1887

Abstract

A novel filamentous bacterium, strain SCSIO 11153, was isolated from a sediment sample collected from the Indian Ocean (80° 03.099′ E 01° 03.300′ N) at a depth of 4593 m. Good growth was observed at 50–55 °C and pH 7.0 with 3 % NaCl. It formed ivory–white colonies with radial wrinkles. Aerial mycelium was absent on the media tested. Phenotypic characteristics and 16S rRNA gene sequence analysis indicated that strain SCSIO 11153 belonged to the family . It exhibited 96.4 % and 96.2 % 16S rRNA gene sequence similarities to NariEX and Nari11A, respectively, while lower sequence similarity values (<95.4 %) were observed between strain SCSIO 11153 and other species of genera in the family . The menaquinone type was MK-7. Major cellular fatty acids were iso-C, anteiso-C and iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain SCSIO 11153 was 52.6 mol%. On the basis of the genotypic and phenotypic characteristics, it is proposed that strain SCSIO 11153 represents a novel species of the genus with the name sp. nov. The type strain is SCSIO 11153 ( = DSM 45787 = CCTCC AA 2012007 = NBRC 109068).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052175-0
2013-12-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4552.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052175-0&mimeType=html&fmt=ahah

References

  1. Addou A. N., Schumann P., Spröer C., Hacene H., Cayol J. L., Fardeau M. L.. ( 2012;). Melghirimyces algeriensis gen. nov., sp. nov., a member of the family Thermoactinomycetaceae, isolated from a salt lake. . Int J Syst Evol Microbiol 62:, 1491–1498. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Hatayama K., Shoun H., Ueda Y., Nakamura A.. ( 2005;). Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family ‘Thermoactinomycetaceae’ isolated from compost. . Int J Syst Evol Microbiol 55:, 2101–2104. [CrossRef][PubMed]
    [Google Scholar]
  7. Kelly K. L.. ( 1964;). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  9. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  10. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  11. Li J., Zhang G. T., Yang J., Tian X. P., Wang F. Z., Zhang C. S., Zhang S., Li W. J.. ( 2012;). Marininema mesophilum gen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae. . Int J Syst Evol Microbiol 62:, 1383–1388. [CrossRef][PubMed]
    [Google Scholar]
  12. Matsuo Y., Katsuta A., Matsuda S., Shizuri Y., Yokota A., Kasai H.. ( 2006;). Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov.. Int J Syst Evol Microbiol 56:, 2837–2842. [CrossRef][PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  15. Park D. J., Dastager S. G., Lee J. C., Yeo S. H., Yoon J. H., Kim C. J.. ( 2007;). Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae. . Int J Syst Evol Microbiol 57:, 2660–2664. [CrossRef][PubMed]
    [Google Scholar]
  16. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  18. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  19. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  22. Tsubouchi T., Shimane Y., Mori K., Usui K., Hiraki T., Tame A., Uematsu K., Maruyama T., Hatada Y.. ( 2013;). Polycladomyces abyssicola gen. nov., sp. nov., a thermophilic filamentous bacterium isolated from hemipelagic sediment. . Int J Syst Evol Microbiol 63:, 1972–1981. [CrossRef][PubMed]
    [Google Scholar]
  23. von Jan M., Riegger N., Pötter G., Schumann P., Verbarg S., Spröer C., Rohde M., Lauer B., Labeda D. P., Klenk H.-P.. ( 2011;). Kroppenstedtia eburnea gen. nov., sp. nov., a thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009. . Int J Syst Evol Microbiol 61:, 2304–2310. [CrossRef][PubMed]
    [Google Scholar]
  24. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  25. Yassin A. F., Hupfer H., Klenk H.-P., Siering C.. ( 2009;). Desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006. . Int J Syst Evol Microbiol 59:, 454–459. [CrossRef][PubMed]
    [Google Scholar]
  26. Yoon J. H., Kim I. G., Shin Y. K., Park Y. H.. ( 2005;). Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. . Int J Syst Evol Microbiol 55:, 395–400. [CrossRef][PubMed]
    [Google Scholar]
  27. Yu T. T., Zhang B. H., Yao J. C., Tang S. K., Zhou E. M., Yin Y. R., Wei D. Q., Ming H., Li W. J.. ( 2012;). Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China. . Antonie van Leeuwenhoek 102:, 711–718. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052175-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052175-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error