1887

Abstract

A strictly aerobic, Gram-stain-positive, motile and spore-forming bacterium, strain 3nP4, was isolated from the Puge hot spring located in the south-western geothermal area of China. Strain 3nP4 grew at 38–66 °C (optimum 57–60 °C), at pH 6.0–9.3 (optimum 7.0–7.5) and with 0–4 % (w/v) NaCl (optimum 0–0.5 %). Phylogenetic analysis of 16S rRNA gene sequences, as well as DNA–DNA relatedness values, indicated that the isolate represents a novel species of the genus , related most closely to DSM 12111. Strain 3nP4 had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid as major polar lipids and iso-C and iso-C as major fatty acids, which are both typical chemotaxonomic characteristics of the genus . The mean DNA G+C content of strain 3nP4 was 39.2±0.95 mol% (HPLC). A distinctive characteristic of the novel isolate was its extreme reliance on vitamin mixture or yeast extract for growth. Based on data from this taxonomic study using a polyphasic approach, strain 3nP4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3nP4 ( = CGMCC 1.8979 = JCM 16594).

Funding
This study was supported by the:
  • , Chinese Academy of Sciences , (Award LBB-2011-005)
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2013-11-01
2020-12-04
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