1887

Abstract

A facultatively anaerobic, Gram-staining-negative, pale red-pigmented, non-motile, rod-shaped, moderately halophilic bacterium, designated strain YIM J14, was isolated from a sediment sample from a salt mine in Yunnan, south-western China. Growth occurred at NaCl concentrations of between 2 % and 15 % (w/v) and optimally with 5–9 % NaCl. The optimum temperature and pH for growth of the strain were 28 °C and pH 7.5. The major cellular fatty acids were iso-C, anteiso-C and iso-Cω9/10-methyl-C. The polar lipid profile was composed predominantly of diphosphatidylglycerol, phosphatidylcholine and one unknown phospholipid. Minor amounts of other lipids were also detectable. The genomic DNA G+C content was 47.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain YIM J14 was related to in the phylum . The level of 16S rRNA gene sequence similarity between strain YIM J14 and CL-CB462 was 96.9 %. A DNA–DNA hybridization experiment between strain YIM J14 and indicated levels of relatedness of 28 %. Chemotaxonomic data supported the placement of strain YIM J14 in the genus . DNA–DNA hybridization and biochemical and physiological characterization allowed strain YIM J14 to be differentiated from . It is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain YIM J14 ( = ACCC 10717 = DSM 21985).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.052043-0
2013-11-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/3989.html?itemId=/content/journal/ijsem/10.1099/ijs.0.052043-0&mimeType=html&fmt=ahah

References

  1. Allgaier M. , Uphoff H. , Felske A. , Wagner-Döbler I. . ( 2003; ). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. . Appl Environ Microbiol 69:, 5051–5059. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cho J. C. , Giovannoni S. J. . ( 2006; ). Pelagibaca bermudensis gen. nov., sp. nov., a novel marine bacterium within the Roseobacter clade in the order Rhodobacterales . . Int J Syst Evol Microbiol 56:, 855–859. [CrossRef] [PubMed]
    [Google Scholar]
  4. Choi D. H. , Zhang G. I. , Noh J. H. , Kim W. S. , Cho B. C. . ( 2009; ). Gracilimonas tropica gen. nov., sp. nov., isolated from a Synechococcus culture. . Int J Syst Evol Microbiol 59:, 1167–1172. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cohen-Bazire G. , Sistrom W. R. , Stanier R. Y. . ( 1957; ). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. . J Cell Physiol 49:, 25–68. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  7. Cui X. L. , Mao P. H. , Zeng M. , Li W. J. , Zhang L. P. , Xu L. H. , Jiang C. L. . ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  8. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  9. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. Felsenstein, J. (1993). phylip (phylogeny inference package), version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  13. Garrity G. M. , Holt J. G. . ( 2001; ). The road map to the manual. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 119–166. Edited by Boone D. R. , Castenholz R. W. , Garrity G. M. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  14. Gregersen T. . ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 123–127. [CrossRef]
    [Google Scholar]
  15. Hansen G. H. , Sørheim R. . ( 1991; ). Improved method for phenotypical characterization of marine bacteria. . J Microbiol Methods 13:, 231–241. [CrossRef]
    [Google Scholar]
  16. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kovacs N. . ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703–704. [CrossRef] [PubMed]
    [Google Scholar]
  18. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95.[CrossRef]
    [Google Scholar]
  20. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  21. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  22. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Microbiol, pp. 611–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  24. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  25. Wang Y.‐X. , Liu Ji. ‐H. , Xiao W. , Zhang X.‐X. , Li Y.‐Q. , Lai Y.‐H. , Ji K.‐Y. , Wen M.‐L. , Cui X.‐L. . ( 2012; ). Fodinibius salinus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine . . Int J Syst Evol Microbiol 62:, 390–396.[CrossRef]
    [Google Scholar]
  26. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.052043-0
Loading
/content/journal/ijsem/10.1099/ijs.0.052043-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error