1887

Abstract

Four Gram-staining-positive, catalase-negative, coccoid isolates, designated NTUH_1465, NTUH_2196, NTUH_4957 and NTUH_5572, were isolated from human specimens. The four isolates displayed more than 99.6 % 16S rRNA gene sequence similarity with ATCC 10379, and 96.7 to 98.6 % similarity with ATCC 700632, ATCC 27824 or CCUG 42726. However, phylogenetic analysis of concatenated sequences of three housekeeping genes, , and , suggested that the four isolates were distinct from ATCC 10379 and other species. Isolates NTUH_2196, NTUH_4957 and NTUH_5572 clustered together and formed a stable monophyletic clade. DNA–DNA hybridization values among strains NTUH_1465 and NTUH_5572 and their phylogenetically related neighbours were all lower than 49 %. The four isolates could be distinguished from and other species by phenotypic characteristics. Based on the phylogenetic and phenotypic results, two novel species sp. nov. (type strain NTUH_1465 = BCRC 80365 = JCM 18067) and sp. nov. (type strain NTUH_5572 = BCRC 80366 = JCM 18066) are proposed.

Funding
This study was supported by the:
  • , National Science Council of Taiwan , (Award NSC 97-2320-B-002-023-MY3)
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2014-06-01
2020-07-08
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