1887

Abstract

Two novel isolates of rapidly growing, Gram-stain-positive, non-chromogenic species of the genus , strain YIM M13028 from a sediment sample collected from the South China Sea (19° 30.261′ N 111° 0.247′ E) at a depth of 42 m and strain YIM 121001 from a coastal zone sand sample collected in Dubai, United Arab Emirates, were obtained in our laboratory. Their taxonomic positions were determined by a polyphasic approach. Good growth of the two strains was observed at 28 °C and pH 7.0 with 0–2 % NaCl on tryptic soy agar medium. Both strains formed round orange–red colonies, strain YIM M13028 had a rough surface, while YIM 121001 was smooth. Cellular fatty acids, whole-cell protein profiles and TLC analysis of their mycolic acids show significant differences from reference stains. Phenotypic characteristics and multilocus sequence analysis (MLSA) of 16S rRNA gene, and 16S–23S internal transcribed spacer (ITS) sequences indicated that both strains YIM M13028 and YIM 121001 belong to the genus . DNA–DNA hybridization values revealed a low relatedness (<70 %) of the two isolates with the type strains DSM 44074 and DSM 44183. The low DNA–DNA hybridization values (40.4±3.5 %) between strains YIM M13028 and YIM 121001 and phenotypic distinctiveness indicated that the two strains were representatives of different novel species of the genus . The names proposed for these novel species are sp. nov. and sp. nov., and the type strains are YIM M13028 ( = DSM 45643 = KCTC 19999) and YIM 121001 ( = DSM 45768 = JCM 18538), respectively.

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2013-11-01
2020-01-20
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