1887

Abstract

A Gram-stain-negative, non-spore-forming, rod-shaped, aerobic bacterium (strain 107-E2) was isolated from freshwater samples containing microbial mats collected at a lake in Skarvsnes, Antarctica (temporary lake name, Lake Tanago Ike). Strain 107-E2 grew between 5 and 25 °C, with an optimum of 23 °C. Moreover, colony formation was observed on agar media even at −5 °C. The pH range for growth was between 6.0 and 9.0, with an optimum of pH 7.0–8.0. The range of NaCl concentration for growth was between 0.0 and 0.5 % (w/v), with an optimum of 0.0 %. No growth was observed in media containing organic compounds at high concentrations, which indicated that strain 107-E2 was an oligotroph. In the late stationary phase, strain 107-E2 produced a dark brown water-soluble pigment. Esterase, amylase and protease production was observed. Antimicrobial-lytic activities for Gram-negative bacteria and yeast were observed. Ubiquinone-8 was the major respiratory quinone. The major fatty acids were iso-C, iso-Cω9 and iso-C at 5. The G+C content of genomic DNA was 66.1 mol%. Analysis of the 16S rRNA gene sequences revealed that strain 107-E2 belonged to the genus , and low DNA–DNA relatedness values with closely related species distinguished strain 107-E2 from recognized species of the genus . The phylogenetic situation and physiological characteristics indicated that strain 107-E2 should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 107-E2 ( = JCM 18257 = ATCC BAA-2438).

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2013-09-01
2019-10-19
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Bae H. S. , Im W. T. , Lee S. T. . ( 2005; ). Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. . Int J Syst Evol Microbiol 55:, 1155–1161. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bargagli R. . ( 2005; ). Antarctica: geomorphology and climate trends. . In Antarctic Ecosystems: Environmental Contamination, Climate Change, and Human Impact, pp. 1–40. Berlin:: Springer;.
    [Google Scholar]
  4. Cabanes J. , Chazarra S. , Garcia-Carmona F. . ( 1994; ). Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. . J Pharm Pharmacol 46:, 982–985. [CrossRef] [PubMed]
    [Google Scholar]
  5. Christensen P. . ( 2005; ). Genus IV. Lysobacter Christensen and Cook 1978, 372AL . . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol 2, part B, pp. 95–101. Edited by Brenner D. J. , Kreig N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  6. Christensen P. , Cook F. D. . ( 1978; ). Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. . Int J Syst Bacteriol 28:, 367–393. [CrossRef]
    [Google Scholar]
  7. Claus H. , Decker H. . ( 2006; ). Bacterial tyrosinases. . Syst Appl Microbiol 29:, 3–14. [CrossRef] [PubMed]
    [Google Scholar]
  8. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  9. Fukuda W. , Yamada K. , Miyoshi Y. , Okuno H. , Atomi H. , Imanaka T. . ( 2012; ). Rhodoligotrophos appendicifer gen. nov., sp. nov., an appendaged bacterium isolated from a freshwater Antarctic lake. . Int J Syst Evol Microbiol 62:, 1945–1950. [CrossRef] [PubMed]
    [Google Scholar]
  10. Imura S. , Bando T. , Saito S. , Seto K. , Kanda H. . ( 1999; ). Benthic moss pillars in Antarctic lakes. . Polar Biol 22:, 137–140. [CrossRef]
    [Google Scholar]
  11. Imura S. , Bando T. , Seto K. , Ohtani S. , Kudoh S. , Kanda H. . ( 2003; ). Distribution of aquatic mosses in the Soya Coast region, East Antarctica. . Polar Biosci 16:, 1–10.
    [Google Scholar]
  12. Jung H. M. , Ten L. N. , Im W. T. , Yoo S. A. , Lee S. T. . ( 2008; ). Lysobacter ginsengisoli sp. nov., a novel species isolated from soil in Pocheon Province, South Korea. . J Microbiol Biotechnol 18:, 1496–1499.[PubMed]
    [Google Scholar]
  13. Kawamura Y. , Tomida J. , Morita Y. , Naka T. , Mizuno S. , Fujiwara N. . ( 2009; ). Lysobacter enzymogenes ssp. cookii ’ Christensen 1978 should be recognized as an independent species, Lysobacter cookii sp. nov.. FEMS Microbiol Lett 298:, 118–123. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lee J. W. , Im W. T. , Kim M. K. , Yang D. C. . ( 2006; ). Lysobacter koreensis sp. nov., isolated from a ginseng field. . Int J Syst Evol Microbiol 56:, 231–235. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ohtsuka T. , Kudoh S. , Imura S. , Ohtani S. . ( 2006; ). Diatoms composing benthic microbial mats in freshwater lakes of Skarvsnes ice-free area, East Antarctica. . Polar Biosci 20:, 113–130.
    [Google Scholar]
  16. Park J. H. , Kim R. , Aslam Z. , Jeon C. O. , Chung Y. R. . ( 2008; ). Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter . . Int J Syst Evol Microbiol 58:, 387–392. [CrossRef] [PubMed]
    [Google Scholar]
  17. Reasoner D. J. , Geldreich E. E. . ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49:, 1–7.[PubMed]
    [Google Scholar]
  18. Romanenko L. A. , Uchino M. , Tanaka N. , Frolova G. M. , Mikhailov V. V. . ( 2008; ). Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. . Int J Syst Evol Microbiol 58:, 370–374. [CrossRef] [PubMed]
    [Google Scholar]
  19. Saddler G. S. , Bradbury J. F. . ( 2005; ). Order III. Xanthomonadales ord. nov.. In In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol 2, part B, pp. 63. Edited by Brenner D. J. , Kreig N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  20. Sambrook J. , Russell D. W. . ( 2001; ). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  21. Srinivasan S. , Kim M. K. , Sathiyaraj G. , Kim H. B. , Kim Y. J. , Yang D. C. . ( 2010; ). Lysobacter soli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 60:, 1543–1547. [CrossRef] [PubMed]
    [Google Scholar]
  22. Ten L. N. , Jung H. M. , Im W. T. , Yoo S. A. , Oh H. M. , Lee S. T. . ( 2009; ). Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 59:, 958–963. [CrossRef] [PubMed]
    [Google Scholar]
  23. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wang Y. , Dai J. , Zhang L. , Luo X. , Li Y. , Chen G. , Tang Y. , Meng Y. , Fang C. . ( 2009; ). Lysobacter ximonensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59:, 786–789. [CrossRef] [PubMed]
    [Google Scholar]
  25. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky L. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  26. Weon H. Y. , Kim B. Y. , Baek Y. K. , Yoo S. H. , Kwon S. W. , Stackebrandt E. , Go S. J. . ( 2006; ). Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. . Int J Syst Evol Microbiol 56:, 947–951. [CrossRef] [PubMed]
    [Google Scholar]
  27. Weon H. Y. , Kim B. Y. , Kim M. K. , Yoo S. H. , Kwon S. W. , Go S. J. , Stackebrandt E. . ( 2007; ). Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 57:, 548–551. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yamada K. , Fukuda W. , Kondo Y. , Miyoshi Y. , Atomi H. , Imanaka T. . ( 2011; ). Constrictibacter antarcticus gen. nov., sp. nov., a cryptoendolithic micro-organism from Antarctic white rock. . Int J Syst Evol Microbiol 61:, 1973–1980. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yoshimoto T. , Yamamoto K. , Tsuru D. . ( 1985; ). Extracellular tyrosinase from Streptomyces sp. KY-453: purification and some enzymatic properties. . J Biochem 97:, 1747–1754.[PubMed]
    [Google Scholar]
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