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Volume 63, Issue Pt_11

gen. nov., sp. nov., the first cultivated member of subdivision 23, isolated from a hot spring Free

Abstract

A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01 stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01 was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C, iso-C, anteiso-C and iso-C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01 represents the first reported cultivated member of subdivision 23 of the . It is proposed that strain MP-01 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is MP-01 ( = DSM 24856 = JCM 18256).

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Funding
This study was supported by the:
  • US National Park Service
  • US Department of the Interior
© 2013 IUMS
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2013-11-01
2024-04-16
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Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring
Int J Syst Evol Microbiol 63, 4149 (2013); https://doi.org/10.1099/ijs.0.051425-0
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