1887

Abstract

A novel ikarugamycin-producing actinomycete, designated strain NEAU-Da3, was isolated from soybean root [ (L.) Merr.] and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-Da3 belonged to the genus , and was most closely related to NRRL B-16359 (99.5 %), VC-A46 (99.3 %) and MCCC 1A01550 (97.2 %); similarities to other type strains of species of the genus were lower than 97.1 %. The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with NRRL B-16359, VC-A46 and MCCC 1A01550. This branching pattern was also supported by the tree reconstructed with the neighbour-joining method. A comparative study between strain NEAU-Da3 and the type strains of the closest related species of the genus revealed that it differed from them in morphological, physiological and biochemical characteristics. Therefore, it is proposed that strain NEAU-Da3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-Da3 ( = CGMCC 4.7047 = DSM 42076).

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2013-10-01
2019-12-07
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