1887

Abstract

A Gram-staining-negative, non-motile, spore-forming, rod-shaped, marine bacterial strain, CL-KR2, was isolated from tropical seawater near Kosrae, an island in the Federated States of Micronesia. Analysis of the 16S rRNA gene sequence of strain CL-KR2 revealed a clear affiliation with the genus . Based on phylogenetic analysis, strain CL-KR2 showed the closest phylogenetic relationship to CL-CB462, with 16S rRNA gene sequence similarity of 96.6 %. DNA–DNA relatedness between strain CL-KR2 and CL-CB462 was 6.7 % (reciprocal 9.5 %). Strain CL-KR2 grew in the presence of 1–20 % sea salts and the optimal salt concentration was 3.5–5 %. The temperature and pH optima for growth were 35 °C and pH 7.5. The major cellular fatty acids (≥10.0 %) of strain CL-KR2 were iso-C, summed feature 3 (iso-C 2-OH and/or Cω7) and iso-Cω9 and the only isoprenoid quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, two unidentified glycolipids and two unidentified lipids. The genomic DNA G+C content of strain CL-KR2 was 43.2 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-KR2 could be distinguished from the only member of the genus with a validly published name. Thus, strain CL-KR2 should be assigned to a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CL-KR2 ( = KCCM 90206 = JCM 18898).

Funding
This study was supported by the:
  • , National Research Foundation of Korea (NRF) , (Award 2011-0012369)
  • , Korea Institute of Ocean Science and Technology (KIOST) , (Award PE98962)
  • , East Asian Seas Time series-I (EAST-I)
  • , Ministry of Oceans and Fisheries, Korea
  • , BK21 project of the Korean Government
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2013-11-01
2020-11-30
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