Gram-negative, rod-shaped, oxidase-negative, facultatively anaerobic, yellow–orange-pigmented and motile bacterial strains, designated 8N4T, 9N2 and 10N3, were isolated from flower nectar of Amygdalus communis (almond) and Citrus paradisi (grapefruit). The 16S rRNA gene sequences of the strains shared highest sequence similarity of 97.0 % with that of Phaseolibacter flectens ATCC 12775T and lower similarity with sequences from other type strains of genera of the Enterobacteriaceae. A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA, gyrB, rpoB and atpD gene sequences supported the classification of strains 8N4T, 9N2 and 10N3 within a novel species in a novel genus in the family Enterobacteriaceae. Strain 8N4T, and the reference strains of the novel species, grew at 4–35 °C (optimum, 28–30 °C), with 0–5.0 % NaCl (optimum, 3 % NaCl) and with 0–60 % sucrose (optimum, 10–25 % sucrose). Their major cellular fatty acids were C16 : 0, C17 : 0 cyclo, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain 8N4T was 46.8 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the floral nectar isolates are classified within a novel species in a new genus in the family Enterobacteriaceae, for which the name Rosenbergiella nectarea gen. nov., sp. nov. is proposed. The type strain of Rosenbergiella nectarea is 8N4T ( = LMG 26121T = DSM 24150T).
Published Online:
Funding
This study was supported by the:
Israel Science Foundation (ISF)
(Award 1094/12 and 189/08)
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res25, 3389–3402. [View Article][PubMed]
Ben-Ze’evI. S.,
LevyE.,
EilamT.,
AniksterY.(2005). Whole-cell fatty acid profiles – a tool for species and subspecies classification in the Puccinia recondita complex. . J Plant Pathol87, 187–197.
BradyC. L.,
CleenwerckI.,
VenterS. N.,
VancanneytM.,
SwingsJ.,
CoutinhoT.(2008). Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). . Syst Appl Microbiol31, 447–460. [View Article][PubMed]
BradyC. L.,
VenterS. N.,
CleenwerckI.,
VandemeulebroeckeK.,
De VosP.,
CoutinhoT. A.(2010). Transfer of Pantoea citrea, Pantoea punctata and Pantoea terrea to the genus Tatumella emend. as Tatumella citrea comb. nov., Tatumella punctata comb. nov. and Tatumella terrea comb. nov. and description of Tatumella morbirosei sp. nov.. Int J Syst Evol Microbiol60, 484–494. [View Article][PubMed]
BrennerD. J.,
FarmerJ. J.III(2005). Family V. Enterobacteriaceae
. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2B, pp. 587–848. Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. R.,
GarrityG. M.
. New York:: Springer;.
DrancourtM.,
BolletC.,
CartaA.,
RousselierP.(2001). Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov.. Int J Syst Evol Microbiol51, 925–932. [View Article][PubMed]
GaviniF.,
MergaertJ.,
BejiA.,
MielcarekC.,
IzardD.,
KerstersK.,
De LeyJ.(1989). Transfer of Enterobacter agglomerans (Beijerinck 1988) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov.. Int J Syst Bacteriol39, 337–345. [View Article]
GrimontF.,
GrimontP. A. D.(2006). The genus Serratia
. . In The Prokaryotes, , 3rd edn., vol. 6, pp. 219–244. Edited by
DworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK. H.,
StackebrandtE.
. New York:: Springer;. [View Article]
GrimontP. A. D.,
GrimontF.,
FarmerJ. J.III,
AsburyM. A.(1981).Cedecea davisae gen. nov., sp. nov. and Cedecea lapagei sp. nov., new Enterobacteriaceae from clinical specimens. . Int J Syst Bacteriol31, 317–326. [View Article]
HalpernM.,
FridmanS.,
Aizenberg-GershteinY.,
IzhakiI.(2013). Transfer of Pseudomonas flectens Johnson 1956 to Phaseolibacter gen. nov., in the family Enterobacteriaceae, as Phaseolibacter flectens gen. nov., comb. nov.. Int J Syst Evol Microbiol63, 268–273. [View Article][PubMed]
HollisD. G.,
HickmanF. W.,
FanningG. R.,
FarmerJ. J.III,
WeaverR. E.,
BrennerD. J.(1981).Tatumella ptyseos gen. nov., sp. nov., a member of the family Enterobacteriaceae found in clinical specimens. . J Clin Microbiol14, 79–88.[PubMed]
IversenC.,
MullaneN.,
McCardellB.,
TallB. D.,
LehnerA.,
FanningS.,
StephanR.,
JoostenH.(2008).Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.. Int J Syst Evol Microbiol58, 1442–1447. [View Article][PubMed]
KämpferP.,
RuppelS.,
RemusR.(2005).Enterobacter radicincitans sp. nov., a plant growth promoting species of the family Enterobacteriaceae
. . Syst Appl Microbiol28, 213–221. [View Article][PubMed]
MergaertJ.,
VerdonckL.,
KerstersK.(1993). Transfer of Erwinia ananas (synonym, Erwinia uredovora) and Erwinia stewartii to the genus Pantoea emend. as Pantoea ananas (Serrano 1928) comb. nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoea stewartii subsp. indologenes subsp. nov.. Int J Syst Bacteriol43, 162–173. [View Article]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
RaatsD.,
HalpernM.(2007).Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. . Int J Syst Evol Microbiol57, 255–259. [View Article][PubMed]
WilsonK.(1987). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
. New York:: Greene Publishing and Wiley-Interscience;.