1887

Abstract

Two actinomycete strains isolated from marine sediment samples, designated YIM M11335 (from the Indian Ocean) and 12A09 (from the South China Sea), were obtained and examined by a polyphasic approach. The two Gram-staining-positive, aerobic strains produced branched substrate mycelia and aerial hyphae that were not fragmented, and no diffusible pigment was produced on the media tested. At maturity, spore chains and single spores were formed on aerial hyphae and substrate mycelium, respectively. Whole-organism hydrolysates of both strains contained -diaminopimelic acid and the diagnostic sugars glucose and galactose. Their predominant menaquinones were MK-10(H), MK-10(H), MK-11(H), MK-11(H) and MK-11(H) for strain YIM 11335 and MK-10(H), MK-10(H), MK-11(H), MK-11(H) and MK-11(H) for strain 12A09. The polar lipids detected in the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylcholine, an unknown phosphoglycolipid and several unknown glycolipids, phospholipids and polar lipids. The major fatty acids (>10 %) were iso-C and C for strain YIM 11335 and iso-C for strain 12A09. The GC contents of the genomic DNA of strains YIM 11335 and 12A09 were 70.7 % and 74.4 %, respectively. DNA–DNA hybridization relatedness values of these two isolates with the type strains DSM 45083 and YIM 91355 supported the hypothesis they are representatives of two different species. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the two isolates belong to the genus of the family and that the type strain of should be reclassified as a representative of comb. nov. The names proposed for the two novel species are sp. nov. (type strain YIM M11335 = DSM 45723 = CCTCC AB 2012051) and sp. nov. (type strain 12A09 = KCTC 29145 = CCTCC AB 2013140), respectively. An emended description of the genus is also proposed in the light of the new data.

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National High-tech R & D Program of China (Award 2012AA091606)
  • National Natural Science Foundation of China (Award 312700040 and 41006088)
  • West Light Foundation of the Chinese Academy of Sciences.
  • ‘Hundred Talents Program’ of the Chinese Academy of Sciences
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052704-0
2013-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4447.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052704-0&mimeType=html&fmt=ahah

References

  1. Athalye M., Goodfellow M., Lacey J., White R. P. ( 1985 ). Numerical classification of Actinomadura and Nocardiopsis . . Int J Syst Bacteriol 35, 8698. [View Article]
    [Google Scholar]
  2. Cai M., Zhi X.-Y., Tang S.-K., Zhang Y.-Q., Xu L.-H., Li W.-J. ( 2008 ). Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil. . Int J Syst Evol Microbiol 58, 15561560. [View Article] [PubMed]
    [Google Scholar]
  3. Cai M., Tang S.-K., Chen Y.-G., Li Y., Zhang Y.-Q., Li W.-J. ( 2009 ). Streptomonospora amylolytica sp. nov. and Streptomonospora flavalba sp. nov., two novel halophilic actinomycetes isolated from a salt lake. . Int J Syst Evol Microbiol 59, 24712475. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48, 459470. [View Article]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  6. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L. ( 2001 ). Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . . Int J Syst Evol Microbiol 51, 357363.[PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S.-L., Miura H., Matsui K., Yabuuchi E. ( 1988 ). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. . J Clin Microbiol 26, 17081713.[PubMed]
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  9. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  10. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  11. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  12. Goodfellow M. ( 1971 ). Numerical taxonomy of some nocardioform bacteria. . J Gen Microbiol 69, 3380. [View Article] [PubMed]
    [Google Scholar]
  13. Hiraishi A., Komagata K. ( 1989 ). Effects of the growth medium composition on the menaquinone homolog formation in Micrococcus luteus . . J Gen Appl Microbiol 35, 311318. [View Article]
    [Google Scholar]
  14. Hozzein W. N., Goodfellow M. ( 2008 ). Nocardiopsis arabia sp. nov., a halotolerant actinomycete isolated from a sand-dune soil. . Int J Syst Evol Microbiol 58, 25202524. [View Article] [PubMed]
    [Google Scholar]
  15. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141145.[PubMed]
    [Google Scholar]
  16. Kawato N., Shinobu R. ( 1959 ). On Streptomyces herbaricolor sp. nov. Supplement: a simple technique for the microscopic observation. . Mem Osaka Univ Lib Arts Educ B Nat Sci 8, 114119.
    [Google Scholar]
  17. Kelly K. L. ( 1964 ). Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. .
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  19. Li W.-J., Xu P., Zhang L.-P., Tang S.-K., Cui X.-L., Mao P.-H., Xu L.-H., Schumann P., Stackebrandt E., Jiang C.-L. ( 2003 ). Streptomonospora alba sp. nov., a novel halophilic actinomycete, and emended description of the genus Streptomonospora Cui et al. 2001. . Int J Syst Evol Microbiol 53, 14211425. [View Article] [PubMed]
    [Google Scholar]
  20. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China). . Int J Syst Evol Microbiol 57, 14241428. [View Article] [PubMed]
    [Google Scholar]
  21. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  23. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 8795. [View Article]
    [Google Scholar]
  24. Pridham T. G., Gottlieb D. ( 1948 ). The utilization of carbon compounds by some Actinomycetales as an aid for species determination. . J Bacteriol 56, 107114.[PubMed]
    [Google Scholar]
  25. Saddler G. S., Goodfellow M., Minnikin D. E., O’Donnell A. G. ( 1986 ). Influence of the growth cycle on the fatty acids and menaquinone composition of Streptomyces cyaneus NCIB 9616. . J Appl Bacteriol 60, 5156. [View Article]
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  27. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20, 16.
    [Google Scholar]
  28. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [View Article]
    [Google Scholar]
  29. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. ( 1997 ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47, 479491. [View Article]
    [Google Scholar]
  30. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  31. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. ( 1983 ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54, 3136. [View Article]
    [Google Scholar]
  32. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  33. Tang S.-K., Wang Y., Chen Y., Lou K., Cao L.-L., Xu L.-H., Li W.-J. ( 2009 ). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . . Int J Syst Evol Microbiol 59, 20252031. [View Article] [PubMed]
    [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  36. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 11491153. [View Article] [PubMed]
    [Google Scholar]
  37. Zhi X.-Y., Tang S.-K., Li W.-J., Xu L.-H., Jiang C.-L. ( 2006 ). New genus-specific primers for the PCR identification of novel isolates of the genus Streptomonospora . . FEMS Microbiol Lett 263, 4853. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052704-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052704-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error