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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_11

sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-western China Free

Abstract

A facultatively anaerobic, Gram-staining-negative, pale red-pigmented, non-motile, rod-shaped, moderately halophilic bacterium, designated strain YIM J14, was isolated from a sediment sample from a salt mine in Yunnan, south-western China. Growth occurred at NaCl concentrations of between 2 % and 15 % (w/v) and optimally with 5–9 % NaCl. The optimum temperature and pH for growth of the strain were 28 °C and pH 7.5. The major cellular fatty acids were iso-C, anteiso-C and iso-Cω9/10-methyl-C. The polar lipid profile was composed predominantly of diphosphatidylglycerol, phosphatidylcholine and one unknown phospholipid. Minor amounts of other lipids were also detectable. The genomic DNA G+C content was 47.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain YIM J14 was related to in the phylum . The level of 16S rRNA gene sequence similarity between strain YIM J14 and CL-CB462 was 96.9 %. A DNA–DNA hybridization experiment between strain YIM J14 and indicated levels of relatedness of 28 %. Chemotaxonomic data supported the placement of strain YIM J14 in the genus . DNA–DNA hybridization and biochemical and physiological characterization allowed strain YIM J14 to be differentiated from . It is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain YIM J14 ( = ACCC 10717 = DSM 21985).

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Funding
This study was supported by the:
  • Ministry of Environmental Protection of China (Award 2012ZX07102-003)
  • National Natural Science Foundation of China (NSFC) (Award 21102126, 31200138, 31160123, 31000003 and 30860013)
  • Ministry of Science and Technology of China (Award 2007AA021306)
  • Yunnan Provincial Sciences and Technology Department (Award 2009DA002, 2009CD012 and 2005PY01-1)
  • Yunnan Provincial Education Department (Award 2010Y234 and 09Z0006)
  • Yunnan University (Award 2009C14Q, 2010YB002 and 2008YB005)
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2013-11-01
2025-04-25
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Gracilimonasmengyeensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-western China
Int J Syst Evol Microbiol 63, 3989 (2013); https://doi.org/10.1099/ijs.0.052043-0
/content/journal/ijsem/10.1099/ijs.0.052043-0
/content/journal/ijsem/10.1099/ijs.0.052043-0
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