1887

Abstract

An actinomycete strain, SP03-05, was isolated from a marine sponge sample ( sp.) collected from Phuket Province of Thailand. The strain was aerobic, Gram-stain-positive and produced single spores at the tips of the substrate mycelium. Strain SP03-05 contained -diaminopimelic acid in the peptidoglycan; whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. The polar lipid profile of strain SP03-05 consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unknown polar lipids. Morphological and chemotaxonomic characteristics of the strain identified it as a member of the family . Phylogenetic analysis based on 16S rRNA gene sequences showed similarity of the strain to YIM 013 (96.90 %), MS426 (96.90 %), DSM 44337 (96.80 %), AB-18-032 (96.80 %) and RtIII47 (95.40 %). The DNA G+C content was 72.4 mol%. The phenotypic, genotypic and DNA–DNA hybridization results supported the classification of this strain as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SP03-05 ( = BCC 45620 = NBRC 109075).

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2013-11-01
2019-12-13
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