%0 Journal Article %A Losey, Nathaniel A. %A Stevenson, Bradley S. %A Busse, Hans-Jürgen %A Damsté, Jaap S. Sinninghe %A Rijpstra, W. Irene C. %A Rudd, Stephen %A Lawson, Paul A. %T Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_11 %P 4149-4157 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.051425-0 %I Microbiology Society, %X A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01T stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01T was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym-homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01T represents the first reported cultivated member of subdivision 23 of the Acidobacteria . It is proposed that strain MP-01T represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01T ( = DSM 24856T = JCM 18256T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.051425-0