1887

Abstract

A nitrogen-fixing bacterium, designated strain SCSIO N0430, was isolated from a mangrove sediment sample. Analysis of the sequence of the gene responsible for nitrogen fixation in this strain indicated a close relationship to an uncultured bacterium ZNZ-D11 (GenBank accession no. JF896696). 16S rRNA gene sequence analysis revealed that this isolate had less than 93 % similarity to its closest relative, DQHS4. A phylogenetic tree reconstructed based on 16S rRNA gene sequences revealed that strain SCSIO N0430 was a member of the phylum . Chemotaxonomic and physiological characteristics, including phospholipids and major fatty acids, readily distinguished the isolate from established members of the phylum It is concluded that strain SCSIO N0430 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed, with the type strain of the species SCSIO N0430 ( = KCTC 32129 = DSM 27148 = JCM 19152). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the three genera , and are proposed to make up a novel family, fam. nov., in the order .

Funding
This study was supported by the:
  • , MOST , (Award 2010CB833801 and 2012AA092104)
  • , NSFC , (Award 41276113, 41276004 and 40906075)
  • , National Science and Technology Supporting Program , (Award 2009BAB44B03)
  • , Knowledge Innovation Program of Chinese Academy of Sciences , (Award KSCX2-EW-G-12)
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2014-03-01
2020-08-13
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