1887

Abstract

A Gram-stain-positive, strictly aerobic, rod-shaped and weakly motile bacterium, designated WS 4627, was isolated from a dairy product sample collected in southern Germany. Spherical to slightly ellipsoidal endospores were formed centrally or subterminally in sometimes slightly swollen sporangia. The isolate was able to grow at 8–35 °C, at pH 6.5–8.5 and with 0.5–16.5 % (w/v) NaCl. The diamino acid of the cell wall was -diaminopimelic acid (peptidoglycan type A1γ) and the genomic DNA G+C content was 39.1 mol%. The major menaquinone was MK-7, the cellular fatty acid profile contained major amounts of anteiso-C and anteiso-C and the major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Strain WS 4627 was most closely related to ‘ ’ FarD (96.5 % 16S rRNA gene sequence similarity) and ‘ ’ JSM 079157 (96.0 %). Based on the data presented, strain WS 4627 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WS 4627 ( = DSM 25060 = LMG 26644).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051565-0
2013-09-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3358.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051565-0&mimeType=html&fmt=ahah

References

  1. Amziane M., Metiaz F., Darenfed-Bouanane A., Djenane Z., Selama O., Abderrahmani A., Cayol J.-L., Fardeau M.-L.. ( 2013;). Virgibacillus natechei sp. nov., a moderately halophilic bacterium isolated from sediment of a saline lake in southwest of Algeria. . Curr Microbiol 66:, 462–466. [CrossRef][PubMed]
    [Google Scholar]
  2. Cerny G.. ( 1976;). Method for distinction of the Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol 3:, 223–225. [CrossRef]
    [Google Scholar]
  3. Chen Y.-G., Cui X.-L., Fritze D., Chai L.-H., Schumann P., Wen M.-L., Wang Y.-X., Xu L.-H., Jiang C.-L.. ( 2008;). Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. . Int J Syst Evol Microbiol 58:, 647–653. [CrossRef][PubMed]
    [Google Scholar]
  4. Claus D., Berkeley R. C. W.. ( 1986;). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1140. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  5. Heyndrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A.. ( 1998;). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. . Int J Syst Bacteriol 48:, 99–106. [CrossRef]
    [Google Scholar]
  6. Heyndrickx M., Lebbe L., Kersters K., Hoste B., De Wachter R., De Vos P., Forsyth G., Logan N. A.. ( 1999;). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. . Int J Syst Bacteriol 49:, 1083–1090. [CrossRef][PubMed]
    [Google Scholar]
  7. Heyrman J., Logan N. A., Busse H.-J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P.. ( 2003;). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. . Int J Syst Evol Microbiol 53:, 501–511. [CrossRef][PubMed]
    [Google Scholar]
  8. Heyrman J., De Vos P., Logan N.. ( 2009;). Genus XIX. Virgibacillus. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 193–228. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  9. Kim Y.-G., Choi D. H., Hyun S., Cho B. C.. ( 2007;). Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. . Int J Syst Evol Microbiol 57:, 409–413. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim J., Jung M.-J., Roh S. W., Nam Y.-D., Shin K.-S., Bae J.-W.. ( 2011;). Virgibacillus alimentarius sp. nov., isolated from a traditional Korean food. . Int J Syst Evol Microbiol 61:, 2851–2855. [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Lee J.-S., Lim J.-M., Lee K. C., Lee J.-C., Park Y.-H., Kim C.-J.. ( 2006;). Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. . Int J Syst Evol Microbiol 56:, 251–257. [CrossRef][PubMed]
    [Google Scholar]
  13. Lee S.-Y., Kang C.-H., Oh T.-K., Yoon J.-H.. ( 2012;). Virgibacillus campisalis sp. nov., from a marine solar saltern. . Int J Syst Evol Microbiol 62:, 347–351. [CrossRef][PubMed]
    [Google Scholar]
  14. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L.. & other authors ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef][PubMed]
    [Google Scholar]
  15. Munoz R., Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K.-H., Glöckner F.-O., Rosselló-Móra R.. ( 2011;). Release LTPs104 of the All-Species Living Tree. . Syst Appl Microbiol 34:, 169–170. [CrossRef][PubMed]
    [Google Scholar]
  16. Niederberger T. D., Steven B., Charvet S., Barbier B., Whyte L. G.. ( 2009;). Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic. . Int J Syst Evol Microbiol 59:, 2219–2225. [CrossRef][PubMed]
    [Google Scholar]
  17. Peng Q.-Z., Chen J., Zhang Y.-Q., Chen Q.-H., Peng D.-J., Cui X.-L., Li W.-J., Chen Y.-G.. ( 2009;). Virgibacillus zhanjiangensis sp. nov., a marine bacterium isolated from sea water. . Antonie van Leeuwenhoek 96:, 645–652. [CrossRef][PubMed]
    [Google Scholar]
  18. Ryu E.. ( 1940;). A simple method of differentiation between Gram-positive and Gram-negative organisms without staining. . Kitasato Arch Exp Med 17:, 58–63.
    [Google Scholar]
  19. Schmidt V. S., Mayr R., Wenning M., Glöckner J., Busse H.-J., Scherer S.. ( 2009;). Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese. . Int J Syst Evol Microbiol 59:, 2437–2443. [CrossRef][PubMed]
    [Google Scholar]
  20. Seeley H. W., Vandemark P. J.. ( 1962;). Microbes in Action. A Laboratory Manual of Microbiology. San Francisco:: Freeman;.
    [Google Scholar]
  21. Seiler H., Schmidt V., Wenning M., Scherer S.. ( 2012a;). Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. . Int J Syst Evol Microbiol 62:, 1092–1097. [CrossRef][PubMed]
    [Google Scholar]
  22. Seiler H., Bleicher A., Busse H.-J., Hüfner J., Scherer S.. ( 2012b;). Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese. . Int J Syst Evol Microbiol 62:, 1850–1856. [CrossRef][PubMed]
    [Google Scholar]
  23. Seiler H., Wenning M., Scherer S.. ( 2013;). Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room. . Int J Syst Evol Microbiol 63:, 2054–2061. [CrossRef][PubMed]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  26. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P.. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef][PubMed]
    [Google Scholar]
  27. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J.-H., Oh T.-K., Park Y.-H.. ( 2004;). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov.. Int J Syst Evol Microbiol 54:, 2163–2167. [CrossRef][PubMed]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Jung Y.-T., Lee K. C., Oh H. W., Oh T.-K.. ( 2010;). Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern. . Int J Syst Evol Microbiol 60:, 291–295. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051565-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051565-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error