1887

Abstract

A Gram-reaction-negative, yellow-pigmented strain, designated EX36, was characterized using a polyphasic approach comprising phylogenetic, morphological and genotypic analyses. The endophytic strain was isolated from Zn/Cd-accumulating in Arnoldstein, Austria. Analysis of the 16S rRNA gene demonstrated that the novel strain is most closely related to members of the genus (95 % sequence similarity with ). The genomic DNA G+C content was 47.2 mol%. The predominant quinone was and the major cellular fatty acids were summed feature 3 (iso-C 2-OH and/or Cω7), Cω5, iso-C 3-OH and iso-C. On the basis of its phenotypic and genotypic properties, strain EX36 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EX36 ( = DSM 26130 = LMG 27272).

Funding
This study was supported by the:
  • Austrian Science Foundation (Award L561-B17)
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2013-12-01
2022-01-28
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References

  1. Baik K. S., Kim M. S., Park S. C., Lee D. W., Lee S. D., Ka J.-O., Choi S. K., Seong C. N. ( 2007 ). Spirosoma rigui sp. nov., isolated from fresh water. . Int J Syst Evol Microbiol 57, 28702873. [View Article] [PubMed]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  3. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992993.[PubMed]
    [Google Scholar]
  4. Denner E. B. M., Paukner S., Kämpfer P., Moore E. R. B., Abraham W. R., Busse H.-J., Wanner G., Lübitz W. ( 2001 ). Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan. . Int J Syst Evol Microbiol 51, 827841. [View Article] [PubMed]
    [Google Scholar]
  5. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C. ( 1989 ). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. . Nucleic Acids Res 17, 78437853. [View Article] [PubMed]
    [Google Scholar]
  6. Finster K. W., Herbert R. A., Lomstein B. A. ( 2009 ). Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma . . Int J Syst Evol Microbiol 59, 839844. [View Article] [PubMed]
    [Google Scholar]
  7. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Kuffner M., Puschenreiter M., Wieshammer G., Gorfer M., Sessitsch A. ( 2008 ). Rhizosphere bacteria affect growth and metal uptake of heavy metal accumulating willows. . Plant Soil 304, 3544. [View Article]
    [Google Scholar]
  10. Kuffner M., De Maria S., Puschenreiter M., Fallmann K., Wieshammer G., Gorfer M., Strauss J., Rivelli A. R., Sessitsch A. ( 2010 ). Culturable bacteria from Zn- and Cd-accumulating Salix caprea with differential effects on plant growth and heavy metal availability. . J Appl Microbiol 108, 14711484. [View Article] [PubMed]
    [Google Scholar]
  11. Larkin J. M., Borrall R. ( 1984 ). Family I. Spirosomaceae Larkin and Borrall 1978, 595AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 125126. Edited by Krieg N. R., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  13. Myers E. W., Miller W. ( 1988 ). Optimal alignments in linear space. . Comput Appl Biosci 4, 1117.[PubMed]
    [Google Scholar]
  14. Neidhardt F. C., Bloch P. L., Smith D. F. ( 1974 ). Culture medium for enterobacteria. . J Bacteriol 119, 736747.[PubMed]
    [Google Scholar]
  15. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35, 71887196. [View Article] [PubMed]
    [Google Scholar]
  16. Silvestro D., Michalak I. ( 2012 ). raxmlGUI: A graphical front-end for RAxML. . Org Divers Evol 12, 335337. [View Article]
    [Google Scholar]
  17. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Stamatakis A. ( 2006a ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  19. Stamatakis A. ( 2006b ). Phylogenetic models of rate heterogeneity: a high performance computing perspective. . In: Proc. of IPDPS2006, pp. 253. IEEE Computer Society;. Washington, DC:.
    [Google Scholar]
  20. Stamatakis A., Hoover P., Rougemont J. ( 2008 ). A rapid bootstrap algorithm for the RAxML Web servers. . Syst Biol 57, 758771. [View Article] [PubMed]
    [Google Scholar]
  21. Ten L. N., Xu J.-L., Jin F.-X., Im W.-T., Oh H.-M., Lee S.-T. ( 2009 ). Spirosoma panaciterrae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59, 331335. [View Article] [PubMed]
    [Google Scholar]
  22. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  23. Yang Z. H. ( 1994 ). Maximum likelihood phylogenetic estimation from DNA sequences with variable rates over sites: approximate methods. . J Mol Evol 39, 306314. [View Article] [PubMed]
    [Google Scholar]
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