Taxonomy, morphology and phylogeny of three new oligotrich ciliates (Protozoa, Ciliophora, Oligotrichia) from southern China Free

Abstract

Oligotrich ciliates are common members of marine microplankton. However, their biodiversity is not well documented. In this study, the morphology and phylogenetic positions of three new oligotrich species, spec. nov., spec. nov. and spec. nov., collected from coastal habitats of southern China, were investigated. is characterized by the girdle kinety which encircles the cell twice as two dextrally oriented whorls with some undulations and by the presence of several macronuclear nodules. is recognized by the girdle kinety encircling the cell as two dextrally oriented whorls and extrusomes arranged in a stripe along the girdle kinety. differs from its congeners by the obconic body shape and the posterior portion of the girdle kinety extending downwards on the left ventral side to reach the posterior pole. In small-subunit rRNA gene trees, clusters with and , and is sister to the clade containing , and sp.

Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 31172060, 31201696 and 31222050)
  • China Postdoctoral Science Foundation (Award 2012M521376)
  • Special Program of China Postdoctoral Science Foundation (Award 2013T60686)
  • International Research Coordination Network for Biodiversity of Ciliates (Award IRCN BC-311 1112 0437)
  • US National Science Foundation
  • National Natural Science Foundation of China
  • King Saud University Deanship of Scientific Research, Saudi Arabia (Award RGP-VPP-083)
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2013-12-01
2024-03-29
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References

  1. Agatha S. ( 2003 ). Morphology and ontogenesis of Novistrombidium apsheronicum nov. comb. and Strombidium arenicola (Protozoa, Ciliophora): a comparative light microscopical and SEM study. . Eur J Protistol 39, 245266. [View Article]
    [Google Scholar]
  2. Agatha S. ( 2004a ). Evolution of ciliary patterns in the Oligotrichida (Ciliophora, Spirotricha) and its taxonomic implications. . Zoology (Jena) 107, 153168. [View Article] [PubMed]
    [Google Scholar]
  3. Agatha S. ( 2004b ). A cladistic approach for the classification of oligotrichid ciliates (Ciliophora: Spirotricha). . Acta Protozool 43, 201217.[PubMed]
    [Google Scholar]
  4. Agatha S. ( 2011a ). Global diversity of aloricate Oligotrichea (Protista, Ciliophora, Spirotricha) in marine and brackish sea water. . PLoS ONE 6, e22466. [View Article] [PubMed]
    [Google Scholar]
  5. Agatha S. ( 2011b ). Updated hypothesis on the evolution of oligotrichid ciliates (Ciliophora, Spirotricha, Oligotrichida) based on somatic ciliary patterns and ontogenetic data. . Eur J Protistol 47, 5156. [View Article] [PubMed]
    [Google Scholar]
  6. Agatha S., Strüder-Kypke M. C. ( 2012 ). Reconciling cladistic and genetic analyses in choreotrichid ciliates (Ciliophora, Spirotricha, Oligotrichea). . J Eukaryot Microbiol 59, 325350. [View Article] [PubMed]
    [Google Scholar]
  7. Alekperov I., Buskey E., Snegovaya N. ( 2007 ). The free-living ciliates of the Mexican Gulf coast near Port Aransas city and its suburbs (South Texas, USA). . Protistology 5, 101130.
    [Google Scholar]
  8. Dale T., Dahl E. ( 1987 ). Mass occurrence of planktonic oligotrichous ciliates in a bay in southern Norway. . J Plankton Res 9, 871879. [View Article]
    [Google Scholar]
  9. Fenchel T. ( 2008 ). The microbial loop – 25 years later. . J Exp Mar Biol Ecol 366, 99103. [View Article]
    [Google Scholar]
  10. Gao S., Gong J., Lynn D., Lin X., Song W. ( 2009 ). An updated phylogeny of oligotrich and choreotrich ciliates (Protozoa, Ciliophora, Spirotrichea) with representative taxa collected from Chinese coastal waters. . Syst Biodivers 7, 235242. [View Article]
    [Google Scholar]
  11. Guindon S., Gascuel O. ( 2003 ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52, 696704. [View Article] [PubMed]
    [Google Scholar]
  12. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  13. Jeanmougin F., Thompson J. D., Gouy M., Higgins D. G., Gibson T. J. ( 1998 ). Multiple sequence alignment with clustal_x . . Trends Biochem Sci 23, 403405. [View Article] [PubMed]
    [Google Scholar]
  14. Jiang Y., Yang J., Al-Farraj S. A., Warren A., Lin X. ( 2012 ). Redescriptions of three tintinnid ciliates, Tintinnopsis tocantinensis, T. radix, and T. cylindrica (ciliophora, spirotrichea), from coastal waters off China. . Eur J Protistol 48, 314325. [View Article] [PubMed]
    [Google Scholar]
  15. Kim Y. O., Kim S. Y., Lee W. J., Choi J. K. ( 2010 ). New observations on the choreotrich ciliate Strombidinopsis acuminata Fauré-Fremiet 1924, and comparison with Strombidinopsis jeokjo Jeong et al., 2004. . J Eukaryot Microbiol 57, 4855. [View Article] [PubMed]
    [Google Scholar]
  16. Lei Y., Xu K., Song W. ( 1999 ). Free-living ciliates from marine farming ponds. . In: Progress in Protozoology, pp. 269295. Edited by Song W., Xu K., Shi X., Hu X., Lei Y., Wei J., Chen Z., Shi X., Wang M. . Qingdao:: Qingdao Ocean University Press; (in Chinese).
    [Google Scholar]
  17. Li J., Liu W., Gao S., Warren A., Song W. ( 2013 ). Multigene-based analyses of the phylogenetic evolution of oligotrich ciliates, with consideration of the internal transcribed spacer 2 secondary structure of three systematically ambiguous genera. . Eukaryot Cell 12, 430437. [View Article] [PubMed]
    [Google Scholar]
  18. Liu W., Xu D., Lin X., Li J., Gong J., Al-Rasheid K. A. S., Song W. ( 2009 ). Novistrombidium sinicum n. sp. and Novistrombidium orientale n. sp. (Protozoa: Ciliophora): Two new oligotrich ciliates from a Mangrove Wetland, South China. . J Eukaryot Microbiol 56, 459465. [View Article] [PubMed]
    [Google Scholar]
  19. Liu W., Yi Z., Lin X., Al-Rasheid K. A. S. ( 2011a ). Morphologic and molecular data suggest that Lynnella semiglobulosa n. g., n. sp. represents a new family within the subclass Choreotrichia (Ciliophora, Spirotrichea). . J Eukaryot Microbiol 58, 4349. [View Article] [PubMed]
    [Google Scholar]
  20. Liu W., Yi Z., Warren A., Al-Rasheid K. A. S., Al-Farraj S. A., Lin X., Song W. ( 2011b ). Taxonomy, morphology and molecular systematics of a new oligotrich ciliates, Williophrya maedai gen. nov., sp. nov., with redescriptions of Strombidium basimorphum and Pseudotontonia simplicidens (Protozoa, Ciliophora, Oligotrichia). . Syst Biodivers 9, 247258. [View Article]
    [Google Scholar]
  21. Liu W., Yi Z., Lin X., Warren A., Song W. ( 2012 ). Phylogeny of three choreotrich genera (Protozoa, Ciliophora, Spirotrichea), with morphological, morphogenetic and molecular investigations on three strobilidiid species. . Zool Scr 41, 417434. [View Article]
    [Google Scholar]
  22. Lynn D. H. ( 2008 ). The Ciliated Protozoa: Characterization, Classification, and Guide to the Literature, , 3rd edn.. Dordrecht:: Springer;.
    [Google Scholar]
  23. Medlin L., Elwood H. J., Stickel S., Sogin M. L. ( 1988 ). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. . Gene 71, 491499. [View Article] [PubMed]
    [Google Scholar]
  24. Montagnes D., Taylor F. ( 1994 ). The salient features of five marine ciliates in the class Spirotrichea (Oligotrichia), with notes on their culturing and behaviour. . J Eukaryot Microbiol 41, 569586. [View Article]
    [Google Scholar]
  25. Nylander J. A. ( 2004 ). MrModeltest Ver.2.. Distributed by the author. Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University;, Sweden:.
    [Google Scholar]
  26. Page R. D. M. ( 1996 ). TreeView: an application to display phylogenetic trees on personal computers. . Comput Appl Biosci 12, 357358.[PubMed]
    [Google Scholar]
  27. Petz W., Song W., Wilbert N. ( 1995 ). Taxonomy and ecology of the ciliate fauna (Protozoa, Ciliophora) in the endopagial and pelagial of the Weddell Sea, Antarctica. . Stapfia 40, 1223.
    [Google Scholar]
  28. Pierce R. W., Turner J. T. ( 1992 ). Ecology of planktonic ciliates in marine food webs. . Rev Aquat Sci 6, 139181.
    [Google Scholar]
  29. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [View Article] [PubMed]
    [Google Scholar]
  30. Song W., Bradbury P. ( 1998 ). Studies on some new and rare reported marine planktonic ciliates (Ciliophora: Oligotrichia) from coastal waters in North China. . J Mar Biol Assoc U K 78, 767794. [View Article]
    [Google Scholar]
  31. Song W., Zhu M., Chen Z. ( 1999a ). Updating the systematics of the planktonic oligotrichous ciliates (Ciliophora, Protozoa) with description of ciliature patterns at generic level. . The Yellow Sea 5, 7785.
    [Google Scholar]
  32. Song W., Wilbert N., Warren A. ( 1999b ). Three new entocommensal ciliates from digestive tract of sea urchins of the Weddell Sea, Antarctica (Protozoa, Ciliophora). . Polar Biol 22, 232240. [View Article]
    [Google Scholar]
  33. Song W., Li J., Liu W., Jiang J., Al-Rasheid K. A. S., Hu X. ( 2013 ). Taxonomy, morphology and molecular systematics of three oligotrich ciliates, including a description of Apostrombidium parakielum spec. nov. (Ciliophora, Oligotrichia). . Int J Syst Evol Microbiol 63, 11791191. [View Article] [PubMed]
    [Google Scholar]
  34. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  35. Wilbert N. ( 1975 ). Eine verbesserte Technik der Protargolimprägnation für Ciliaten. . Mikrokosmos 64, 171179 (in German).
    [Google Scholar]
  36. Xu D., Song W. ( 2006 ). Hapantotypification and morphological redescription of the marine planktonic ciliate, Spirostrombidium cinctum (Kahl, 1932) Petz, Song et Wilbert, 1995 (Ciliophora: Oligotrichida). . Acta Protozool 45, 1725.
    [Google Scholar]
  37. Xu D., Song W., Lin X., Warren A. ( 2006a ). On two marine oligotrich ciliates, Spirostrombidium agathae spec. nov. and S. schizostomum (Kahl, 1932) n. comb. from China, with a key to the identification of seven well-characterized Spirostrombidium spp. (Ciliophora: Oligotrichida). . Acta Protozool 45, 433442.
    [Google Scholar]
  38. Xu D., Song W., Warren A. ( 2006b ). Morphology and infraciliature of two new species of marine oligotrich ciliates (Ciliophora: Oligotrichida) from China. . J Nat Hist 40, 12871299. [View Article]
    [Google Scholar]
  39. Xu D., Warren A., Song W. ( 2009 ). Oligotrichs. . In Free-living Ciliates in the Bohai and Yellow Seas, pp. 307351. Edited by Song W., Warren A., Hu X. . Beijing:: Science Press;.
    [Google Scholar]
  40. Zhang Q., Yi Z., Xu D., Al-Rasheid K. A. S., Gong J., Song W. ( 2010 ). Molecular phylogeny of oligotrich genera Omegastrombidium and Novistrombidium (Protozoa, Ciliophora) for the systematical relationships within Family Strombidiidae. . Chin J Oceanology Limnol 28, 769777. [View Article]
    [Google Scholar]
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