Two yellow-pigmented, rod-shaped, non-motile, Gram-reaction-negative and aerobic bacterial strains, designated KYW560T and KYW563T, were isolated from seawater collected from Gwangyang Bay, Republic of Korea. The isolates required sea salts for growth. Flexirubin-type pigments were absent. The common major cellular fatty acids (>5 % of total) of the two strains were C16 : 0, C18 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Strain KYW560T also contained iso-C15 : 0 3-OH and C20 : 1ω9c as major fatty acids. The main polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The predominant isoprenoid quinone was MK-6. The DNA G+C contents of strains KYW560T and KYW563T were 41.0±0.7 and 38.3±0.4 mol% (mean±sd of three determinations), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates belonged to the family Flavobacteriaceae, and were related to the genus Algibacter. Based on data from this taxonomic study using a polyphasic approach, it is proposed that the isolates represent novel species of the genus Algibacter, for which the names Algibacteragarivorans sp. nov. (type strain, KYW560T = KCTC 23855T = JCM 18285T) and Algibacteragarilyticus sp. nov. (type strain, KYW563T = KCTC 23857T = JCM 18275T) are proposed. Reclassification of Marinivirga aestuarii as Algibacteraestuarii comb. nov. and emended description of the genus Algibacter are also proposed.
BernardetJ.-F.(2011). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by
WhitmanW.
. Baltimore:: Williams & Wilkins;.
BernardetJ.-F.,
NakagawaY.,
HolmesB.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol52, 1049–1070. [View Article][PubMed]
ChunJ.,
GoodfellowM.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol45, 240–245. [View Article][PubMed]
CollinsM. D.(1994). Isoprenoid quinones. . In Chemical Methods in Prokaryotic Systematics, pp. 265–309. Edited by
GoodfellowM.,
O’DonnellA. G.
. Chichester:: Wiley;.
FelsensteinJ.(1993).phylip (phylogeny inference package), version 3.5c.. Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24, 54–63. [View Article]
JukesT. H.,
CantorC. R.(1969). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by
MunroH. N.
. New York:: Academic Press;.
MarmurJ.,
DotyP.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5, 109–118. [View Article][PubMed]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
NedashkovskayaO. I.,
KimS. B.,
HanS. K.,
RheeM. S.,
LysenkoA. M.,
RohdeM.,
ZhukovaN. V.,
FrolovaG. M.,
MikhailovV. V.,
BaeK. S.(2004).Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. . Int J Syst Evol Microbiol54, 1257–1261. [View Article][PubMed]
NedashkovskayaO. I.,
VancanneytM.,
KimS. B.,
HosteB.,
BaeK. S.(2007).Algibacter mikhailovii sp. nov., a novel marine bacterium of the family Flavobacteriaceae, and emended description of the genus Algibacter
. . Int J Syst Evol Microbiol57, 2147–2150. [View Article][PubMed]
ParkS. C.,
ChoeH. N.,
HwangY. M.,
BaikK. S.,
KimS. N.,
LeeY. S.,
JungJ. S.,
SeongC. N.(2013a).Marinivirga aestuarii gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from marine environments, and emended descriptions of the genera Hyunsoonleella, Jejuia and Pontirhabdus and the species Hyunsoonleella jejuensis, Jejuia pallidilutea and Pontirhabdus pectinivorans
. . Int J Syst Evol Microbiol63, 1524–1531. [View Article][PubMed]
ParkS. C.,
HwangY. M.,
ChoeH. N.,
BaikK. S.,
KimH.,
SeongC. N.(2013b).Algibacter aquimarinus sp. nov., isolated from a marine environment, and reclassification of Pontirhabdus pectinivorans as Algibacter pectinivorans comb. nov.. Int J Syst Evol Microbiol63, 2038–2042. [View Article][PubMed]
SmibertR. M.,
KriegN. R.(1994). General characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GebhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
TindallB. J.,
SikorskiJ.,
SmibertR. A.,
KriegN. R.(2007). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by
ReddyC. A.,
BeveridgeT. J.,
BreznakJ. A.,
MarzlufG.,
SchmidtT. M.,
SnyderL. R.
. Washington, DC:: American Society for Microbiology;.
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
YumotoI.,
HirotaK.,
YamagaS.,
NodasakaY.,
KawasakiT.,
MatsuyamaH.,
NakajimaK.(2004).Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. . Int J Syst Evol Microbiol54, 1997–2001. [View Article][PubMed]
Algibacteragarivorans sp. nov. and Algibacteragarilyticus sp. nov., isolated from seawater, reclassification of Marinivirga aestuarii as Algibacteraestuarii comb. nov. and emended description of the genus Algibacter