1887

Abstract

A Gram-reaction-negative, yellow-pigmented strain, designated EX36, was characterized using a polyphasic approach comprising phylogenetic, morphological and genotypic analyses. The endophytic strain was isolated from Zn/Cd-accumulating in Arnoldstein, Austria. Analysis of the 16S rRNA gene demonstrated that the novel strain is most closely related to members of the genus (95 % sequence similarity with ). The genomic DNA G+C content was 47.2 mol%. The predominant quinone was and the major cellular fatty acids were summed feature 3 (iso-C 2-OH and/or Cω7), Cω5, iso-C 3-OH and iso-C. On the basis of its phenotypic and genotypic properties, strain EX36 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EX36 ( = DSM 26130 = LMG 27272).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052654-0
2013-12-01
2020-01-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4586.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052654-0&mimeType=html&fmt=ahah

References

  1. Baik K. S., Kim M. S., Park S. C., Lee D. W., Lee S. D., Ka J.-O., Choi S. K., Seong C. N.. ( 2007;). Spirosoma rigui sp. nov., isolated from fresh water. . Int J Syst Evol Microbiol 57:, 2870–2873. [CrossRef][PubMed]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  3. Buck J. D.. ( 1982;). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  4. Denner E. B. M., Paukner S., Kämpfer P., Moore E. R. B., Abraham W. R., Busse H.-J., Wanner G., Lübitz W.. ( 2001;). Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan. . Int J Syst Evol Microbiol 51:, 827–841. [CrossRef][PubMed]
    [Google Scholar]
  5. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C.. ( 1989;). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. . Nucleic Acids Res 17:, 7843–7853. [CrossRef][PubMed]
    [Google Scholar]
  6. Finster K. W., Herbert R. A., Lomstein B. A.. ( 2009;). Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma. . Int J Syst Evol Microbiol 59:, 839–844. [CrossRef][PubMed]
    [Google Scholar]
  7. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  9. Kuffner M., Puschenreiter M., Wieshammer G., Gorfer M., Sessitsch A.. ( 2008;). Rhizosphere bacteria affect growth and metal uptake of heavy metal accumulating willows. . Plant Soil 304:, 35–44. [CrossRef]
    [Google Scholar]
  10. Kuffner M., De Maria S., Puschenreiter M., Fallmann K., Wieshammer G., Gorfer M., Strauss J., Rivelli A. R., Sessitsch A.. ( 2010;). Culturable bacteria from Zn- and Cd-accumulating Salix caprea with differential effects on plant growth and heavy metal availability. . J Appl Microbiol 108:, 1471–1484. [CrossRef][PubMed]
    [Google Scholar]
  11. Larkin J. M., Borrall R.. ( 1984;). Family I. Spirosomaceae Larkin and Borrall 1978, 595AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 125–126. Edited by Krieg N. R., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  13. Myers E. W., Miller W.. ( 1988;). Optimal alignments in linear space. . Comput Appl Biosci 4:, 11–17.[PubMed]
    [Google Scholar]
  14. Neidhardt F. C., Bloch P. L., Smith D. F.. ( 1974;). Culture medium for enterobacteria. . J Bacteriol 119:, 736–747.[PubMed]
    [Google Scholar]
  15. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O.. ( 2007;). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. . Nucleic Acids Res 35:, 7188–7196. [CrossRef][PubMed]
    [Google Scholar]
  16. Silvestro D., Michalak I.. ( 2012;). raxmlGUI: A graphical front-end for RAxML. . Org Divers Evol 12:, 335–337. [CrossRef]
    [Google Scholar]
  17. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Stamatakis A.. ( 2006a;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  19. Stamatakis A.. ( 2006b;). Phylogenetic models of rate heterogeneity: a high performance computing perspective. . In: Proc. of IPDPS2006, pp. 253. IEEE Computer Society;. Washington, DC:.
    [Google Scholar]
  20. Stamatakis A., Hoover P., Rougemont J.. ( 2008;). A rapid bootstrap algorithm for the RAxML Web servers. . Syst Biol 57:, 758–771. [CrossRef][PubMed]
    [Google Scholar]
  21. Ten L. N., Xu J.-L., Jin F.-X., Im W.-T., Oh H.-M., Lee S.-T.. ( 2009;). Spirosoma panaciterrae sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59:, 331–335. [CrossRef][PubMed]
    [Google Scholar]
  22. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  23. Yang Z. H.. ( 1994;). Maximum likelihood phylogenetic estimation from DNA sequences with variable rates over sites: approximate methods. . J Mol Evol 39:, 306–314. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052654-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052654-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error