1887

Abstract

A novel Gram-negative, yellow-pigmented, aerobic, motile by gliding, rod-shaped marine bacterium (JLT2011) was isolated from surface seawater. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JLT2011 could be assigned to the genus and was most closely related to , with 96.2 % similarity. The genomic DNA G+C content was 42.1 %. The predominant fatty acids were C, iso-C, C and summed feature 3 (Cω6/Cω7). The major menaquinone was MK-6. The major components of the polar lipid profile were phosphatidylglycerol, diphosphatidylglycerol and sphingoglycolipid. On the basis of phenotypic, genotypic and taxonomic data presented, strain JLT2011 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is JLT2011 ( = CGMCC 1.12375 = LMG 27360).

Funding
This study was supported by the:
  • National Key Basic Research Program of China (Award 2011CB808800)
  • 863 Program (Award 2012AA092003)
  • National Natural Science Foundation of China (Award 41276131 and 41191021)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051987-0
2014-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/1/165.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051987-0&mimeType=html&fmt=ahah

References

  1. Cho S. H., Chae S. H., Cho M., Kim T. U., Choi S., Han J. H., Kim Y. T., Joung Y., Joh K. & other authors ( 2011 ). Gramella gaetbulicola sp. nov., a member of the family Flavobacteriaceae isolated from foreshore soil. . Int J Syst Evol Microbiol 61, 26542658. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D., Goodfellow M., Minnikin D. E. ( 1980 ). Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. . J Gen Microbiol 118, 2937.[PubMed]
    [Google Scholar]
  3. Fraser S. L., Jorgensen J. H. ( 1997 ). Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. . Antimicrob Agents Chemother 41, 27382741.[PubMed]
    [Google Scholar]
  4. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  5. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  6. Kates M. ( 1986 ). Techniques of Lipidology: Isolation, Analysis and Identification of Lipids (Laboratory Techniques in Biochemistry and Molecular Biology), vol 3, part 2, , 2nd edn., Amsterdam:: Elsevier;
    [Google Scholar]
  7. Kim S. B., Falconer C., Williams E., Goodfellow M. ( 1998 ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. . Int J Syst Bacteriol 48, 5968. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. ( 2004 ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5, 150163. [View Article] [PubMed]
    [Google Scholar]
  11. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Dobretsov S., Wong P. K., Qian P. Y. ( 2005 ). Gramella portivictoriae sp. nov., a novel member of the family Flavobacteriaceae isolated from marine sediment. . Int J Syst Evol Microbiol 55, 24972500. [View Article] [PubMed]
    [Google Scholar]
  12. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  13. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S., Lee D. H., Kim I. S. ( 2005 ). Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius . . Int J Syst Evol Microbiol 55, 391394. [View Article] [PubMed]
    [Google Scholar]
  14. Nedashkovskaya O. I., Kim S. B., Bae K. S. ( 2010 ). Gramella marina sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . . Int J Syst Evol Microbiol 60, 27992802. [View Article] [PubMed]
    [Google Scholar]
  15. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  16. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [View Article]
    [Google Scholar]
  17. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051987-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051987-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error