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Abstract

A novel extremely halophilic archaeon KCY07-B2 was isolated from a salt mine in Kuche county, Xinjiang province, China. Colonies were cream-pigmented and cells were pleomorphic rod-shaped. Strain KCY07-B2 was able to grow at 25–50 °C (optimum 37–45 °C) and pH 6.0–8.0 (optimum 7.0). The strain required at least 1.9 M NaCl for growth. MgCl was not required. Cells lysed in distilled water. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both CC and CC glycerol diethers, together with five glyolipids. The bis-sulfated glycolipid S-DGD-1 was present. The DNA G+C content was 62.5 mol%. Analysis of the 16S rRNA gene sequence revealed that strain KCY07-B2 was closely related to SH-6 and 56 (95.8 % and 95.5 % similarity, respectively). On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KCY07-B2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KCY07-B2 ( = JCM 18547 = CGMCC 1.12284).

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2013-10-01
2019-10-14
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