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Volume 63, Issue Pt_10

sp. nov., a producer of telomycin, isolated from soil Free

Abstract

A Gram-stain-positive, spore-forming actinomycete strain (HKI0641) was isolated from a soil sample collected in the Black Forest, Germany. During screening for antimicrobial natural products this bacterium was identified as a producer of the antibiotic telomycin. Morphological characteristics and chemotaxonomic data indicated that the strain belonged to the genus . The peptidoglycan of strain HKI0641 contained -diaminopimelic acid, and the fatty acid profile consisted predominantly of anteiso-C, iso-C, iso-C and C. MK-10(H), MK-10(H) and MK-10 were identified as the major menaquinones. To determine the taxonomic positioning of strain HKI0641, we computed a binary tanglegram of two rooted phylogenetic trees that were based upon 16S rRNA and gene sequences. The comparative analysis of the two common classification methods strongly supported the phylogenetic affiliation with the genus , but it also revealed discrepancies in the assignment at the level of the genomic species. 16S rRNA gene sequence analysis identified DSM 45161 (99.1 % sequence similarity) and DSM 45555 (99.0 %) as the nearest taxonomic neighbours, whereas the sequence of strain HKI0641 indicated a closer relationship to DSM 43813 (95.1 %). By means of DNA–DNA hybridization experiments, it was possible to resolve this issue and to clearly differentiate strain HKI0641 from other species of the genus . The type strains of the aforementioned species of the genus could be further distinguished from strain HKI0641 by several phenotypic properties, such as colony colour, NaCl tolerance and the utilization of carbon sources. The isolate was therefore assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HKI0641 ( = DSM 45708 = CIP 110415).

  • Published Online:
Funding
This study was supported by the:
  • Deutsche Forschungsgemeinschaft (DFG)
  • Jena School for Microbial Communication (JSMC)
  • German Academic Exchange Service (DAAD) (Award A1071666)
© 2013 IUMS
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2013-10-01
2024-04-24
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Micromonosporaschwarzwaldensis sp. nov., a producer of telomycin, isolated from soil
Int J Syst Evol Microbiol 63, 3812 (2013); https://doi.org/10.1099/ijs.0.051623-0
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