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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_12

sp. nov., with ginsenoside-converting activity Free

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain, designated THG-45, was isolated from soil of a ginseng field of Pocheon province in the Republic of Korea and its taxonomic position was investigated by a polyphasic approach. Growth occurred at 4–30 °C, at pH 5.5–9.0 and with 0–2 % (w/v) NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity, strain THG-45 was shown to belong to the genus and was related to G-1 (98.8 %), NWER-II11 (97.9 %), PB92 (97.9 %), DS-57 (97.5 %), 15-52 (97.4 %), DS-27 (97.0 %) and 15-51 (97.0 %), but DNA relatedness between strain THG-45 and these strains was below 36 %. The G+C content of the genomic DNA was 39 mol%. The only isoprenoid quinone detected in strain THG-45 was menaquinone-7 (MK-7). The predominant fatty acids were iso-C, summed feature 3 (comprising Cω6 and/or Cω7) and iso-C 3-OH, and the major polar lipids were phosphatidylethanolamine and an unidentified aminophosphoglycolipid. Phenotypic data and phylogenetic inference supported the affiliation of strain THG-45 to the genus , and a number of biochemical tests differentiated strain THG-45 from the recognized species of the genus . Therefore, the novel isolate represents a novel species, for which the name sp. nov. is proposed, with THG-45 as the type strain ( = KACC 14530 = JCM 16721).

  • Published Online:
Funding
This study was supported by the:
  • Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Award 810006-03-1-CG100)
© 2013 IUMS
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2013-12-01
2024-04-25
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Pedobacter ginsenosidimutans sp. nov., with ginsenoside-converting activity
Int J Syst Evol Microbiol 63, 4396 (2013); https://doi.org/10.1099/ijs.0.051102-0
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