1887

Abstract

A bacterial strain, designated PECAE04, was isolated from root nodules of in Spain. Phylogenetic analysis based on 16S rRNA gene sequence placed the isolate into the genus with its closest relative being Ch-32 with 98.4 % 16S rRNA gene sequence similarity followed by DS-1, PW21, B538 and D75 with similarities ranging from 97.9 to 96.8 %. DNA–DNA hybridization measurements showed values lower than 20 % between the strain PECAE04 and any of these species. The isolate was a Gram-stain-positive, motile, sporulating rod. Catalase and oxidase activities were positive. Aesculin was hydrolysed but casein and gelatin were not. Acetoin production, HS production, nitrate reduction and urease and caseinase production were negative. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the predominant menaquinone and anteiso-C, iso-C and C were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, a glycolipid, three phospholipids and an unidentified lipid. -diaminopimelic acid was not detected in the peptidoglycan. The DNA G+C content was 52.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain PECAE04 should be considered to be a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PECAE04 ( = LMG 27297 = CECT 8234).

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2013-12-01
2019-12-06
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