1887

Abstract

During the characterization of the mycobiota associated with shallow-water marine environments from Antarctic sea, a novel pink yeast species was isolated. Sequence analysis of the D1/D2 domain of the LSU rDNA gene and 5.8S-ITS regions revealed that the isolated yeast was closely related to CBS 320 and CBS 9124. On the basis of morphological, biochemical and physiological characterization and phylogenetic analyses, a novel basidiomycetous yeast species, sp. nov., is proposed. The type strain is Pi2 ( = CBS 12733  = CECT 13081) which was isolated from shallow-water marine sediment in Fildes Bay, King George Island, Antarctica.

Funding
This study was supported by the:
  • Recursos y Tecnologías Agrarias in coordination with the Comunidades Autónomas del Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica 2004–2007
  • European Social Fund (Award DICYT-USACH, INACH G_06-10, PBCT PDA 13 and Fondecyt 11090192)
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2013-10-01
2024-04-18
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [View Article] [PubMed]
    [Google Scholar]
  2. Bai F. Y., Takashima M., Nakase T. ( 2001 ). Phylogenetic analysis of strains originally assigned to Bullera variabilis: descriptions of Bullera pseudohuiaensis sp. nov., Bullera komagatae sp. nov. and Bullera pseudoschimicola sp. nov. . Int J Syst Evol Microbiol 51, 21772187. [View Article] [PubMed]
    [Google Scholar]
  3. Bauer R., Begerow D., Sampaio J. P., Weiß M., Oberwinkler F. ( 2006 ). The simple-septate basidiomycetes: a synopsis. . Mycol Prog 5, 4166. [View Article]
    [Google Scholar]
  4. Bhadra B., Rao R. S., Singh P. K., Sarkar P. K., Shivaji S. ( 2008 ). Yeasts and yeast-like fungi associated with tree bark: diversity and identification of yeasts producing extracellular endoxylanases. . Curr Microbiol 56, 489494. [View Article] [PubMed]
    [Google Scholar]
  5. Butinar L., Santos S., Spencer-Martins I., Oren A., Gunde-Cimerman N. ( 2005 ). Yeast diversity in hypersaline habitats. . FEMS Microbiol Lett 244, 229234. [View Article] [PubMed]
    [Google Scholar]
  6. Buzzini P., Branda E., Goretti M., Turchetti B. ( 2012 ). Psychrophilic yeasts from worldwide glacial habitats: diversity, adaptation strategies and biotechnological potential. . FEMS Microbiol Ecol 82, 217241. [View Article] [PubMed]
    [Google Scholar]
  7. Carrasco M., Rozas J. M., Barahona S., Alcaíno J., Cifuentes V., Baeza M. ( 2012 ). Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. . BMC Microbiol 12, 251. [View Article] [PubMed]
    [Google Scholar]
  8. de García V., Brizzio S., Libkind D., Buzzini P., van Broock M. ( 2007 ). Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina. . FEMS Microbiol Ecol 59, 331341. [View Article] [PubMed]
    [Google Scholar]
  9. de Garcia V., Brizzio S., van Broock M. R. ( 2012 ). Yeasts from glacial ice of Patagonian Andes, Argentina. . FEMS Microbiol Ecol 82, 540550. [View Article] [PubMed]
    [Google Scholar]
  10. Fell J. W. ( 2012 ). Yeasts in marine environments. . In Marine Fungi and Fungal-like Organisms, pp. 91102. Edited by Jones E. B. G., Pang K.-L. . Berlin:: De Gruyter;. [View Article]
    [Google Scholar]
  11. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. ( 2000 ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. . Int J Syst Evol Microbiol 50, 13511371. [View Article] [PubMed]
    [Google Scholar]
  12. Gadanho M., Almeida J. M., Sampaio J. P. ( 2003 ). Assessment of yeast diversity in a marine environment in the south of Portugal by microsatellite-primed PCR. . Antonie van Leeuwenhoek 84, 217227. [View Article] [PubMed]
    [Google Scholar]
  13. Hamamoto M., Nagahama T., Tamura M. ( 2002 ). Systematic study of basidiomycetous yeasts - evaluation of the ITS regions of rDNA to delimit species of the genus Rhodosporidium . . FEMS Yeast Res 2, 409413.[PubMed]
    [Google Scholar]
  14. Hua M. X., Chi Z., Liu G. L., Buzdar M. A., Chi Z. M. ( 2010 ). Production of a novel and cold-active killer toxin by Mrakia frigida 2E00797 isolated from sea sediment in Antarctica. . Extremophiles 14, 515521. [View Article] [PubMed]
    [Google Scholar]
  15. Janisiewicz W. J., Kurtzman C. P., Buyer J. S. ( 2010 ). Yeasts associated with nectarines and their potential for biological control of brown rot. . Yeast 27, 389398. [View Article] [PubMed]
    [Google Scholar]
  16. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  17. Kurtzman C. P., Robnett C. J. ( 1998 ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73, 331371. [View Article] [PubMed]
    [Google Scholar]
  18. Kutty S. N., Philip R. ( 2008 ). Marine yeasts-a review. . Yeast 25, 465483. [View Article] [PubMed]
    [Google Scholar]
  19. Laitila A., Wilhelmson A., Kotaviita E., Olkku J., Home S., Juvonen R. J. ( 2006 ). Yeasts in an industrial malting ecosystem. . J Ind Microbiol Biotechnol 33, 953966. [View Article] [PubMed]
    [Google Scholar]
  20. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). Clustal W and Clustal X version 2.0. . Bioinformatics 23, 29472948. [View Article] [PubMed]
    [Google Scholar]
  21. Libkind D., Brizzio S., Ruffini A., Gadanho M., van Broock M., Paulo Sampaio J. ( 2003 ). Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia, Argentina. . Antonie van Leeuwenhoek 84, 313322. [View Article] [PubMed]
    [Google Scholar]
  22. Libkind D., Sampaio J. P., van Broock M. ( 2010 ). Cystobasidiomycetes yeasts from Patagonia (Argentina): description of Rhodotorula meli sp. nov. from glacial meltwater. . Int J Syst Evol Microbiol 60, 22512256. [View Article] [PubMed]
    [Google Scholar]
  23. Loque C. P., Medeiros A. O., Pellizzari F. M., Oliveira E. C., Rosa C. A., Rosa L. H. ( 2010 ). Fungal community associated with marine macroalgae from Antarctica. . Polar Biol 33, 641648. [View Article]
    [Google Scholar]
  24. Margesin R., Fonteyne P. A., Schinner F., Sampaio J. P. ( 2007 ). Rhodotorula psychrophila sp. nov., Rhodotorula psychrophenolica sp. nov. and Rhodotorula glacialis sp. nov., novel psychrophilic basidiomycetous yeast species isolated from alpine environments. . Int J Syst Evol Microbiol 57, 21792184. [View Article] [PubMed]
    [Google Scholar]
  25. Nagahama T. ( 2006 ). Yeast biodiversity in freshwater, marine and deep-sea environments. . In Biodiversity and Ecophysiology of Yeasts, pp. 241262. Edited by Rosa C. A., Gábor P. . Berlin:: Springer-Verlag;. [View Article]
    [Google Scholar]
  26. Nagahama T., Hamamoto M., Nakase T., Takami H., Horikoshi K. ( 2001 ). Distribution and identification of red yeasts in deep-sea environments around the northwest Pacific Ocean. . Antonie van Leeuwenhoek 80, 101110. [View Article] [PubMed]
    [Google Scholar]
  27. Nagahama T., Hamamoto M., Nakase T., Horikoshi K. ( 2003 ). Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically. . Int J Syst Evol Microbiol 53, 897903. [View Article] [PubMed]
    [Google Scholar]
  28. Nagano Y., Nagahama T. ( 2012 ). Fungal diversity in deep-sea extreme environments. . Fungal Ecol 5, 463471. [View Article]
    [Google Scholar]
  29. Nisiotou A. A., Gibson G. R. ( 2005 ). Isolation of culturable yeasts from market wines and evaluation of the 5.8S-ITS rDNA sequence analysis for identification purposes. . Lett Appl Microbiol 41, 454463. [View Article] [PubMed]
    [Google Scholar]
  30. Prakitchaiwattana C. J., Fleet G. H., Heard G. M. ( 2004 ). Application and evaluation of denaturing gradient gel electrophoresis to analyse the yeast ecology of wine grapes. . FEMS Yeast Res 4, 865877. [View Article] [PubMed]
    [Google Scholar]
  31. Sakurai H., Sato T., Arai H., Takasaki A., Tada S., Hori H., Kimpara I., Matsuyama T., Kodama M. ( 1996 ). Habitats of fish and epibenthic invertebrates in Fildes Bay, King George Island, Antarctica. . Proc NIPR Symp Polar Biol 9, 231242.
    [Google Scholar]
  32. Sampaio J. P. ( 2011 ). Rhodotorula Harrison (1928). . In The Yeasts, a Taxonomic Study, , 5th edn., vol. 3, pp. 18731927. Edited by Kurtzman C. P., Fell J. W., Boekhout T. . New York:: Elsevier;. [View Article]
    [Google Scholar]
  33. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. ( 2002 ). Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. . FEMS Yeast Res 2, 495517.[PubMed] [CrossRef]
    [Google Scholar]
  34. Shivaji S., Prasad G. S. ( 2009 ). Antarctic Yeasts: Biodiversity and Potential. . In Yeasts Biotechnology: Diversity and Applications, pp. 318. Edited by Satyanarayana T., Kunze G. . Berlin:: Springer Verlag;. [View Article]
    [Google Scholar]
  35. Simonov I. M. ( 1977 ). Physical-geographic description of the Fildes peninsula (South Shetland Islands). . Polar Geogr 1, 223242. [View Article]
    [Google Scholar]
  36. Song C., Chi Z., Li J., Wang X. ( 2010 ). β-Galactosidase production by the psychrotolerant yeast Guehomyces pullulans 17-1 isolated from sea sediment in Antarctica and lactose hydrolysis. . Bioprocess Biosyst Eng 33, 10251031. [View Article] [PubMed]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  38. Turchetti B., Buzzini P., Goretti M., Branda E., Diolaiuti G., D’Agata C., Smiraglia C., Vaughan-Martini A. ( 2008 ). Psychrophilic yeasts in glacial environments of Alpine glaciers. . FEMS Microbiol Ecol 63, 7383. [View Article] [PubMed]
    [Google Scholar]
  39. Vaca I., Faúndez C., Maza F., Paillavil B., Hernández V., Acosta F., Levicán G., Martínez C., Chávez R. ( 2013 ). Cultivable psychrotolerant yeasts associated with Antarctic marine sponges. . World J Microbiol Biotechnol 29, 183189. [View Article] [PubMed]
    [Google Scholar]
  40. Vishniac H. S. ( 2006 ). Yeast biodiversity in the Antarctic. . In Biodiversity and Ecophysiology of Yeasts, pp. 419440. Edited by Rosa C. A., Gábor P. . Berlin:: Springer-Verlag;. [View Article]
    [Google Scholar]
  41. White T. J., Bruns T., Lee S., Taylor J. ( 1990 ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols: a Guide to Methods and Applications, pp. 315322. Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. . San Diego, CA:: Academic Press;.
    [Google Scholar]
  42. Wirth F., Goldani L. Z. ( 2012 ). Epidemiology of Rhodotorula: an emerging pathogen. . Interdiscip Perspect Infect Dis 2012, 465717.[PubMed]
    [Google Scholar]
  43. Yarrow D. ( 1998 ). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 4th edn., pp. 77100. Edited by Kurtzman C. P., Fell J. W. . Amsterdam:: Elsevier;. [View Article]
    [Google Scholar]
  44. Zhao J. H., Bai F. Y., Guo L. D., Jia J. H. ( 2002 ). Rhodotorula pinicola sp. nov., a basidiomycetous yeast species isolated from xylem of pine twigs. . FEMS Yeast Res 2, 159163.[PubMed]
    [Google Scholar]
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