1887

Abstract

Three independent strains of a rapidly growing, non-chromogenic member of the genus were isolated from lymph nodes of French cattle. Identification of the isolates was carried out using a polyphasic approach. The nearly complete SSU rRNA gene sequences (>1200 bp) of the strains MLB-A23, MLB-A30 and MLB-A84 were identical. A phylogenetic analysis of these unique SSU rRNA gene sequences showed that these strains were most closely related to . Further phylogenetic analysis based on concatenated sequences (2854 bp) of four housekeeping genes (, , and ), the transfer–messenger RNA (tmRNA) and SSU rRNA genes indicated that these three strains represented a distinct species that shares a common ancestor with . Phylogenetic and phenotypic data strongly indicate that the strains MLB-A23, MLB-A30 and MLB-A84 belong to a novel mycobacterial species for which the name sp. nov. is proposed. The type strain is MLB-A84 ( = CIP 110557 = DSM 45746).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051979-0
2013-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4669.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051979-0&mimeType=html&fmt=ahah

References

  1. Adékambi T., Drancourt M. ( 2004 ). Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. . Int J Syst Evol Microbiol 54, 20952105. [View Article] [PubMed]
    [Google Scholar]
  2. Adékambi T., Stein A., Carvajal J., Raoult D., Drancourt M. ( 2006 ). Description of Mycobacterium conceptionense sp. nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation. . J Clin Microbiol 44, 12681273. [View Article] [PubMed]
    [Google Scholar]
  3. Bos D. H., Posada D. ( 2005 ). Using models of nucleotide evolution to build phylogenetic trees. . Dev Comp Immunol 29, 211227. [View Article] [PubMed]
    [Google Scholar]
  4. Camacho C., Coulouris G., Avagyan V., Ma N., Papadopoulos J., Bealer K., Madden T. L. ( 2009 ). blast+: architecture and applications. . BMC Bioinformatics 10, 421. [View Article] [PubMed]
    [Google Scholar]
  5. Case R. J., Boucher Y., Dahllöf I., Holmström C., Doolittle W. F., Kjelleberg S. ( 2007 ). Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. . Appl Environ Microbiol 73, 278288. [View Article] [PubMed]
    [Google Scholar]
  6. Devulder G., Pérouse de Montclos M., Flandrois J.-P. ( 2005 ). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. . Int J Syst Evol Microbiol 55, 293302. [View Article] [PubMed]
    [Google Scholar]
  7. Edgar R. C. ( 2004 ). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32, 17921797. [View Article] [PubMed]
    [Google Scholar]
  8. Edson R. S., Terrell C. L., Brutinel W. M., Wengenack N. L. ( 2006 ). Mycobacterium intermedium granulomatous dermatitis from hot tub exposure. . Emerg Infect Dis 12, 821823. [View Article] [PubMed]
    [Google Scholar]
  9. Felske A., Engelen B., Nübel U., Backhaus H. ( 1996 ). Direct ribosome isolation from soil to extract bacterial rRNA for community analysis. . Appl Environ Microbiol 62, 41624167.[PubMed]
    [Google Scholar]
  10. Gascuel O. ( 1997 ). bionj: an improved version of the NJ algorithm based on a simple model of sequence data. . Mol Biol Evol 14, 685695. [View Article] [PubMed]
    [Google Scholar]
  11. Gouy M., Guindon S., Gascuel O. ( 2010 ). SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. . Mol Biol Evol 27, 221224. [View Article] [PubMed]
    [Google Scholar]
  12. Guindon S., Gascuel O. ( 2003 ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52, 696704. [View Article] [PubMed]
    [Google Scholar]
  13. Guindon S., Dufayard J.-F., Lefort V., Anisimova M., Hordijk W., Gascuel O. ( 2010 ). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. . Syst Biol 59, 307321. [View Article] [PubMed]
    [Google Scholar]
  14. Ito A., Kishi F., Saito N., Kazumi Y., Mitarai S. ( 2005 ). Pulmonary Mycobacterium intermedium disease in an elderly man with healed pulmonary tuberculosis. . J Clin Microbiol 43, 14731474. [View Article] [PubMed]
    [Google Scholar]
  15. Kik M. J. L., Houwers D. J., Dinkla A. ( 2010 ). Mycobacterium intermedium granulomatous pneumonia in a green oropendola (Psarocolius viridis). . Vet Rec 167, 219220. [View Article] [PubMed]
    [Google Scholar]
  16. Kirschner P., Teske A., Schröder K.-H., Kroppenstedt R. M., Wolters J., Böttger E. C. ( 1992 ). Mycobacterium confluentis sp. nov.. Int J Syst Bacteriol 42, 257262. [View Article] [PubMed]
    [Google Scholar]
  17. Magee J. G., Ward A. C. ( 2012 ). Genus I. Mycobacterium Lehmann and Neuman 1896, 363AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 5, pp. 312375. Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  18. Meier A., Kirschner P., Schröder K.-H., Wolters J., Kroppenstedt R. M., Böttger E. C. ( 1993 ). Mycobacterium intermedium sp. nov.. Int J Syst Bacteriol 43, 204209. [View Article] [PubMed]
    [Google Scholar]
  19. Mignard S., Flandrois J.-P. ( 2007 ). Identification of Mycobacterium using the EF-Tu encoding (tuf) gene and the tmRNA encoding (ssrA) gene. . J Med Microbiol 56, 10331041. [View Article] [PubMed]
    [Google Scholar]
  20. Mignard S., Flandrois J.-P. ( 2008 ). A seven-gene, multilocus, genus-wide approach to the phylogeny of mycobacteria using supertrees. . Int J Syst Evol Microbiol 58, 14321441. [View Article] [PubMed]
    [Google Scholar]
  21. Schinsky M. F., Morey R. E., Steigerwalt A. G., Douglas M. P., Wilson R. W., Floyd M. M., Butler W. R., Daneshvar M. I., Brown-Elliott B. A. & other authors ( 2004 ). Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates. . Int J Syst Evol Microbiol 54, 16531667. [View Article] [PubMed]
    [Google Scholar]
  22. Staden R., Beal K. F., Bonfield J. K. ( 2000 ). The Staden package, 1998. . Methods Mol Biol 132, 115130.[PubMed]
    [Google Scholar]
  23. Tortoli E. ( 2003 ). Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. . Clin Microbiol Rev 16, 319354. [View Article] [PubMed]
    [Google Scholar]
  24. Tortoli E. ( 2006 ). The new mycobacteria: an update. . FEMS Immunol Med Microbiol 48, 159178. [View Article] [PubMed]
    [Google Scholar]
  25. Vincent V., Brown-Elliott B. A., Jost K. C. Jr, Wallace R. J. ( 2003 ). Mycobacterium phenotypic and genotypic identification. . In Manual of Clinical Microbiology, , 8th edn., pp. 560584. Edited by Murray P. M., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. J. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051979-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051979-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error