sp. nov., a novel thermoactinomycete isolated from a sediment sample, and emended description of the genus Li 2012 Free

Abstract

A novel Gram-stain-positive bacterium, designated strain YIM M11385, was isolated from a marine sediment sample collected from the South Bay, Little Andaman Island, India with a salinity of 35 p.p.m., pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M11385 belongs to the genus , supported by a bootstrap value of 100 %. The taxonomic position of this organism was further established by using a polyphasic approach. Strain YIM M11385 grew optimally at 28 °C, pH 7.0 and in the presence of 0–5 % (w/v) NaCl. The 16S rRNA gene sequence similarity between strain YIM M11385 and SCSIO 10219 was 98.3 %. Strain YIM M11385 exhibited a quinone system with only MK-7, the polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major components, and the major fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. The level of DNA–DNA relatedness between strain YIM M11385 and SCSIO 10219 was 59.36 %. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M11385 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM M11385 ( = CCTCC AB 2012052 = DSM 45789). In addition, we propose that the description of the genus should be further emended based on the results of the present study.

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • Chinese Academy of Sciences
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2013-12-01
2024-03-29
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