RT Journal Article SR Electronic(1) A1 Liu, Hongliang A1 Song, Yumei A1 Chen, Fang A1 Zheng, Shixue A1 Wang, GejiaoYR 2013 T1 Lysinibacillusmanganicus sp. nov., isolated from manganese mining soil JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_10 SP 3568 OP 3573 DO https://doi.org/10.1099/ijs.0.050492-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, aerobic, motile, rod-shaped bacterium, designated strain Mn1-7T, was isolated from manganese mining soil in Tianjin, China. The closest phylogenetic relatives were Lysinibacillus massiliensis CCUG 49529T (97.2 % 16S rRNA gene sequence similarity), L. xylanilyticus XDB9T (96.7 %), L. sinduriensis JCM 15800T (96.2 %), L. odysseyi NBRC 100172T (95.9 %) and L. boronitolerans NBRC 103108T (95.4 %) (the type species of the genus). DNA–DNA hybridization values for strain Mn1-7T with the type strains of L. massiliensis and L. sinduriensis were 24.9 and 27.7 %, respectively. The genomic DNA G+C content was 38.4 mol%. The major menaquinone was MK-7 and the major fatty acids were iso-C15 : 0, iso-C16 : 0 and iso-C14 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was type A4α (l-Lys–d-Asp), and the predominant cell-wall sugar was xylose. DNA–DNA hybridization results and comparison of phenotypic and chemotaxonomic characters between strain Mn1-7T and the phylogenetically most closely related strains revealed that the isolate represents a novel species of the genus Lysinibacillus , for which the name Lysinibacillus manganicus sp. nov. is proposed. The type strain is Mn1-7T ( = DSM 26584T = CCTCC AB 2012916T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.050492-0