1887

Abstract

A Gram-stain-negative, fusiform-shaped, facultatively anaerobic bacterial strain, designated EBTL04, was isolated from activated sludge using algal metabolites and taxonomically characterized through polyphasic investigation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EBTL04 belongs to the family , class , and is closely related to DSM 12251 (98.6 % sequence similarity), ATCC 25309 (97.9 %), DSM 14044 (97.5 %), DSM 12252 (94.7 %) and KCTC 22182 (93.7 %). The G+C content of the genomic DNA of strain EBTL04 was 62.7 mol%. The menaquinone MK-6 was detected as the predominant quinone. Strain EBTL04 contained phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine as major polar lipids. A fatty acid profile with Cω5, iso-C, C, anteiso-C and C as the major components supported the classification of strain EBTL04 in the genus . Based on several phenotypic, genotypic and chemotaxonomic features, strain EBTL04 was clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL04 should be considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EBTL04 ( = KCTC 23681 = JCM 18053).

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2014-02-01
2019-12-14
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