1887

Abstract

A Gram-stain-positive actinobacterium, designated strain SCSIO 15020, was isolated from sediment of the South China Sea, and characterized by using a polyphasic approach. The temperature range for growth was 24–60 °C, with optimal growth occurring at 50 °C. The pH range for growth was 6–10 (optimum pH 8–9). The NaCl concentration range for growth was 0–5 % (w/v). The peptidoglycan type was A4α. Polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown polar lipid. The major menaquinone was MK-8(H); MK-7(H) was present as a minor component. The major fatty acids (>5 %) were anteiso-C iso-C and iso-C. The DNA G+C content of strain SCSIO 15020 was 73.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 15020 belonged to the genus , with the closest neighbours being 1A-C (96.3 % similarity), TT02-04 (95.7 %) and YIM 004 (95.6 %). Based on evidence from the present polyphasic study, strain SCSIO 15020 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 15020 ( = DSM 25884 = NBRC 108941).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051714-0
2013-11-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4243.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051714-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Schumann P., Vybiral D., Lubitz W., Busse H. J.. ( 2002;). Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. . Int J Syst Evol Microbiol 52:, 875–881. [CrossRef][PubMed]
    [Google Scholar]
  2. Collins M., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Microbiol 48:, 459–470. [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Fu Y., Li Q., Liu K., Xu Y., Wang Y., Jiao N.. ( 2012;). Oceanitalea nanhaiensis gen. nov., sp. nov., an actinobacterium isolated from seawater. . Int J Syst Evol Microbiol 62:, 2490–2494. [CrossRef][PubMed]
    [Google Scholar]
  7. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef][PubMed]
    [Google Scholar]
  8. Hamada M., Tamura T., Ishida Y., Suzuki K.. ( 2009;). Georgenia thermotolerans sp. nov., an actinobacterium isolated from forest soil. . Int J Syst Evol Microbiol 59:, 1875–1879. [CrossRef][PubMed]
    [Google Scholar]
  9. Kämpfer P., Arun A. B., Busse H. J., Langer S., Young C. C., Chen W. M., Schumann P., Syed A. A., Rekha P. D.. ( 2010;). Georgenia soli sp. nov., isolated from iron-ore-contaminated soil in India. . Int J Syst Evol Microbiol 60:, 1027–1030. [CrossRef][PubMed]
    [Google Scholar]
  10. Kelly K. L.. ( 1964;). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Leifson E.. ( 1960;). Atlas of Bacterial Flagellation. London:: Academic Press;.
    [Google Scholar]
  13. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  15. Minnikin D., Collins M., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Microbiol 47:, 87–95. [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  17. Schumann P.. ( 2011;). Peptidoglycan structure. . Methods Microbiol 38, 101–129. [CrossRef]
    [Google Scholar]
  18. Srinivas A., Rahul K., Sasikala Ch., Subhash Y., Ramaprasad E. V., Ramana ChV.. ( 2012;). Georgenia satyanarayanai sp. nov., an alkaliphilic and thermotolerant amylase-producing actinobacterium isolated from a soda lake. . Int J Syst Evol Microbiol 62:, 2405–2409. [CrossRef][PubMed]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  20. Tang S. K., Wang Y., Lee J. C., Lou K., Park D. J., Kim C. J., Li W. J.. ( 2010;). Georgenia halophila sp. nov., a halophilic actinobacterium isolated from a salt lake. . Int J Syst Evol Microbiol 60:, 1317–1321. [CrossRef][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  22. Woo S. G., Cui Y., Kang M. S., Jin L., Kim K. K., Lee S. T., Lee M., Park J.. ( 2012;). Georgenia daeguensis sp. nov., isolated from 4-chlorophenol enrichment culture. . Int J Syst Evol Microbiol 62:, 1703–1709. [CrossRef][PubMed]
    [Google Scholar]
  23. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051714-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051714-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error