A novel Gram-stain-positive, facultatively anaerobic, non-motile and lactic-acid-producing bacterium, designated strain ORL-24T, was isolated from the gut of the camel cricket, Diestrammena coreana. Optimal growth occurred at 37 °C, pH 8 and with 0 % (w/v) NaCl. The ratio of l-lactate to d-lactate in strain ORL-24T was 96 : 4. Lancefield antigen D was not detected. The strain was negative for oxidase activity and catalase activity. According to a phylogenetic analysis based on 16S rRNA gene sequences, strain ORL-24T was most closely related to the type strain of Enterococcus asini (96.9 % similarity). Comparative pheS and rpoA sequence analyses of strain ORL-24T indicated that the strain belonged to the genus Enterococcus. The major fatty acids were C16 : 0 and C18 : 1ω9c. The DNA G+C content was 41.3 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain ORL-24T represents a novel species of the genus Enterococcus, for which the name Enterococcus diestrammenae is proposed. The type strain is ORL-24T ( = KACC 16708T = JCM 18359T).
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Funding
This study was supported by the:
National Research Foundation of Korea
(Award 2011-0028854)
National Institute of Biological Resources
(Award 2013-02-001)
de VauxA.,
LaguerreG.,
DivièsC.,
PrévostH.(1998).Enterococcus asini sp. nov. isolated from the caecum of donkeys (Equus asinus). . Int J Syst Bacteriol48, 383–387. [View Article][PubMed]
GonzalezJ. M.,
Saiz-JimenezC.(2002). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol4, 770–773. [View Article][PubMed]
KimJ. Y.,
LeeJ.,
ShinN. R.,
YunJ. H.,
WhonT. W.,
KimM. S.,
JungM. J.,
RohS. W.,
HyunD. W.,
BaeJ. W.(2013).Orbus sasakiae sp. nov., a bacterium isolated from the gut of the butterfly Sasakia charonda, and emended description of the genus Orbus
. . Int J Syst Evol Microbiol63, 1766–1770. [View Article][PubMed]
LaneD. J.(1991). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by
StackebrandtE.,
GoodfellowM.
. New York:: Wiley;.
NaserS. M.,
ThompsonF. L.,
HosteB.,
GeversD.,
DawyndtP.,
VancanneytM.,
SwingsJ.(2005a). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology151, 2141–2150. [View Article][PubMed]
NaserS. M.,
VancanneytM.,
De GraefE.,
DevrieseL. A.,
SnauwaertC.,
LefebvreK.,
HosteB.,
SvecP.,
DecostereA.& other authors (2005b).Enterococcus canintestini sp. nov., from faecal samples of healthy dogs. . Int J Syst Evol Microbiol55, 2177–2182. [View Article][PubMed]
RochelleP. A.,
FryJ. C.,
ParkesR. J.,
WeightmanA. J.(1992). DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. . FEMS Microbiol Lett79, 59–65.[PubMed][CrossRef]
SchleiferK. H.,
Kilpper-BalzR.(1984). Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.. Int J Syst Bacteriol34, 31–34. [View Article]
ŠvecP.,
DevrieseL. A.(2009). Genus I. Enterococcus (ex Thiercelin and Jouhaud 1903) Schleifer and Kilpper-Bälz 1984, 32VP
. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 3, p. 594. Edited by
VosP. D.,
GarrityG. M.,
JonesD.,
KriegN. R.,
LudwigW.,
RaineyF. A.,
SchleiferK. H.,
WhitmanW. B.
. New York:: Springer;.