1887

Abstract

Two isolates, with optimum growth temperature and pH of about 30 °C and 6.0–7.0, were recovered from a borehole head of a mineral water aquifer in Portugal. The closest relatives based on 16S rRNA gene sequence analysis were species of genera of the family . Strains L2-4 and L2-109 formed translucent colonies and non-motile pleomorphic cells. Strains were strictly aerobic, and oxidase- and catalase-positive. The major fatty acids of strains L2-4 and L2-109 were 17 : 0 iso 3-OH, 15 : 0 iso and 15 : 1 iso G. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, four unidentified aminophospholipids, four unidentified aminolipids and three unidentified polar lipids. Menaquinone 7 was the only respiratory quinone. The G+C content of the DNA of strains L2-4 and L2-109 was 42.0 and 41.4 mol%, respectively. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics, strains L2-4 ( = CECT 8122 = LMG 26919) and L2-109 ( = CECT 8121 = LMG 26920) are considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is L2-4. Due to additional results obtained in this study an emended description of is provided.

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2013-10-01
2020-01-23
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References

  1. Albuquerque L., Rainey F. A., Nobre M. F., da Costa M. S.. ( 2011;). Schleiferia thermophila gen. nov., sp. nov., a slightly thermophilic bacterium of the phylum ‘Bacteroidetes’ and the proposal of Schleiferiaceae fam. nov.. Int J Syst Evol Microbiol 61:, 2450–2455. [CrossRef][PubMed]
    [Google Scholar]
  2. Albuquerque L., Rainey F. A., Nobre M. F., da Costa M. S.. ( 2012;). Hydrotalea sandarakina sp. nov., isolated from a hot spring runoff, and emended descriptions of the genus Hydrotalea and the species Hydrotalea flava. . Int J Syst Evol Microbiol 62:, 1603–1608. [CrossRef][PubMed]
    [Google Scholar]
  3. An D.-S., Lee H.-G., Im W.-T., Liu Q.-M., Lee S.-T.. ( 2007;). Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57:, 1828–1833. [CrossRef][PubMed]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  5. da Costa M. S., Nobre M. F., Wait R.. ( 2006;). Analysis of lipids from extremophilic bacteria. . In Methods in Microbiology, vol. 35, pp. 127–159. Edited by Rainey F. A., Oren A... Amsterdam:: Elsevier;.
    [Google Scholar]
  6. Degryse E., Glansdorff N., Piérard A.. ( 1978;). A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. . Arch Microbiol 117:, 189–196. [CrossRef][PubMed]
    [Google Scholar]
  7. Hudson J. A., Schofield K. M., Morgan H. W., Daniel R. M.. ( 1989;). Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. . Int J Syst Bacteriol 39:, 485–487. [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Lodders N., Falsen E.. ( 2011;). Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.. Int J Syst Evol Microbiol 61:, 518–523. [CrossRef][PubMed]
    [Google Scholar]
  9. Kim B.-Y., Weon H.-Y., Yoo S.-H., Hong S.-B., Kwon S.-W., Stackebrandt E., Go S.-J.. ( 2007;). Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. . Int J Syst Evol Microbiol 57:, 538–541. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Lim J. H., Baek S.-H., Lee S.-T.. ( 2009;). Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family ‘Chitinophagaceae’ in the phylum Bacteroidetes, isolated from freshwater sediment. . Int J Syst Evol Microbiol 59:, 2394–2399. [CrossRef][PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  13. Nielsen P., Fritze D., Priest F. G.. ( 1995;). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. . Microbiology 141:, 1745–1761. [CrossRef]
    [Google Scholar]
  14. Qu J.-H., Yuan H.-L.. ( 2008;). Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. . Int J Syst Evol Microbiol 58:, 2191–2194. [CrossRef][PubMed]
    [Google Scholar]
  15. Qu J.-H., Yuan H.-L., Yang J.-S., Li H.-F., Chen N.. ( 2009;). Lacibacter cauensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic lake. . Int J Syst Evol Microbiol 59:, 1153–1157. [CrossRef][PubMed]
    [Google Scholar]
  16. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef][PubMed]
    [Google Scholar]
  17. Sangkhobol V., Skerman V. B. D.. ( 1981;). Chitinophaga, a new genus of chitinolytic Myxobacteria. . Int J Syst Bacteriol 31:, 285–293. [CrossRef]
    [Google Scholar]
  18. Shiratori H., Tagami Y., Morishita T., Kamihara Y., Beppu T., Ueda K.. ( 2009;). Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. . Int J Syst Evol Microbiol 59:, 1137–1142. [CrossRef][PubMed]
    [Google Scholar]
  19. Skujins J. J., Potgieter H. J., Alexander M.. ( 1965;). Dissolution of fungal cell walls by a streptomycete chitinase and β-(1-3) glucanase. . Arch Biochem Biophys 111:, 358–364. [CrossRef][PubMed]
    [Google Scholar]
  20. Smibert R. M., Krieg N. R.. ( 1981;). General characterization. . In Manual of Methods for General Bacteriology, pp. 409–443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  22. Tindall B. J.. ( 1989;). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. . FEMS Microbiol Lett 60:, 251–253. [CrossRef]
    [Google Scholar]
  23. Wang Y., Cai F., Tang Y., Dai J., Qi H., Rahman E., Peng F., Fang C.. ( 2011;). Flavitalea populi gen. nov., sp. nov., isolated from soil of a Euphrates poplar (Populus euphratica) forest. . Int J Syst Evol Microbiol 61:, 1554–1560. [CrossRef][PubMed]
    [Google Scholar]
  24. Wiedmann-al-Ahmad M., Tichy H. V., Schon G.. ( 1994;). Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting. . Appl Environ Microbiol 60:, 4066–4071. [CrossRef][PubMed]
    [Google Scholar]
  25. Xie C.-H., Yokota A.. ( 2006;). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56:, 1117–1121. [CrossRef][PubMed]
    [Google Scholar]
  26. Yoon M.-H., Im W.-T.. ( 2007;). Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. . Int J Syst Evol Microbiol 57:, 1834–1839. [CrossRef][PubMed]
    [Google Scholar]
  27. Zhang K., Tang Y., Zhang L., Dai J., Wang Y., Luo X., Liu M., Luo G., Fang C.. ( 2009;). Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. . Int J Syst Evol Microbiol 59:, 3058–3062. [CrossRef][PubMed]
    [Google Scholar]
  28. Zhang K., Wang Y., Tang Y., Dai J., Zhang L., An H., Luo G., Rahman E., Fang C.. ( 2010a;). Niastella populi sp. nov., isolated from soil of Euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella. . Int J Syst Evol Microbiol 60:, 542–545. [CrossRef][PubMed]
    [Google Scholar]
  29. Zhang N. N., Qu J. H., Yuan H. L., Sun Y. M., Yang J. S.. ( 2010b;). Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest. . Int J Syst Evol Microbiol 60:, 1609–1612. [CrossRef][PubMed]
    [Google Scholar]
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