J Eukaryot Microbiol41, 569–586. [View Article]
NylanderJ. A.(2004). MrModeltest Ver.2.. Distributed by the author. Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University;, Sweden:.
PetzW.,
SongW.,
WilbertN.(1995). Taxonomy and ecology of the ciliate fauna (Protozoa, Ciliophora) in the endopagial and pelagial of the Weddell Sea, Antarctica. . Stapfia40, 1–223.
SongW.,
BradburyP.(1998). Studies on some new and rare reported marine planktonic ciliates (Ciliophora: Oligotrichia) from coastal waters in North China. . J Mar Biol Assoc U K78, 767–794. [View Article]
SongW.,
ZhuM.,
ChenZ.(1999a). Updating the systematics of the planktonic oligotrichous ciliates (Ciliophora, Protozoa) with description of ciliature patterns at generic level. . The Yellow Sea5, 77–85.
SongW.,
WilbertN.,
WarrenA.(1999b). Three new entocommensal ciliates from digestive tract of sea urchins of the Weddell Sea, Antarctica (Protozoa, Ciliophora). . Polar Biol22, 232–240. [View Article]
SongW.,
LiJ.,
LiuW.,
JiangJ.,
Al-RasheidK. A. S.,
HuX.(2013). Taxonomy, morphology and molecular systematics of three oligotrich ciliates, including a description of Apostrombidium parakielum spec. nov. (Ciliophora, Oligotrichia). . Int J Syst Evol Microbiol63, 1179–1191. [View Article][PubMed]
XuD.,
SongW.,
LinX.,
WarrenA.(2006a). On two marine oligotrich ciliates, Spirostrombidium agathae spec. nov. and S. schizostomum (Kahl, 1932) n. comb. from China, with a key to the identification of seven well-characterized Spirostrombidium spp. (Ciliophora: Oligotrichida). . Acta Protozool45, 433–442.
XuD.,
SongW.,
WarrenA.(2006b). Morphology and infraciliature of two new species of marine oligotrich ciliates (Ciliophora: Oligotrichida) from China. . J Nat Hist40, 1287–1299. [View Article]
XuD.,
WarrenA.,
SongW.(2009). Oligotrichs. . In Free-living Ciliates in the Bohai and Yellow Seas, pp. 307–351. Edited by
SongW.,
WarrenA.,
HuX.
. Beijing:: Science Press;.
ZhangQ.,
YiZ.,
XuD.,
Al-RasheidK. A. S.,
GongJ.,
SongW.(2010). Molecular phylogeny of oligotrich genera Omegastrombidium and Novistrombidium (Protozoa, Ciliophora) for the systematical relationships within Family Strombidiidae. . Chin J Oceanology Limnol28, 769–777. [View Article]