1887

Abstract

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1033, was isolated from soil and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1033 formed a distinct lineage within the genus and was most closely related to KCTC 13025 (similarity 95.9 %). CAU 1033 contained MK-7 as the only isoprenoid quinone and iso-C and anteiso-C as the major fatty acids. The cell wall peptidoglycan of strain CAU 1033 contained -diaminopimelic acid and the major whole-cell sugars were arabinose, sucrose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, four unidentified aminophospholipids, an unidentified aminolipid, two unidentified glycolipids and another unidentified polar lipid. The DNA G+C content was 41.4 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1033 was classified as a representative of a novel species in the genus for which the name sp. nov. is proposed. The type strain is CAU 1033 ( = KCTC 13881 = CCUG 61889).

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2013-11-01
2024-03-28
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References

  1. Ahmed I., Yokota A., Fujiwara T. ( 2007a ). A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth. . Extremophiles 11, 217224. [View Article] [PubMed]
    [Google Scholar]
  2. Ahmed I., Yokota A., Yamazoe A., Fujiwara T. ( 2007b ). Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.. Int J Syst Evol Microbiol 57, 11171125. [View Article] [PubMed]
    [Google Scholar]
  3. Albert R. A., Archambault J., Lempa M., Hurst B., Richardson C., Gruenloh S., Duran M., Worliczek H. L., Huber B. E. & other authors ( 2007 ). Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.. Int J Syst Evol Microbiol 57, 27292737. [View Article] [PubMed]
    [Google Scholar]
  4. Arfman N., Dijkhuizen L., Kirchhof G., Ludwig W., Schleifer K. H., Bulygina E. S., Chumakov K. M., Govorukhina N. I., Trotsenko Y. A. & other authors ( 1992 ). Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria. . Int J Syst Bacteriol 42, 439445. [View Article] [PubMed]
    [Google Scholar]
  5. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. ( 1991 ). Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit ribosomal-RNA sequences. . Lett Appl Microbiol 13, 202206. [View Article]
    [Google Scholar]
  6. Claus D., Berkeley R. C. W. ( 1986 ). Genus Bacillus Cohn 1872. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 11051139. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  7. Conn H. J., Bartholomew J. W., Jennison M. W. ( 1957 ). Staining methods. . In Manual of Microbial Methods, pp. 3036. Edited by the Society of American Bacteriologists . New York:: McGraw-Hill;.
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  10. Felsenstein J. ( 1989 ). phylip – phylogeny inference package (version 3.2). . Cladistics 5, 164166.
    [Google Scholar]
  11. Fitch W. M., Margoliash E. ( 1967 ). Construction of phylogenetic trees. . Science 155, 279284. [View Article] [PubMed]
    [Google Scholar]
  12. Gordon R. E., Mihm J. M. ( 1962 ). Identification of Nocardia caviae (Erikson) nov. comb.. Ann N Y Acad Sci 98, 628636. [View Article]
    [Google Scholar]
  13. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P. ( 2004 ). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. . Int J Syst Evol Microbiol 54, 4757. [View Article] [PubMed]
    [Google Scholar]
  14. Heyrman J., Rodríguez-Díaz M., Devos J., Felske A., Logan N. A., De Vos P. ( 2005 ). Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. . Int J Syst Evol Microbiol 55, 111117. [View Article] [PubMed]
    [Google Scholar]
  15. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. H. . New York:: Academic Press;.
    [Google Scholar]
  16. Kämpfer P. ( 1994 ). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. . Syst Appl Microbiol 17, 8698. [View Article]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  18. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161208. [View Article]
    [Google Scholar]
  19. Lányi B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  20. Lim J.-M., Jeon C. O., Kim C.-J. ( 2006 ). Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. . Int J Syst Evol Microbiol 56, 29032908. [View Article] [PubMed]
    [Google Scholar]
  21. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  22. Minnikin D. E., Goodfellow M. ( 1981 ). Lipids in the classification of Bacillus and related taxa. . In The Aerobic Endospore-Forming Bacteria ( Special Publication of the Society for General Microbiology no. 4), pp. 5990. Edited by Berkeley R. C. W., Goodfellow M. . London:: Academic Press;.
    [Google Scholar]
  23. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [View Article]
    [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  25. Nam S. W., Kim W., Chun J., Goodfellow M. ( 2004 ). Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. . Int J Syst Evol Microbiol 54, 12091212. [View Article] [PubMed]
    [Google Scholar]
  26. Nicholson W. L., Setlow P. ( 1990 ). Sporulation, germination and outgrowth. . In Molecular Biological Methods for Bacillus, pp. 391450. Edited by Harwood C. R., Cutting S. M. . Chichester:: Wiley;.
    [Google Scholar]
  27. Priest F. G., Goodfellow M., Todd C. ( 1981 ). The genus Bacillus: a numerical analysis. . In The Aerobic Endospore-Forming Bacteria. Classification and Identification, pp. 91103. Edited by Berkeley R. C. W., Goodfellow M. . London:: Academic Press;.
    [Google Scholar]
  28. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  29. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123156. [View Article]
    [Google Scholar]
  30. Shaw S., Keddie R. M. ( 1983 ). A numerical taxonomic study of the genus Kurthia with a revised description of Kurthia zopfii and a description of Kurthia gibsonii sp. nov.. Syst Appl Microbiol 4, 253276. [View Article] [PubMed]
    [Google Scholar]
  31. Slepecky R., Hemphill E. ( 1992 ). The genus Bacillus – nonmedical. . In The Prokaryotes, , 2nd edn., pp. 16631696. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;.
    [Google Scholar]
  32. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  33. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  35. Vaishampayan P., Miyashita M., Ohnishi A., Satomi M., Rooney A., La Duc M. T., Venkateswaran K. ( 2009 ). Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura et al. 2002 as Rummeliibacillus pycnus comb. nov.. Int J Syst Evol Microbiol 59, 10941099. [View Article] [PubMed]
    [Google Scholar]
  36. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982 ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128, 19591968.
    [Google Scholar]
  37. Wieser M., Worliczek H., Kämpfer P., Busse H. J. ( 2005 ). Bacillus herbersteinensis sp. nov.. Int J Syst Evol Microbiol 55, 21192123. [View Article] [PubMed]
    [Google Scholar]
  38. Yoon J.-H., Kim I.-G., Kang K. H., Oh T.-K., Park Y.-H. ( 2003 ). Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 53, 12971303. [View Article] [PubMed]
    [Google Scholar]
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