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Volume 64, Issue Pt_5

sp. nov., isolated from lake trout () and brown trout (), and emended descriptions of and Free

Abstract

Two strains (T68 and T62) of a Gram-reaction-negative, yellow-pigmented bacterium containing flexirubin-type pigments were recovered from the kidney of a cultured lake trout () and necrotic fins of a brown trout () during disease surveillance in 2009. Both isolates possessed catalase and cytochrome oxidase activities and degraded multiple substrates (e.g. gelatin, casein, elastin and Tweens 20 and 80). The mean DNA G+C content of strain T68 was 34.1 mol%. 16S rRNA gene sequencing demonstrated that strains T68 and T62 had nearly identical sequences (≥99 % similarity) and placed the bacterium within the genus , where THG 15 (97.8 %), DSM 19109 (97.7 %) and PSD1-4 (97.6 %) were its closest relatives. Subsequent phylogenetic analyses using neighbour-joining, maximum-parsimony and Bayesian methodologies demonstrated that strains T68 and T62 formed a well-supported clade (bootstrap values of 100 and 97 %; posterior probability 0.99) that was distinct from other species of the genus . The major fatty acids of strains T68 and T62 were characteristic of the genus and included iso-C, summed feature 3 (Cω6 and/or Cω7), iso-C 3-OH, C and C 3-OH. The mean DNA–DNA relatedness of strain T68 to JCM 16719 and LMG 24952 was 24 and 21 %, respectively. Based on the results from our polyphasic characterization, strains T68 and T62 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T68 ( = LMG 27338 = ATCC BAA-2540). Emended descriptions of and are also proposed.

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Funding
This study was supported by the:
  • Great Lakes Fishery Trust (Lansing, MI) (Award 2010-1147)
© 2014 IUMS
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2014-05-01
2024-04-18
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Chryseobacteriumaahli sp. nov., isolated from lake trout (Salvelinus namaycush) and brown trout (Salmo trutta), and emended descriptions of Chryseobacterium ginsenosidimutans and Chryseobacterium gregarium
Int J Syst Evol Microbiol 64, 1573 (2014); https://doi.org/10.1099/ijs.0.052373-0
/content/journal/ijsem/10.1099/ijs.0.052373-0
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