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Abstract

Four Gram-staining-positive, catalase-negative, coccoid isolates, designated NTUH_1465, NTUH_2196, NTUH_4957 and NTUH_5572, were isolated from human specimens. The four isolates displayed more than 99.6 % 16S rRNA gene sequence similarity with ATCC 10379, and 96.7 to 98.6 % similarity with ATCC 700632, ATCC 27824 or CCUG 42726. However, phylogenetic analysis of concatenated sequences of three housekeeping genes, , and , suggested that the four isolates were distinct from ATCC 10379 and other species. Isolates NTUH_2196, NTUH_4957 and NTUH_5572 clustered together and formed a stable monophyletic clade. DNA–DNA hybridization values among strains NTUH_1465 and NTUH_5572 and their phylogenetically related neighbours were all lower than 49 %. The four isolates could be distinguished from and other species by phenotypic characteristics. Based on the phylogenetic and phenotypic results, two novel species sp. nov. (type strain NTUH_1465 = BCRC 80365 = JCM 18067) and sp. nov. (type strain NTUH_5572 = BCRC 80366 = JCM 18066) are proposed.

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2014-06-01
2019-10-15
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References

  1. Anil M., Ozkalay N., Helvaci M., Agus N., Guler O., Dikerler A., Kanar B.. ( 2007;). Meningitis due to Gemella haemolysans in a pediatric case. . J Clin Microbiol 45:, 2337–2339. [CrossRef][PubMed]
    [Google Scholar]
  2. Berger U.. ( 1961;). A proposed new genus of Gram-negative cocci: Gemella. . Int Bull Bacteriol Nomencl Taxon 11:, 17–19. [CrossRef]
    [Google Scholar]
  3. Brooks J. B., Kellogg D. S., Thacker L., Turner E. M.. ( 1971;). Analysis by gas chromatography of fatty acids found in whole cultural extracts of Neisseria species. . Can J Microbiol 17:, 531–543. [CrossRef][PubMed]
    [Google Scholar]
  4. Brouqui P., Raoult D.. ( 2001;). Endocarditis due to rare and fastidious bacteria. . Clin Microbiol Rev 14:, 177–207. [CrossRef][PubMed]
    [Google Scholar]
  5. Cerdá Zolezzi P., Laplana L. M., Calvo C. R., Cepero P. G., Erazo M. C., Gómez-Lus R.. ( 2004;). Molecular basis of resistance to macrolides and other antibiotics in commensal viridans group streptococci and Gemella spp. and transfer of resistance genes to Streptococcus pneumoniae. . Antimicrob Agents Chemother 48:, 3462–3467. [CrossRef][PubMed]
    [Google Scholar]
  6. CLSI ( 2010;). Performance Standards for Antimicrobial Susceptibility Testing; 20th Informational Supplement M100-S20. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  7. Collins M. D.. ( 2006;). The Genus Gemella. . In The Prokaryotes, , 3rd edn., pp. 511–518. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Collins M. D., Hutson R. A., Falsen E., Sjöden B., Facklam R. R.. ( 1998a;). Description of Gemella sanguinis sp. nov., isolated from human clinical specimens. . J Clin Microbiol 36:, 3090–3093.[PubMed]
    [Google Scholar]
  9. Collins M. D., Hutson R. A., Falsen E., Sjöden B., Facklam R. R.. ( 1998b;). Gemella bergeriae sp. nov., isolated from human clinical specimens. . J Clin Microbiol 36:, 1290–1293.[PubMed]
    [Google Scholar]
  10. Collins M. D., Rodriguez Jovita M., Foster G., Sjödén B., Falsen E.. ( 1999;). Characterization of a Gemella-like organism from the oral cavity of a dog: description of Gemella palaticanis sp. nov.. Int J Syst Bacteriol 49:, 1523–1526. [CrossRef][PubMed]
    [Google Scholar]
  11. Durack D. T., Kaplan E. L., Bisno A. L.. ( 1983;). Apparent failures of endocarditis prophylaxis. Analysis of 52 cases submitted to a national registry. . JAMA 250:, 2318–2322. [CrossRef][PubMed]
    [Google Scholar]
  12. Gevers D., Cohan F. M., Lawrence J. G., Spratt B. G., Coenye T., Feil E. J., Stackebrandt E., Van de Peer Y., Vandamme P.. & other authors ( 2005;). Opinion: re-evaluating prokaryotic species. . Nat Rev Microbiol 3:, 733–739. [CrossRef][PubMed]
    [Google Scholar]
  13. Hanage W. P., Fraser C., Spratt B. G.. ( 2006;). Sequences, sequence clusters and bacterial species. . Philos Trans R Soc Lond B Biol Sci 361:, 1917–1927. [CrossRef][PubMed]
    [Google Scholar]
  14. Hoyles L., Foster G., Falsen E., Collins M. D.. ( 2000;). Characterization of a Gemella-like organism isolated from an abscess of a rabbit: description of Gemella cunicula sp. nov.. Int J Syst Evol Microbiol 50:, 2037–2041. [CrossRef][PubMed]
    [Google Scholar]
  15. Hung W.-C., Tseng S.-P., Chen H.-J., Tsai J.-C., Chang C.-H., Lee T.-F., Hsueh P.-R., Teng L.-J.. ( 2010;). Use of groESL as a target for identification of Abiotrophia, Granulicatella, and Gemella species. . J Clin Microbiol 48:, 3532–3538. [CrossRef][PubMed]
    [Google Scholar]
  16. Jean W. D., Leu T.-Y., Lee C.-Y., Chu T.-J., Lin S. Y., Shieh W. Y.. ( 2009;). Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. . Int J Syst Evol Microbiol 59:, 53–59. [CrossRef][PubMed]
    [Google Scholar]
  17. Kilpper-Bälz R., Schleifer K. H.. ( 1988;). Transfer of Streptococcus morbillorum to the genus Gemella as Gemella morbillorum comb. nov.. Int J Syst Bacteriol 38:, 442–443. [CrossRef]
    [Google Scholar]
  18. Konstantinidis K. T., Ramette A., Tiedje J. M.. ( 2006;). Toward a more robust assessment of intraspecies diversity, using fewer genetic markers. . Appl Environ Microbiol 72:, 7286–7293. [CrossRef][PubMed]
    [Google Scholar]
  19. La Scola B., Raoult D.. ( 1998;). Molecular identification of Gemella species from three patients with endocarditis. . J Clin Microbiol 36:, 866–871.[PubMed]
    [Google Scholar]
  20. Malik I., Ghosh S., Nutt C., Macdonald A., Bal A. M., Collier A.. ( 2010;). Gemella haemolysans bacteraemia in a patient with solitary liver abscess. . J Microbiol Immunol Infect 43:, 438–441. [CrossRef][PubMed]
    [Google Scholar]
  21. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A., Kämpfer P., Maiden M. C., Nesme X., Rosselló-Mora R., Swings J.. & other authors ( 2002;). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef][PubMed]
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  23. Thjötta T., Böe J.. ( 1938;). Neisseria haemolysans. A haemolytic species of Neisseria Trevisan. . Acta Pathol Microbiol Scand Suppl 37:, 727–731.
    [Google Scholar]
  24. Thompson C. C., Thompson F. L., Vandemeulebroecke K., Hoste B., Dawyndt P., Swings J.. ( 2004;). Use of recA as an alternative phylogenetic marker in the family Vibrionaceae. . Int J Syst Evol Microbiol 54:, 919–924. [CrossRef][PubMed]
    [Google Scholar]
  25. Thompson F. L., Gevers D., Thompson C. C., Dawyndt P., Naser S., Hoste B., Munn C. B., Swings J.. ( 2005;). Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. . Appl Environ Microbiol 71:, 5107–5115. [CrossRef][PubMed]
    [Google Scholar]
  26. Ulger-Toprak N., Summanen P. H., Liu C., Rowlinson M. C., Finegold S. M.. ( 2010;). Gemella asaccharolytica sp. nov., isolated from human clinical specimens. . Int J Syst Evol Microbiol 60:, 1023–1026. [CrossRef][PubMed]
    [Google Scholar]
  27. Vossen M. G., Gattringer K. B., Khalifeh N., Koreny M., Spertini V., Mallouhi A., Willeit M., Volc-Platzer B., Asboth F.. & other authors ( 2012;). Gemella morbillorum bacteremia after anti-tumor necrosis factor alpha as acne inversa therapy. . J Clin Microbiol 50:, 1109–1112. [CrossRef][PubMed]
    [Google Scholar]
  28. Woo P. C., To A. P., Lau S. K., Fung A. M., Yuen K. Y.. ( 2004;). Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia. . J Clin Microbiol 42:, 3303–3305. [CrossRef][PubMed]
    [Google Scholar]
  29. Yamakawa T., Ueta N.. ( 1964;). Gaschromatographic studies of microbial components. I. Carbohydrate and fatty ccid constitution of Neisseria. . Jpn J Exp Med 34:, 361–374.[PubMed]
    [Google Scholar]
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