1887

Abstract

Strain MBR was isolated from landfill leachate in a solid-waste disposal site in Chengdu, Sichuan, China. An analysis of 16S rRNA gene sequences revealed that the isolate was closely related to members of the genus , sharing the highest sequence similarities with HT-3 (99.8 %), AL15-21 (99.7 %) and ATCC 8062 (99.4 %). Multi-locus sequence analysis based on three housekeeping genes (, and ) provided higher resolution at the species level than that based on 16S rRNA gene sequences, which was further confirmed by less than 70 % DNA–DNA relatedness between the new isolate and HT-3 (61.3 %), AL15-21 (51.5 %) and ATCC 8062 (57.8 %). The DNA G+C content of strain MBR was 61.9 mol% and the major ubiquinone was Q-9. The major cellular fatty acids (>10 %) were Cω7 and/or Cω6, C, and Cω7 and/or Cω6. Polyphasic analysis indicates that strain MBR represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MBR ( = CGMCC 2318 = DSM 26382).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31270531)
  • West Light Foundation of the Chinese Academy of Sciences
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2014-01-01
2024-03-29
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References

  1. Carlson C. A., Ingraham J. L. ( 1983 ). Comparison of denitrification by Pseudomonas stutzeri, Pseudomonas aeruginosa, and Paracoccus denitrificans . . Appl Environ Microbiol 45, 12471253.[PubMed]
    [Google Scholar]
  2. Clescerl L. S., Greenberg A. E., Eaton A. N. ( 1998 ). Standard Methods for the Examination of Water and Wastewater, , 20th edn.. Washington, DC:: American Public Health Association;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  4. Hirota K., Yamahira K., Nakajima K., Nodasaka Y., Okuyama H., Yumoto I. ( 2011 ). Pseudomonas toyotomiensis sp. nov., a psychrotolerant facultative alkaliphile that utilizes hydrocarbons. . Int J Syst Evol Microbiol 61, 18421848. [View Article] [PubMed]
    [Google Scholar]
  5. Huss V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  6. Jiang H., He X., Zhang L., Tao Y., Wang X., Gao P., Li D. ( 2010 ). [Reduction of selenite to elemental red selenium under aerobic condition by Pseudomonas alcaliphila MBR]. . Wei Sheng Wu Xue Bao 50, 13471352 (in Chinese).[PubMed]
    [Google Scholar]
  7. Kämpfer P., Glaeser S. P. ( 2012 ). Prokaryotic taxonomy in the sequencing era–the polyphasic approach revisited. . Environ Microbiol 14, 291317. [View Article] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  9. Küpfer M., Kuhnert P., Korczak B. M., Peduzzi R., Demarta A. ( 2006 ). Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences. . Int J Syst Evol Microbiol 56, 27432751. [View Article] [PubMed]
    [Google Scholar]
  10. Maiden M. C., Bygraves J. A., Feil E., Morelli G., Russell J. E., Urwin R., Zhang Q., Zhou J., Zurth K. et al. ( 1998 ). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. . Proc Natl Acad Sci U S A 95, 31403145. [View Article] [PubMed]
    [Google Scholar]
  11. Mulet M., Lalucat J., García-Valdés E. ( 2010 ). DNA sequence-based analysis of the Pseudomonas species. . Environ Microbiol 12, 15131530.[PubMed]
    [Google Scholar]
  12. Noel R. K., Penelope J. P. ( 2011 ). Phenotypic and physiological characterization methods. . In Taxonomy of Prokaryotes, Methods in Microbiology, vol. 38, pp. 1560. Edited by Rainey F., Oren A. . London:: Academic Press;. [View Article]
    [Google Scholar]
  13. Palleroni N. J. ( 1993–1994 ). Pseudomonas classification. A new case history in the taxonomy of gram-negative bacteria. . Antonie van Leeuwenhoek 64, 231251. [View Article] [PubMed]
    [Google Scholar]
  14. Palleroni N. J. ( 2003 ). Prokaryote taxonomy of the 20th century and the impact of studies on the genus Pseudomonas: a personal view. . Microbiology 149, 17. [View Article] [PubMed]
    [Google Scholar]
  15. Pascual J., Lucena T., Ruvira M. A., Giordano A., Gambacorta A., Garay E., Arahal D. R., Pujalte M. J., Macián M. C. ( 2012 ). Pseudomonas litoralis sp. nov., isolated from Mediterranean seawater. . Int J Syst Evol Microbiol 62, 438444. [View Article] [PubMed]
    [Google Scholar]
  16. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [View Article] [PubMed]
    [Google Scholar]
  17. Romano I., Nicolaus B., Lama L., Trabasso D., Caracciolo G., Gambacorta A. ( 2001 ). Accumulation of osmoprotectants and lipid pattern modulation in response to growth conditions by Halomonas pantelleriense . . Syst Appl Microbiol 24, 342352. [View Article] [PubMed]
    [Google Scholar]
  18. Saha R., Spröer C., Beck B., Bagley S. ( 2010 ). Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of Pseudomonas oleovorans ATCC 8062T . . Curr Microbiol 60, 294300. [View Article] [PubMed]
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  20. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  21. Su W., Zhang L., Li D., Zhan G., Qian J., Tao Y. ( 2012 ). Dissimilatory nitrate reduction by Pseudomonas alcaliphila with an electrode as the sole electron donor. . Biotechnol Bioeng 109, 29042910. [View Article] [PubMed]
    [Google Scholar]
  22. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 2001 ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51, 16391652. [View Article] [PubMed]
    [Google Scholar]
  23. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  27. Xin Y. H., Zhang D. C., Liu H. C., Zhou H. L., Zhou Y. G. ( 2009 ). Pseudomonas tuomuerensis sp. nov., isolated from a bird’s nest. . Int J Syst Evol Microbiol 59, 139143. [View Article] [PubMed]
    [Google Scholar]
  28. Yamamoto S., Harayama S. ( 1998 ). Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. . Int J Syst Bacteriol 48, 813819. [View Article] [PubMed]
    [Google Scholar]
  29. Yumoto I., Yamazaki K., Hishinuma M., Nodasaka Y., Suemori A., Nakajima K., Inoue N., Kawasaki K. ( 2001 ). Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. . Int J Syst Evol Microbiol 51, 349355.[PubMed]
    [Google Scholar]
  30. Zhan G., Li D., Zhang L. ( 2012 ). Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization. . Appl Microbiol Biotechnol 96, 273281. [View Article] [PubMed]
    [Google Scholar]
  31. Zhang L., He X. H., Zhang L. X., Tao Y., Wang X. M., Zhan G. Q., Li D. P. ( 2011a ). [ Characterization of aerobic reduction of tellurite by Pseudomonas sp. MBR. .] Chin J Appl Environ Biol 17, 126129. (in Chinese). [View Article]
    [Google Scholar]
  32. Zhang T., Zhang L., Su W., Gao P., Li D., He X., Zhang Y. ( 2011b ). The direct electrocatalysis of phenazine-1-carboxylic acid excreted by Pseudomonas alcaliphila under alkaline condition in microbial fuel cells. . Bioresour Technol 102, 70997102. [View Article] [PubMed]
    [Google Scholar]
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