1887

Abstract

A novel actinomycete strain, designed MW4-36, was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus . The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and -diaminopimelic acid including 3-hydroxy--diaminopimelic acid; arabinose, glucose, rhamnose, ribose and xylose were found as whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; no phosphatidylcholine was found. The predominant menaquinones were MK-9(H) and MK-9(H). Mycolic acids were not detected. The predominant cellular fatty acids were iso-C, iso-C, anteiso-C and anteiso-C. The G+C content of the genomic DNA was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that the strain should be classified in the genus and showed that the closest relative was JCM 15933 (99.4 % similarity). These taxonomic data revealed that strain MW4-36 could be readily distinguished from its phylogenetically closest relative. On the basis of these phenotypic and genotypic data, strain MW4-36 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is MW4-36 ( = BCC 34901 = NBRC 106093).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052092-0
2013-11-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4033.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052092-0&mimeType=html&fmt=ahah

References

  1. Arai T. . ( 1975; ). Culture Media for Actinomycetes. Tokyo:: Society for Actinomycetes Japan;.
    [Google Scholar]
  2. Chiaraphongphon S. , Suriyachadkun C. , Tamura T. , Thawai C. . ( 2010; ). Dactylosporangium maewongense sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60:, 1200–1205. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  4. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  5. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H. N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  9. Hayakawa M. , Sadakata T. , Kajiura T. , Nonomura H. . ( 1991; ). New methods for the highly selective isolation of Micromonospora and Microbispora from soil. . J Ferment Bioeng 72:, 320–326. [CrossRef]
    [Google Scholar]
  10. Itoh T. , Kudo T. , Parenti F. , Seino A. . ( 1989; ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. . Int J Syst Bacteriol 39:, 168–173. [CrossRef]
    [Google Scholar]
  11. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  12. Kawamoto I. , Oka T. , Nara T. . ( 1981; ). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146:, 527–534.[PubMed]
    [Google Scholar]
  13. Kim B. Y. , Stach J. E. M. , Weon H. Y. , Kwon S. W. , Goodfellow M. . ( 2010; ). Three new species of Dactylosporangium isolated from soil: Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov.. Int J Syst Evol Microbiol 60:, 1813–1823.[PubMed] [CrossRef]
    [Google Scholar]
  14. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  16. Komagata K. , Suzuki K. I. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  17. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–148. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  18. Lechevalier M. P. , Lechevalier H. A. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  19. Lechevalier M. P. , Lechevalier H. A. . ( 1980; ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 227–291. Edited by Dietz A. , Thayer D. W. . . Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  20. Lechevalier M. P. , DeBievre C. , Lechevalier H. A. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E. , Alshamaony L. , Goodfellow M. . ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88:, 200–204. [CrossRef] [PubMed]
    [Google Scholar]
  22. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  23. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  24. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  25. Seo S. H. , Lee S. D. . ( 2010; ). Dactylosporangium darangshiense sp. nov., isolated from rock soil. . Int J Syst Evol Microbiol 60:, 1256–1260. [CrossRef] [PubMed]
    [Google Scholar]
  26. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  27. Shomura T. , Kojima M. , Yoshida J. , Ito M. , Amano S. , Totsugawa K. , Niwa T. , Inouye S. , Ito T. , Niida T. . ( 1980; ). Studies on a new aminoglycoside antibiotic, dactimicin. I. Producing organism and fermentation. . J Antibiot (Tokyo) 33:, 924–930. [CrossRef] [PubMed]
    [Google Scholar]
  28. Shomura T. , Yoshida J. , Miyadoh S. , Ito T. , Niida T. . ( 1983; ). Dactylosporangium vinaceum sp. nov.. Int J Syst Bacteriol 33:, 309–313. [CrossRef]
    [Google Scholar]
  29. Shomura T. , Amano S. , Tohyama H. , Yoshida J. , Ito T. , Niida T. . ( 1985; ). Dactylosporangium roseum sp. nov.. Int J Syst Bacteriol 35:, 1–4. [CrossRef]
    [Google Scholar]
  30. Shomura T. , Amano S. , Yoshida J. , Kojima M. . ( 1986; ). Dactylosporangium fulvum sp. nov.. Int J Syst Bacteriol 36:, 166–169. [CrossRef]
    [Google Scholar]
  31. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  32. Suriyachadkun C. , Chunhametha S. , Thawai C. , Tamura T. , Potacharoen W. , Kirtikara K. , Sanglier J. J. . ( 2009; ). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. . Int J Syst Evol Microbiol 59:, 992–997. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tamaoka J. . ( 1994; ). Determination of DNA base composition. . In Chemical Methods in Prokaryotic Systematics, pp. 463–470. Edited by Goodfellow M. , O’Donnell A. G. . . Chichester:: Wiley;.
    [Google Scholar]
  34. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  35. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  36. Thawai C. , Tanasupawat S. , Kudo T. . ( 2011; ). Dactylosporangium tropicum sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61:, 2358–2362. [CrossRef] [PubMed]
    [Google Scholar]
  37. Thiemann J. E. . ( 1970; ). Dactylosporangium thailandensis should be D. thailandense . . Int J Syst Bacteriol 20:, 59. [CrossRef]
    [Google Scholar]
  38. Thiemann J. E. , Pagani H. , Beretta G. . ( 1967; ). A new genus of the Actinoplanaceae: Dactylosporanguim, gen. nov. . Arch Mikrobiol 58:, 42–52. [CrossRef] [PubMed]
    [Google Scholar]
  39. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  40. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  41. Uchida K. , Aida K. . ( 1984; ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol 30:, 131–134. [CrossRef]
    [Google Scholar]
  42. Verlander C. P. . ( 1992; ). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185–201. Edited by Kricka L. J. . . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  43. Williams S. T. , Cross T. . ( 1971; ). Actinomycetes. . In Methods in Microbiology, vol. 4, pp. 295–334. Edited by Booth C. . . London:: Academic Press;.
    [Google Scholar]
  44. Zhi X. Y. , Li W. J. , Stackebrandt E. . ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59:, 589–608. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052092-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052092-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error