1887

Abstract

A novel bacterial strain, designated M8, was isolated from milk of a female macaque bred in captivity. The strain was Gram-stain-positive, anaerobic, irregular coccoid–rod-shaped without catalase activity. Analysis of 16S rRNA gene sequence similarity revealed that the isolate was most closely related to CCUG 31649 (96.4 %) and OMB105 (96.6 %). Sequences of , , and genes also confirmed that the strain was most closely related to the type strains of and . The isolate produced fructose-6-phosphate phosphoketolase which is in agreement with classification within the family . The major fatty acids were Cω9 (35.8 %), C (6.2 %) and C (5.7 %). Polar lipid analysis revealed five different glycolipids, two unidentified phospholipids and diphosphatidylglycerol. The peptidoglycan was of the type A4α -Lys–-Asp with the presence of ()-alanine, -glutamine, -asparagine and -lysine. The DNA G+C content of strain M8 was 50.1 mol%. On the basis of genetic, phylogenetic and phenotypic data, strain M8 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is M8 ( = DSM 24762 = CCM 7944). In addition, our results also revealed that DSM 21503 and DSM 17774 do not belong to different genera within the family . We therefore propose to reclassify as comb. nov. An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , Ministry of Agriculture of the Czech Republic , (Award NAZV QJ1210093)
  • , Grant Agency of the Czech Republic , (Award 523/08/1091)
  • , Institutional Research Project of the Institute of Animal Physiology and Genetics, Acad. Sci. CR , (Award 67985904)
  • , Institutional Research Concept RVO , (Award 61388971)
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2013-12-01
2021-02-28
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