1887

Abstract

A hydrogenogenic, carboxydotrophic marine bacterium, strain KKC1, was isolated from a sediment core sample taken from a submerged marine caldera. Cells were non-motile, Gram-stain-negative, 1.0–3.0 µm straight rods, often observed with round endospores. Strain KKC1 grew at 55–68 °C, pH 5.2–9.2 and 0.8–14 % (w/v) salinity. Optimum growth occurred at 65 °C, pH 7.0–7.5 and 2.46 % salinity with a doubling time of 3.7 h. The isolate grew chemolithotrophically, producing H from carbon monoxide (CO) oxidation with reduction of various electron acceptors, e.g. sulfite, thiosulfate, fumarate, ferric iron and AQDS (9,10-anthraquinone 2,6-disulfonate). KKC1 grew heterotrophically on pyruvate, lactate, fumarate, glucose, fructose and mannose with thiosulfate as an electron acceptor. When grown mixotrophically on CO and pyruvate, C constituted almost half of the total cellular fatty acids. The DNA G+C content was 50.6 mol%. The 16S rRNA gene sequence of KKC1 was most closely related to those of members of the genus with similarity ranging from 91 to 89 %. Based on physiological and phylogenetic novelty, we propose the isolate as a representative of a new genus and novel species with the name gen. nov., sp. nov.; the type strain of the type species is KKC1 ( = DSM 26464 = NBRC 109353).

Funding
This study was supported by the:
  • Geo Biotechnology Development Organization
  • Ministry of Education, Culture, Sports, Science and Technology (MEXT) (Award 20248023)
  • Japan Society for the Promotion of Science (JSPS) (Award 244441)
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2013-10-01
2021-10-22
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