1887

Abstract

An actinobacterial strain was isolated from a sediment sample from Chorao Island, in Goa province, India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021, was a Gram-stain positive, aerobic, non-motile and coccoid. Strain NIO-1021 was identified as a member of the genus by a polyphasic approach. Strain NIO-1021 could be differentiated from other members of the genus on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021 to the most closely related species, KCTC 9943, was 98.6 % with 19 nt differences). Furthermore, DNA–DNA hybridization analysis revealed that the novel strain had lower relatedness with the type strains of other members of the genus . The strain formed a monophyletic clade with with 100 % bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H). The major fatty acids were anteiso-C, iso-C and anteiso-C. The DNA GC content of strain NIO-1021 was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification as representing a member of the genus . On the basis of phenotypic, chemotypic and molecular characteristics, strain NIO-1021 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed, with strain NIO-1021 ( = NCIM 5455 = DSM 25126 = CCTCC AA 209050) as the type strain.

Funding
This study was supported by the:
  • , Council for Scientific and Industrial Research (CSIR)

Erratum

This article contains a correction applying to the following content:
Corrigendum: sp. nov., isolated from a sediment sample
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2014-03-01
2020-09-25
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