1887

Abstract

A novel moderately thermophilic, heterotrophic bacterium was isolated from a deep-sea hydrothermal vent deposit from the Mariner field along the Eastern Lau Spreading Center of the south-western Pacific Ocean. Cells were short motile rods (about 0.4×0.8 µm) that occurred singly or in pairs and were surrounded by a sheath-like membrane or ‘toga’. The cells grew between 45 and 65 °C (optimum 57–60 °C) and at pH 4.1–6.0 (optimum pH 5.5–5.7) and grew optimally at 3 % (w/v) NaCl. The isolate grew on a range of carbon and proteinaceous substrates and reduced sulfur. The G+C content of the DNA was about 45 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the new isolate as a deeply diverging lineage within the order . Based on the physiological, morphological and phylogenetic data, the isolate represents a novel species of a new genus with the proposed name gen. nov., sp. nov. The type strain of is cd-1655R ( = DSM 25116 = OCM 1212).

Funding
This study was supported by the:
  • , US National Science Foundation , (Award OCE-0937404 and OCE-0752469)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.050518-0
2013-12-01
2020-08-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4724.html?itemId=/content/journal/ijsem/10.1099/ijs.0.050518-0&mimeType=html&fmt=ahah

References

  1. Alain K., Marteinsson V. T., Miroshnichenko M. L., Bonch-Osmolovskaya E. A., Prieur D., Birrien J. L. ( 2002 ). Marinitoga piezophila sp. nov., a rod-shaped, thermo-piezophilic bacterium isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 52, 13311339. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [CrossRef] [PubMed]
    [Google Scholar]
  3. Andrews K. T., Patel B. K. C. ( 1996 ). Fervidobacterium gondwanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothermal waters of the Great Artesian Basin of Australia. . Int J Syst Bacteriol 46, 265269. [CrossRef] [PubMed]
    [Google Scholar]
  4. Antoine E., Cilia V., Meunier J. R., Guezennec J., Lesongeur F., Barbier G. ( 1997 ). Thermosipho melanesiensis sp. nov., a new thermophilic anaerobic bacterium belonging to the order Thermotogales, isolated from deep-sea hydrothermal vents in the southwestern Pacific Ocean. . Int J Syst Bacteriol 47, 11181123. [CrossRef] [PubMed]
    [Google Scholar]
  5. Ben Hania W., Ghodbane R., Postec A., Brochier-Armanet C., Hamdi M., Fardeau M.-L., Ollivier B. ( 2011 ). Cultivation of the first mesophilic representative (“mesotoga”) within the order Thermotogales . . Syst Appl Microbiol 34, 581585. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ben Hania W., Godbane R., Postec A., Hamdi M., Ollivier B., Fardeau M.-L. ( 2012 ). Defluviitoga tunisiensis gen. nov., sp. nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester. . Int J Syst Evol Microbiol 62, 13771382. [CrossRef] [PubMed]
    [Google Scholar]
  7. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [CrossRef] [PubMed]
    [Google Scholar]
  8. DiPippo J. L., Nesbø C. L., Dahle H., Doolittle W. F., Birkland N.-K., Noll K. M. ( 2009 ). Kosmotoga olearia gen. nov., sp. nov., a thermophilic, anaerobic heterotroph isolated from an oil production fluid. . Int J Syst Evol Microbiol 59, 29913000. [CrossRef] [PubMed]
    [Google Scholar]
  9. Flores G. E., Campbell J. H., Kirshtein J. D., Meneghin J., Podar M., Steinberg J. I., Seewald J. S., Tivey M. K., Voytek M. A. & other authors ( 2011 ). Microbial community structure of hydrothermal deposits from geochemically different vent fields along the Mid-Atlantic Ridge. . Environ Microbiol 13, 21582171. [CrossRef] [PubMed]
    [Google Scholar]
  10. Flores G. E., Shakya M., Meneghin J., Yang Z. K., Seewald J. S., Geoff Wheat C., Podar M., Reysenbach A.-L. ( 2012 ). Inter-field variability in the microbial communities of hydrothermal vent deposits from a back-arc basin. . Geobiology 10, 333346. [CrossRef] [PubMed]
    [Google Scholar]
  11. Götz D., Banta A., Beveridge T. J., Rushdi A. I., Simoneit B. R., Reysenbach A.-L. ( 2002 ). Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents. . Int J Syst Evol Microbiol 52, 13491359. [CrossRef] [PubMed]
    [Google Scholar]
  12. Inagaki F., Kuypers M. M. M., Tsunogai U., Ishibashi J., Nakamura K., Treude T., Ohkubo S., Nakaseama M., Gena K. & other authors ( 2006 ). Microbial community in a sediment-hosted CO2 lake of the southern Okinawa Trough hydrothermal system. . Proc Natl Acad Sci U S A 103, 1416414169. [CrossRef] [PubMed]
    [Google Scholar]
  13. Jayasinghearachchi H. S., Lal B. ( 2011 ). Oceanotoga teriensis gen. nov., sp. nov., a thermophilic bacterium isolated from offshore oil-producing wells. . Int J Syst Evol Microbiol 61, 554560. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kato S., Takano Y., Kakegawa T., Oba H., Inoue K., Kobayashi C., Utsumi M., Marumo K., Kobayashi K. & other authors ( 2010 ). Biogeography and biodiversity in sulfide structures of active and inactive vents at deep-sea hydrothermal fields of the Southern Mariana Trough. . Appl Environ Microbiol 76, 29682979. [CrossRef] [PubMed]
    [Google Scholar]
  15. L’Haridon S., Miroshnichenko M. L., Hippe H., Fardeau M.-L., Bonch-Osmolovskaya E. A., Stackebrandt E., Jeanthon C. ( 2002 ). Petrotoga olearia sp. nov. and Petrotoga sibirica sp. nov., two thermophilic bacteria isolated from a continental petroleum reservoir in Western Siberia. . Int J Syst Evol Microbiol 52, 17151722. [CrossRef] [PubMed]
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  17. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  18. Nesbø C. L., Dlutek M., Zhaxybayeva O., Doolittle W. F. ( 2006 ). Evidence for existence of “mesotogas,” members of the order Thermotogales adapted to low-temperature environments. . Appl Environ Microbiol 72, 50615068. [CrossRef] [PubMed]
    [Google Scholar]
  19. Nesbø C. L., Kumaraswamy R., Dlutek M., Doolittle W. F., Foght J. ( 2010 ). Searching for mesophilic Thermotogales bacteria: “mesotogas” in the wild. . Appl Environ Microbiol 76, 48964900. [CrossRef] [PubMed]
    [Google Scholar]
  20. Nesbø C. L., Bradnan D. M., Adebusuyi A., Dlutek M., Petrus A. K., Foght J., Doolittle W. F., Noll K. M. ( 2012 ). Mesotoga prima gen. nov., sp. nov., the first described mesophilic species of the Thermotogales . . Extremophiles 16, 387393. [CrossRef] [PubMed]
    [Google Scholar]
  21. Nishida H., Beppu T., Ueda K. ( 2011 ). Whole-genome comparison clarifies close phylogenetic relationships between the phyla Dictyoglomi and Thermotogae . . Genomics 98, 370375. [CrossRef] [PubMed]
    [Google Scholar]
  22. Nunoura T., Oida H., Miyazaki M., Suzuki Y., Takai K., Horikoshi K. ( 2007 ). Marinitoga okinawensis sp. nov., a novel thermophilic and anaerobic heterotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough. . Int J Syst Evol Microbiol 57, 467471. [CrossRef] [PubMed]
    [Google Scholar]
  23. Nunoura T., Hirai M., Imachi H., Miyazaki M., Makita H., Hirayama H., Furushima Y., Yamamoto H., Takai K. ( 2010 ). Kosmotoga arenicorallina sp. nov. a thermophilic and obligately anaerobic heterotroph isolated from a shallow hydrothermal system occurring within a coral reef, southern part of the Yaeyama Archipelago, Japan, reclassification of Thermococcoides shengliensis as Kosmotoga shengliensis comb. nov., and emended description of the genus Kosmotoga . . Arch Microbiol 192, 811819. [CrossRef] [PubMed]
    [Google Scholar]
  24. Postec A., Le Breton C., Fardeau M.-L., Lesongeur F., Pignet P., Querellou J., Ollivier B., Godfroy A. ( 2005 ). Marinitoga hydrogenitolerans sp. nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge. . Int J Syst Evol Microbiol 55, 12171221. [CrossRef] [PubMed]
    [Google Scholar]
  25. Postec A., Ciobanu M., Birrien J.-L., Bienvenu N., Prieur D., Le Romancer M. ( 2010 ). Marinitoga litoralis sp. nov., a thermophilic, heterotrophic bacterium isolated from a coastal thermal spring on Ile Saint-Paul, Southern Indian Ocean. . Int J Syst Evol Microbiol 60, 17781782. [CrossRef] [PubMed]
    [Google Scholar]
  26. Ravot G., Ollivier B., Magot M., Patel B., Crolet J., Fardeau M., Garcia J. ( 1995 ). Thiosulfate reduction, an important physiological feature shared by members of the order Thermotogales . . Appl Environ Microbiol 61, 20532055.[PubMed]
    [Google Scholar]
  27. Reysenbach A.-L., Liu Y., Banta A. B., Beveridge T. J., Kirshtein J. D., Schouten S., Tivey M. K., Von Damm K. L., Voytek M. A. ( 2006 ). A ubiquitous thermoacidophilic archaeon from deep-sea hydrothermal vents. . Nature 442, 444447. [CrossRef] [PubMed]
    [Google Scholar]
  28. Sinninghe Damsté J. S., Rijpstra W. I. C., Hopmans E. C., Schouten S., Balk M., Stams A. J. ( 2007 ). Structural characterization of diabolic acid-based tetraester, tetraether and mixed ether/ester, membrane-spanning lipids of bacteria from the order Thermotogales . . Arch Microbiol 188, 629641. [CrossRef] [PubMed]
    [Google Scholar]
  29. Stamatakis A., Hoover P., Rougemont J. ( 2008 ). A rapid bootstrap algorithm for the RAxML Web servers. . Syst Biol 57, 758771. [CrossRef] [PubMed]
    [Google Scholar]
  30. Stott M. B., Saito J. A., Crowe M. A., Dunfield P. F., Hou S., Nakasone E., Daughney C. J., Smirnova A. V., Mountain B. W. & other authors ( 2008 ). Culture-independent characterization of a novel microbial community at a hydrothermal vent at Brothers volcano, Kermadec arc, New Zealand. . J Geophys Res 113 (B8), B08S06. [CrossRef]
    [Google Scholar]
  31. Takai K., Horikoshi K. ( 2000 ). Thermosipho japonicus sp. nov., an extremely thermophilic bacterium isolated from a deep-sea hydrothermal vent in Japan. . Extremophiles 4, 917.[PubMed] [CrossRef]
    [Google Scholar]
  32. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  33. Urios L., Cueff-Gauchard V., Pignet P., Postec A., Fardeau M.-L., Ollivier B., Barbier G. ( 2004 ). Thermosipho atlanticus sp. nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent. . Int J Syst Evol Microbiol 54, 19531957. [CrossRef] [PubMed]
    [Google Scholar]
  34. Wery N., Lesongeur F., Pignet P., Derennes V., Cambon-Bonavita M. A., Godfroy A., Barbier G. ( 2001 ). Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 51, 495504. [CrossRef] [PubMed]
    [Google Scholar]
  35. Wilson K. ( 1997 ). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 2.4.12.4.5. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. , Struhl K. . New York:: Greene Publishing and Wiley-Interscience;.
    [Google Scholar]
  36. Zhaxybayeva O., Swithers K. S., Lapierre P., Fournier G. P., Bickhart D. M., DeBoy R. T., Nelson K. E., Nesbø C. L., Doolittle W. F. & other authors ( 2009 ). On the chimeric nature, thermophilic origin, and phylogenetic placement of the Thermotogales . . Proc Natl Acad Sci U S A 106, 58655870. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.050518-0
Loading
/content/journal/ijsem/10.1099/ijs.0.050518-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error