1887

Abstract

Two novel Gram-stain-negative, motile, catalase-negative and oxidase-positive strains of bacteria (JC131 and JC112) were isolated from Lonar, a soda lake in India. Based on 16S rRNA gene sequence similarity studies, strains JC131 and JC112 belong to the family of the class and were most closely related to DQHS21 (98.0 %) and CL-GR15 (96.0 %). Polar lipids of strains JC131 and JC112 include phosphatidylglycerol, phosphatidylethnolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and two unidentified lipids (L1 and L2). Both strains have diplopterol, diploptene, an unidentified hopane (UH) and bacteriohopane derivatives (BHD1 and 2) as major hopanoids and an unidentified pigment (P1). The predominant isoprenoid quinone of both strains was ubiquinone-10 (Q10). Whole-cell fatty acid analysis of both strains revealed that Cω7 was the predominant cellular fatty acid and significant proportions of C, summed feature 3 (Cω7 and/or iso-C 2-OH), 11-methyl Cω7, Cω9, C and Cω7 were also detected. The DNA G+C content of strains JC131 and JC112 was 54.6 and 53.8 mol%, respectively. The genome reassociation (based on DNA–DNA hybridization) of strains JC131 and JC112 with NCCB 100300 ( = DQHS21) was about 58 %, while between JC131 and JC112 it was about 87 %. On the basis of physiological, biochemical and chemotaxonomical properties, strains JC131 and JC112 are differentiated from the other two members of the genus . Strains JC131 and JC112 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC131 ( = KCTC 32038 = NBRC 109022). An emended description of the genus is presented.

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2013-11-01
2024-03-29
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