1887

Abstract

Two strictly aerobic, extremely halophilic archaea, strains WSY15-H1 and WSY15-H3, were isolated from a salt mine in Wensu county, Xinjiang province, China. Cells of the two strains were Gram-stain-negative, non-motile and pleomorphic. Colonies were pink- and red-pigmented, respectively. Strain WSY15-H1 grew at 20–45 °C (optimum 37–42 °C), 1.6–5.4 M NaCl (optimum 3.4–3.9 M), 0–2.0 M MgCl (optimum 0.1–0.5 M) and pH 6.0–9.0 (optimum 7.0), whereas strain WSY15-H3 grew at 20–50 °C (optimum 37 °C), 1.9–5.4 M NaCl (optimum 3.4 M), 0.02–2.5 M MgCl (optimum 0.5-1.0 M) and pH 6.0–7.5 (optimum 6.5). The minimal NaCl concentrations to prevent cell lysis were 9 % (w/v) for strain WSY15-H1 and 8 % (w/v) for strain WSY15-H3. The major polar lipids of the two isolates were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate, as well as nine glycolipids for strain WSY15-H1 and seven glycolipids for strain WSY15-H3; two of these glycolipids (GL1 and GL3) were chromatographically identical to bis-sulfated diglycosyl diether (S-DGD-1) and sulfated diglycosyl diether (S-DGD-1), respectively. The genomic DNA G+C contents of strains WSY15-H1 and WSY15-H3 were 65.4 and 66.2 mol%. On the basis of 16S rRNA gene sequence analysis, strains WSY15-H1 and WSY15-H3 shared 97.0 % similarity with each other and showed respectively 98.4 and 97.6 % sequence similarity to TBN21, which was the only type strain that had higher than 91 % sequence similarity with the two isolates. Analysis of phylogenetic relationships and DNA–DNA relatedness indicated that strains WSY15-H1 and WSY15-H3 represent two novel lineages with closest affinity to TBN21. Based on phenotypic, chemotaxonomic and genotypic characteristics, two novel species of the genus are proposed, sp. nov. (type strain WSY15-H1 = JCM 18548 = GCMCC 1.12371) and sp. nov. (type strain WSY15-H3 = JCM 18549 = GCMCC 1.12285).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 30970002 and 31170001)
  • , key Laboratory project of Xinjiang Academy of Sciences , (Award LBB-2011-005)
  • , University-Industry cooperation project of Hangzhou city , (Award 201112731E111)
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2013-12-01
2020-08-14
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