1887

Abstract

A bacterial consortium that accumulated more than 90 % (w/w) polyhydroxybutyrate (PHB) from lactate was selected in a laboratory-scale bioreactor with a ‘feast–famine’ regime. Bacterial strain YD, representing a dominant species in this enrichment, was isolated and characterized. Analysis of the 16S rRNA gene sequence revealed that the isolate is a member of the class , forming an independent phylogenetic lineage. The closest relative of the isolate was TUD-YJ37, with 94 % 16S rRNA gene sequence similarity. Strain YD was an obligate aerobe with large, ovoid, Gram-negative cells, motile by means of a polar flagellum. It utilized a relatively broad spectrum of substrates (e.g. carbohydrates, fatty acids) as carbon and energy sources. The temperature range for growth was 20–45 °C, with an optimum at 40 °C; the pH range was pH 6.0–8.0, with an optimum at pH 7.0. The major respiratory lipoquinones were Q-8 (91 %) and Q-7 (9 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and an unidentified aminolipid. The predominant fatty acids in the membrane polar lipids were Cω7, C and Cω7. The G+C content of the genomic DNA was 68.5 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolate is proposed to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is YD ( = DSM 25287 = NCCB 100398).

Funding
This study was supported by the:
  • , Dutch Technology Foundation (STW) , (Award 11604)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051045-0
2014-01-01
2020-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/1/33.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051045-0&mimeType=html&fmt=ahah

References

  1. Asselineau J., Trüper H. G. ( 1982 ). Lipid composition of six species of the phototrophic bacterial genus Ectothiorhodospira . . Biochim Biophys Acta 712, 111116. [CrossRef]
    [Google Scholar]
  2. Braunegg G., Lefebvre G., Genser K. F. ( 1998 ). Polyhydroxyalkanoates, biopolyesters from renewable resources: physiological and engineering aspects. . J Biotechnol 65, 127161. [CrossRef] [PubMed]
    [Google Scholar]
  3. Choi J., Lee S. Y. ( 1997 ). Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. . Bioprocess Eng 17, 335342. [CrossRef]
    [Google Scholar]
  4. Choi J., Lee S. Y. ( 1999 ). Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation. . Appl Microbiol Biotechnol 51, 1321. [CrossRef]
    [Google Scholar]
  5. Dias J. M. L., Lemos P. C., Serafim L. S., Oliveira C., Eiroa M., Albuquerque M. G. E., Ramos A. M., Oliveira R., Reis M. A. M. ( 2006 ). Recent advances in polyhydroxyalkanoate production by mixed aerobic cultures: from the substrate to the final product. . Macromol Biosci 6, 885906. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gorlenko V. M., Bryantseva I. A., Rabold S., Tourova T. P., Rubtsova D., Smirnova E., Thiel V., Imhoff J. F. ( 2009 ). Ectothiorhodospira variabilis sp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes. . Int J Syst Evol Microbiol 59, 658664. [CrossRef] [PubMed]
    [Google Scholar]
  7. Imhoff J. F. ( 2006 ). The family Ectothiorhodospiraceae . . In The Prokaryotes, , 3rd edn., vol. 6, pp. 874886. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Jiang Y., Marang L., Kleerebezem R., Muyzer G., van Loosdrecht M. C. M. ( 2011a ). Polyhydroxybutyrate production from lactate using a mixed microbial culture. . Biotechnol Bioeng 108, 20222035. [CrossRef] [PubMed]
    [Google Scholar]
  9. Jiang Y., Sorokin D. Y., Kleerebezem R., Muyzer G., van Loosdrecht M. C. M. ( 2011b ). Plasticicumulans acidivorans gen. nov., sp. nov., a polyhydroxyalkanoate-accumulating gammaproteobacterium from a sequencing-batch bioreactor. . Int J Syst Evol Microbiol 61, 23142319. [CrossRef] [PubMed]
    [Google Scholar]
  10. Johnson K., Jiang Y., Kleerebezem R., Muyzer G., van Loosdrecht M. C. M. ( 2009 ). Enrichment of a mixed bacterial culture with a high polyhydroxyalkanoate storage capacity. . Biomacromolecules 10, 670676. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. ( 1961 ). Procedure for isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208214. [CrossRef]
    [Google Scholar]
  14. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  15. Pohlmann A., Fricke W. F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Pötter M. et al. ( 2006 ). Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16. . Nat Biotechnol 24, 12571262. [CrossRef] [PubMed]
    [Google Scholar]
  16. Rhu D. H., Lee W. H., Kim J. Y., Choi E. ( 2003 ). Polyhydroxyalkanoate (PHA) production from waste. . Water Sci Technol 48 (8), 221228.[PubMed]
    [Google Scholar]
  17. Schroeder S., Petrovski S., Campbell B., McIlroy S., Seviour R. ( 2009 ). Phylogeny and in situ identification of a novel gammaproteobacterium in activated sludge. . FEMS Microbiol Lett 297, 157163. [CrossRef] [PubMed]
    [Google Scholar]
  18. Slater S. C., Voige W. H., Dennis D. E. ( 1988 ). Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway. . J Bacteriol 170, 44314436.[PubMed]
    [Google Scholar]
  19. Sorokin D. Y., Banciu H., van Loosdrecht M. C. M., Kuenen J. G. ( 2003 ). Growth physiology and competitive interaction of obligately chemolithoautotrophic, haloalkaliphilic, sulfur-oxidizing bacteria from soda lakes. . Extremophiles 7, 195203.[PubMed]
    [Google Scholar]
  20. Steinbüchel A. ( 2001 ). Perspectives for biotechnological production and utilization of biopolymers: metabolic engineering of polyhydroxyalkanoate biosynthesis pathways as a successful example. . Macromol Biosci 1, 124. [CrossRef]
    [Google Scholar]
  21. Takabatake H., Satoh H., Mino T., Matsuo T. ( 2000 ). Recovery of biodegradable plastics from activated sludge process. . Water Sci Technol 42 (3–4), 351356.
    [Google Scholar]
  22. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  23. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  24. van Loosdrecht M. C. M., Pot M. A., Heijnen J. J. ( 1997 ). Importance of bacterial storage polymers in bioprocesses. . Water Sci Technol 35 (1), 4147. [CrossRef]
    [Google Scholar]
  25. Vishniac W., Santer M. ( 1957 ). The thiobacilli. . Bacteriol Rev 21, 195213.[PubMed]
    [Google Scholar]
  26. Yokota A., Akagawa-Matsushita M., Hiraishi A., Katayama Y., Urakami T., Yamasato K. ( 1992 ). Distribution of quinone systems in microorganisms: Gram-negative eubacteria. . Bull Jpn Fed Culture Coll 8, 136171.
    [Google Scholar]
  27. Zhilina T. N., Zavarzin G. A., Rainey F. A., Pikuta E. N., Osipov G. A., Kostrikina N. A. ( 1997 ). Desulfonatronovibrio hydrogenovorans gen. nov., sp. nov., an alkaliphilic, sulfate-reducing bacterium. . Int J Syst Bacteriol 47, 144149. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051045-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051045-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error