1887

Abstract

A novel actinomycete strain, designed MW4-36, was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus . The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and -diaminopimelic acid including 3-hydroxy--diaminopimelic acid; arabinose, glucose, rhamnose, ribose and xylose were found as whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; no phosphatidylcholine was found. The predominant menaquinones were MK-9(H) and MK-9(H). Mycolic acids were not detected. The predominant cellular fatty acids were iso-C, iso-C, anteiso-C and anteiso-C. The G+C content of the genomic DNA was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that the strain should be classified in the genus and showed that the closest relative was JCM 15933 (99.4 % similarity). These taxonomic data revealed that strain MW4-36 could be readily distinguished from its phylogenetically closest relative. On the basis of these phenotypic and genotypic data, strain MW4-36 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is MW4-36 ( = BCC 34901 = NBRC 106093).

Funding
This study was supported by the:
  • National Research Council of Thailand (NRCT)
  • Actinobacterial Research Unit and Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang
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2013-11-01
2021-10-20
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