1887

Abstract

Two bacterial strains (JC130 and JA747) were isolated from dry soils of a solar saltern. Phylogenetic analysis showed that strain JA747 clustered with species of the genus belonging to the family of the class in the phylum , while strain JC130 clustered with species of the genus belonging to the family of the phylum . Based on 16S rRNA gene sequence analysis, strain JA747 had highest similarity with K7-2 (96.7 %) and other members of the genus (<96 %). Strain JC130 had highest sequence similarity with X14-1 (98.1 %), KMM 6156 (96.9 %) and other members of the genus (<96 %). However, strain JC130 showed less than 32 % DNA reassociation value (based on DNA–DNA hybridization) with NRRL B-51097 ( = X14-1) and LMG 23027 ( = KMM 6156). Strain JA747 was positive for catalase and oxidase activity and negative for nitrate reduction, and hydrolysis of starch and casein. Phosphatidylglycerol, diphostatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids for strain JA747. Cω7, Cω6/Cω7, Cω6 and C were the major fatty acids of strain JA747. Strain JC130 was positive for catalase and oxidase, and negative for gelatinase and nitrate reduction. Phosphatidylethanolamine was the major polar lipid of strain JC130. Major fatty acids of strain JC130 were iso-C and summed feature 4 (anteiso-CB/iso I). Based on the phenotypic, chemotaxonomic and molecular evidence presented, strains JA747 and JC130 are considered to represent two novel species of the genera and , for which the names sp. nov. (type strain JA747 = KCTC 23981 = NBRC 108930) and sp. nov. (type strain JC130 = KCTC 23982 = LMG 26962), respectively, are proposed. Emended descriptions of the genera and are provided.

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2013-12-01
2019-10-19
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Bhadra B. , Roy P. , Chakraborty R. . ( 2005; ). Serratia ureilytica sp. nov., a novel urea-utilizing species. . Int J Syst Evol Microbiol 55:, 2155–2158. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cappuccino J. G. , Sherman N. . ( 1998; ). Microbiology – A Laboratory Manual, , 5th edn.. San Francisco:: Benjamin/Cummings Science Publishing;.
    [Google Scholar]
  4. Hiraishi A. , Hoshino Y. . ( 1984; ). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. . J Gen Appl Microbiol 30:, 435–448. [CrossRef]
    [Google Scholar]
  5. Hiraishi A. , Hoshino Y. , Kitamura H. . ( 1984; ). Isoprenoid quinone composition in the classification of Rhodospirillaceae . . J Gen Appl Microbiol 30:, 197–210. [CrossRef]
    [Google Scholar]
  6. Imhoff J. F. . ( 1984; ). Quinones of phototrophic purple bacteria. . FEMS Microbiol Lett 25:, 85–89. [CrossRef]
    [Google Scholar]
  7. Kalyan Chakravarthy S. , Srinivas T. N. R. , Anil Kumar P. , Sasikala Ch. , Ramana Ch. V. . ( 2007; ). Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov.. Int J Syst Evol Microbiol 57:, 2453–2457. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kates M. . ( 1972; ). Techniques of Lipidology. New York:: Elsevier;.
    [Google Scholar]
  9. Kates M. . ( 1986; ). Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids. Amsterdam:: Elsevier;.
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kumar P. A. , Srinivas T. N. R. , Sasikala Ch. , Ramana Ch. V. . ( 2007; ). Halochromatium roseum sp. nov., a non-motile phototrophic gammaproteobacterium with gas vesicles, and emended description of the genus Halochromatium . . Int J Syst Evol Microbiol 57:, 2110–2113. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  14. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Evol Microbiol 39:, 159–167.
    [Google Scholar]
  15. Nedashkovskaya O. I. , Kim S. B. , Suzuki M. , Shevchenko L. S. , Lee M. S. , Lee K. H. , Park M. S. , Frolova G. M. , Oh H. W. . & other authors ( 2005; ). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55:, 2583–2588. [CrossRef] [PubMed]
    [Google Scholar]
  16. Oren A. , Duker S. , Ritter S. . ( 1996; ). The polar lipid composition of Walsby’s square bacterium. . FEMS Microbiol Lett 138:, 135–140. [CrossRef]
    [Google Scholar]
  17. Raj P. S. , Ramaprasad E. V. V. , Vaseef S. , Sasikala Ch. , Ramana Ch. V. . ( 2013; ). Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. . Int J Syst Evol Microbiol 63:, 181–186. [CrossRef] [PubMed]
    [Google Scholar]
  18. Ritika C. , Suresh K. , Kumar P. A. . ( 2012; ). Caenispirillum salinarum sp. nov., a member of the family Rhodospirillaceae isolated from a solar saltern. . Int J Syst Evol Microbiol 62:, 1698–1702. [CrossRef] [PubMed]
    [Google Scholar]
  19. Rohmer M. , Bouvier-Nave P. , Ourisson G. . ( 1984; ). Distribution of hopanoid triterpenes in prokaryotes. . J Gen Microbiol 130:, 1137–1150.
    [Google Scholar]
  20. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
    [Google Scholar]
  21. Seldin L. , Dubnau D. . ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. . Int J Syst Bacteriol 35:, 151–154. [CrossRef]
    [Google Scholar]
  22. Shiba T. , Simidu U. . ( 1982; ). Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. . Int J Syst Bacteriol 32:, 211–217. [CrossRef]
    [Google Scholar]
  23. Srinivas T. N. R. , Kumar P. A. , Sasikala Ch. , Ramana Ch. V. , Imhoff J. F. . ( 2007; ). Rhodobium gokarnense sp. nov., a novel phototrophic alphaproteobacterium from a saltern. . Int J Syst Evol Microbiol 57:, 932–935. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tapilatu Y. H. , Grossi V. , Acquaviva M. , Militon C. , Bertrand J. C. , Cuny P. . ( 2010; ). Isolation of hydrocarbon-degrading extremely halophilic archaea from an uncontaminated hypersaline pond (Camargue, France). . Extremophiles 14:, 225–231. [CrossRef] [PubMed]
    [Google Scholar]
  26. Tindall B. J. . ( 1990a; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  27. Tindall B. J. . ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  28. Tourova T. P. , Antonov A. S. . ( 1988; ). Identification of microorganisms by rapid DNA-DNA hybridization. . Methods Microbiol 19:, 333–355. [CrossRef]
    [Google Scholar]
  29. Trüper H. G. , Pfennig N. . ( 1981; ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation and Identification of Bacteria, pp. 279–289. Edited by Starr M. P. , Stolp H. , Trüper H. G. , Balows A. , Schlegel H. G. . . Berlin:: Springer;.
    [Google Scholar]
  30. Venkata Ramana V. , Sasikala C. , Veera Venkata Ramaprasad E. , Venkata Ramana C. . ( 2010; ). Description of Ectothiorhodospira salini sp. nov.. J Gen Appl Microbiol 56:, 313–319. [CrossRef] [PubMed]
    [Google Scholar]
  31. Xu M. , Xin Y. , Yu Y. , Zhang J. , Zhou Y. , Liu H. , Tian J. , Li Y. . ( 2010; ). Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. . Int J Syst Evol Microbiol 60:, 2215–2220. [CrossRef] [PubMed]
    [Google Scholar]
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