1887

Abstract

An actinomycete strain, SP03-05, was isolated from a marine sponge sample ( sp.) collected from Phuket Province of Thailand. The strain was aerobic, Gram-stain-positive and produced single spores at the tips of the substrate mycelium. Strain SP03-05 contained -diaminopimelic acid in the peptidoglycan; whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. The polar lipid profile of strain SP03-05 consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unknown polar lipids. Morphological and chemotaxonomic characteristics of the strain identified it as a member of the family . Phylogenetic analysis based on 16S rRNA gene sequences showed similarity of the strain to YIM 013 (96.90 %), MS426 (96.90 %), DSM 44337 (96.80 %), AB-18-032 (96.80 %) and RtIII47 (95.40 %). The DNA G+C content was 72.4 mol%. The phenotypic, genotypic and DNA–DNA hybridization results supported the classification of this strain as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SP03-05 ( = BCC 45620 = NBRC 109075).

Funding
This study was supported by the:
  • Thailand Graduate Institute of Science and Technology, National Science and Technology Development Agency (NSTDA) (Award TGIST-01-52-003)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.050906-0
2013-11-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/3970.html?itemId=/content/journal/ijsem/10.1099/ijs.0.050906-0&mimeType=html&fmt=ahah

References

  1. Arai T. ( 1975 ). Culuture Media for Actinomycetes. Tokyo:: The Society for Actinomycetes Japan;.
    [Google Scholar]
  2. Bister B., Bischoff D., Ströbele M., Riedlinger J., Reicke A., Wolter F., Bull A. T., Zähner H., Fiedler H. P., Süssmuth R. D. ( 2004 ). Abyssomicin C–A polycyclic antibiotic from a marine Verrucosispora strain as an inhibitor of the p-aminobenzoic acid/tetrahydrofolate biosynthesis pathway. . Angew Chem Int Ed Engl 43, 25742576. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  4. Dai H. Q., Wang J., Xin Y. H., Pei G., Tang S. K., Ren B., Ward A., Ruan J. S., Li W. J., Zhang L. X. ( 2010 ). Verrucosispora sediminis sp. nov., a cyclodipeptide-producing actinomycete from deep-sea sediment. . Int J Syst Evol Microbiol 60, 18071812. [View Article] [PubMed]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Fiedler H. P., Bruntner C., Riedlinger J., Bull A. T., Knutsen G., Goodfellow M., Jones A., Maldonado L., Pathom-aree W. & other authors ( 2008 ). Proximicin A, B and C, novel aminofuran antibiotic and anticancer compounds isolated from marine strains of the actinomycete Verrucosispora . . J Antibiot (Tokyo) 61, 158163. [View Article] [PubMed]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a species tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  10. Goodfellow M., Stach J. E., Brown R., Bonda A. N., Jones A. L., Mexson J., Fiedler H. P., Zucchi T. D., Bull A. T. ( 2012 ). Verrucosispora maris sp. nov., a novel deep-sea actinomycete isolated from a marine sediment which produces abyssomicins. . Antonie van Leeuwenhoek 101, 185193. [View Article] [PubMed]
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [View Article]
    [Google Scholar]
  12. Itoh T., Kudo T., Parenti F., Seino A. ( 1989 ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. . Int J Syst Bacteriol 39, 168173. [View Article]
    [Google Scholar]
  13. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  14. Kawamoto I., Oka T., Nara T. ( 1981 ). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146, 527534.[PubMed]
    [Google Scholar]
  15. Keller S., Nicholson G., Drahl C., Sorensen E., Fiedler H. P., Süssmuth R. D. ( 2007 ). Abyssomicins G and H and atrop-abyssomicin C from the marine Verrucosispora strain AB-18-032. . J Antibiot (Tokyo) 60, 391394. [View Article] [PubMed]
    [Google Scholar]
  16. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standard Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  18. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  19. Komagata K., Suzuki K. I. ( 1988 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  20. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  21. Liao Z. L., Tang S. K., Guo L., Zhang Y. Q., Tian X. P., Jiang C. L., Xu L. H., Li W. J. ( 2009 ). Verrucosispora lutea sp. nov., isolated from a mangrove sediment sample. . Int J Syst Evol Microbiol 59, 22692273. [View Article] [PubMed]
    [Google Scholar]
  22. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  23. Rheims H., Schumann P., Rohde M., Stackebrandt E. ( 1998 ). Verrucosispora gifhornensis gen. nov., sp. nov., a new member of the actinobacterial family Micromonosporaceae . . Int J Syst Bacteriol 48, 11191127. [View Article] [PubMed]
    [Google Scholar]
  24. Riedlinger J., Reicke A., Zähner H., Krismer B., Bull A. T., Maldonado L. A., Ward A. C., Goodfellow M., Bister B. & other authors ( 2004 ). Abyssomicins, inhibitors of the para-aminobenzoic acid pathway produced by the marine Verrucosispora strain AB-18-032. . J Antibiot (Tokyo) 57, 271279. [View Article] [PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  27. Schneider K., Keller S., Wolter F. E., Röglin L., Beil W., Seitz O., Nicholson G., Bruntner C., Riedlinger J. & other authors ( 2008 ). Proximicins A, B, and C-antitumor furan analogues of netropsin from the marine actinomycete Verrucosispora induce upregulation of p53 and the cyclin kinase inhibitor p21. . Angew Chem Int Ed Engl 47, 32583261. [View Article] [PubMed]
    [Google Scholar]
  28. Shirai M., Okuda M., Motohashi K., Imoto M., Furihata K., Matsuo Y., Katsuta A., Shizuri Y., Seto H. ( 2010 ). Terpenoids produced by actinomycetes: isolation, structural elucidation and biosynthesis of new diterpenes, gifhornenolones A and B from Verrucosispora gifhornensis YM28-088. . J Antibiot (Tokyo) 63, 245250. [View Article] [PubMed]
    [Google Scholar]
  29. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [View Article]
    [Google Scholar]
  30. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  31. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J. J. ( 2009 ). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. . Int J Syst Evol Microbiol 59, 992997. [View Article] [PubMed]
    [Google Scholar]
  32. Tamaoka J. ( 1994 ). Determination of DNA base composition. . In Chemical Methods in Prokaryotic Systematics, pp. 463470. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  33. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  34. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  35. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  36. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  37. Uchida K., Aida K. ( 1984 ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol 30, 131134. [View Article]
    [Google Scholar]
  38. Verlander C. P. ( 1992 ). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185201. Edited by Kricka L. J. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  39. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  40. Williams S. T., Cross T. ( 1971 ). Actinomycetes. . In Methods in Microbiology, vol. 4, pp. 295334. Edited by Booth C. . London:: Academic Press;.
    [Google Scholar]
  41. Wyche T. P., Hou Y., Braun D., Cohen H. C., Xiong M. P., Bugni T. S. ( 2011 ). First natural analogs of the cytotoxic thiodepsipeptide thiocoraline A from a marine Verrucosispora sp.. J Org Chem 76, 65426547. [View Article] [PubMed]
    [Google Scholar]
  42. Xi L., Zhang L., Ruan J., Huang Y. ( 2012 ). Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora . . Int J Syst Evol Microbiol 62, 15641569. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.050906-0
Loading
/content/journal/ijsem/10.1099/ijs.0.050906-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error