1887

Abstract

Two bacterial strains, designated IWT246 and IWT248, were isolated from orchardgrass ( L.) silage from Iwate prefecture, Japan, and examined for a taxonomic study. Both organisms were rod-shaped, Gram-stain-positive, catalase-negative, facultatively anaerobic and homofermentative. The cell wall did not contain -diaminopimelic acid and the major fatty acids were Cω9 and C cyclo 9,10/:1. Comparative analyses of 16S rRNA, and gene sequences revealed that these strains were novel and belonged to the genus . Based on 16S rRNA gene sequence similarity, the isolates were most closely related to the type strains of the following members of the genus : (96.7 % similarity), (96.6 %), (95.5 %) and (94.1 %). However, the 16S rRNA gene sequences of both IWT246 and IWT248 were 99.7 % similar to that of ‘ ’ JCM 18665; this name has not been validly published. Genotypic, phenotypic and chemotaxonomic analyses confirmed that these novel strains occupy a unique taxonomic position. DNA–DNA hybridization experiments demonstrated genotypic separation of the novel isolates from related species. The name sp. nov. is proposed for the novel isolates, with strain IWT246 ( = JCM 18838 = DSM 26942) as the type strain. Our results also suggest that ‘’ does represent a novel species. The cells did not contain -diaminopimelic acid in their cell-wall peptidoglycan and the major fatty acids were C, C cyclo 9,10/:1 and summed feature 10 (one or more of Cω11, Cω9, Cω6 and unknown ECL 17.834). We therefore propose the corrected name sp. nov., with the type strain JCM 18665 ( = LMG 23555 = DSM 18080 = L1062).

Funding
This study was supported by the:
  • , Grant-in-Aid for Young Scientists (A) , (Award 25712032)
  • , JST/JICA, SATREPS
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2013-10-01
2020-12-04
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