1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_9

sp. nov., an algicidal bacterium isolated from coastal seawater Free

Abstract

A Gram-stain-negative, elongated rod-shaped, yellow-pigmented, aerobic bacterial strain, designated WJ-1, was isolated from coastal seawater in Xiamen, Fujian province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain WJ-1 fell within the genus and was most closely associated with SMK-4 (96.7 % 16S rRNA gene sequence similarity); lower similarities were shown to other members of the genus (<96.2 %). The strain formed a distinct lineage with B-I (96.0 %), YCS-6 (94.5 %) and CNURIC 013 (95.4 %). Growth was observed at temperatures from 16 to 38 °C, at salinities from 2 to 4 % and at pH 6–9. The major fatty acids were summed feature 3 (Cω6 and/or Cω7), iso-C 3-OH, iso-C and iso-C 3-OH. The DNA G+C content of strain WJ-1 was 33.2 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Differential phenotypic properties and phylogenetic distinctiveness in this study distinguished strain WJ-1 from all other members of the genus . According to the morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain WJ-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WJ-1 ( = CGMCC 1.12378 = LMG 27422).

  • Published Online:
Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 40930847)
  • Special Fund for PhD Program in University (Award 20120121130001)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201305022, 201305041 and 201305016)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.050765-0
2013-09-01
2023-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3481.html?itemId=/content/journal/ijsem/10.1099/ijs.0.050765-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) ( 1995 ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
     [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  3. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  4. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 5685–5689. [View Article] [PubMed]
    [Google Scholar]
  5. Hansen G. H., Bergh Ø., Michaelsen J., Knappskog D. ( 1992 ). Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. . Int J Syst Bacteriol 42, 451–458. [View Article] [PubMed]
    [Google Scholar]
  6. Jung S. Y., Oh T. K., Yoon J. H. ( 2006 ). Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. . Int J Syst Evol Microbiol 56, 1577–1581. [View Article] [PubMed]
    [Google Scholar]
  7. Kang S. J., Lee S. Y., Lee M. H., Oh T. K., Yoon J. H. ( 2012 ). Tenacibaculum geojense sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62, 18–22. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  9. Li Y., Bai S., Yang C., Lai Q., Zhang H., Chen Z., Wei J., Zheng W., Tian Y., Zheng T. ( 2013 ). Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 63, 2043–2048. [View Article] [PubMed]
    [Google Scholar]
  10. Oh Y. S., Kahng H. Y., Lee D. H., Lee S. B. ( 2012 ). Tenacibaculum jejuense sp. nov., isolated from coastal seawater. . Int J Syst Evol Microbiol 62, 414–419. [View Article] [PubMed]
    [Google Scholar]
  11. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  12. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  13. Sheu S. Y., Lin K. Y., Chou J. H., Chang P. S., Arun A. B., Young C. C., Chen W. M. ( 2007 ). Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. . Int J Syst Evol Microbiol 57, 1148–1153. [View Article] [PubMed]
    [Google Scholar]
  14. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 2001 ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51, 1639–1652. [View Article] [PubMed]
    [Google Scholar]
  15. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  16. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  17. Wakabayashi H., Hikida M., Masumura K. ( 1986 ). Flexibacter maritimus sp. nov., a pathogen of marine fishes. . Int J Syst Bacteriol 36, 396–398. [View Article]
    [Google Scholar]
  18. Yang C., Li Y., Zhou Y., Zheng W., Tian Y., Zheng T. ( 2012 ). Bacterial community dynamics during a bloom caused by Akashiwo sanguinea in the Xiamen sea area, China. . Harmful Algae 20, 132–141. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.050765-0
Loading
Tenacibaculumxiamenense sp. nov., an algicidal bacterium isolated from coastal seawater
Int J Syst Evol Microbiol 63, 3481 (2013); https://doi.org/10.1099/ijs.0.050765-0
/content/journal/ijsem/10.1099/ijs.0.050765-0
/content/journal/ijsem/10.1099/ijs.0.050765-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error