1887

Abstract

Four bacterial strains (JC165, JC166, JC169 and JC170) were isolated from salt pan soils from a coastal region of Tamilnadu, India. They were obligately aerobic, pink- to red-pigmented, mesophilic, haloalkaliphiles having chemoorganoheterotrophic growth on various carbon sources and were catalase- and oxidase-positive. Phototrophic growth and bacteriochlorophyll were absent in all four strains. Major carotenoids present were β-carotene and rhodoxanthin. The main fatty acid in all strains was iso-C. The main polar lipids were phosphatidylethanolamine (PE) and phosphatidylcholine (PC) as well as a few unidentified lipids. Bacterial hopane derivatives and diplopterol (DPL) were detected in all four strains. Based on the 16S rRNA gene sequences, all four strains belong to the family in the phylum . Strains JC165 and JC169 had a sequence similarity of 97.2 % with MIM28, while strains JC166 and JC170 had a sequence similarity of 99.5 % with YIM CH208. Strains JC165/JC169 and JC166/JC170 had genomic DNA reassociation values (based on DNA–DNA hybridization) of 21±2 % and 23±1 % with KCTC 19808 ( = MIM28) and KCTC 22899 ( = YIM CH208), respectively, suggesting that they represented novel species. The reassociation values of >85 % between strains JC165 and JC169, and JC166 and JC170 suggested they were strains of the same species. The genomic information was supported by phenotypic observations leading to the proposal of two novel species, sp. nov. (type strain, JC165 = KCTC 32210 = LMG 27255) and sp. nov. (type strain, JC166 = KCTC 32217 = LMG 27256).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.049924-0
2013-09-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/9/3457.html?itemId=/content/journal/ijsem/10.1099/ijs.0.049924-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Barrow K. D. , Chuck J. A. . ( 1990; ). Determination of hopanoid levels in bacteria using high-performance liquid chromatography. . Anal Biochem 184:, 395–399. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bhadra B. , Roy P. , Chakraborty R. . ( 2005; ). Serratia ureilytica sp. nov., a novel urea-utilizing species. . Int J Syst Evol Microbiol 55:, 2155–2158. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cappuccino J. G. , Sherman N. . ( 1999; ). Microbiology: a Laboratory Manual, , 5th edn.. Menlo Park:: Benjamin/Cummings;.
    [Google Scholar]
  5. Cottrell M. T. , Kirchman D. L. . ( 2000; ). Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. . Appl Environ Microbiol 66:, 5116–5122. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hiraishi A. , Hoshino Y. . ( 1984; ). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. . J Gen Appl Microbiol 30:, 435–448. [CrossRef]
    [Google Scholar]
  7. Hiraishi A. , Hoshino Y. , Kitamura H. . ( 1984; ). Isoprenoid quinone composition in the classification of Rhodospirillaceae . . J Gen Appl Microbiol 30:, 197–210. [CrossRef]
    [Google Scholar]
  8. Imhoff J. F. . ( 1984; ). Quinones of phototrophic purple bacteria. . FEMS Microbiol Lett 25:, 85–89. [CrossRef]
    [Google Scholar]
  9. Kates M. . ( 1972; ). Techniques of Lipidology. New York:: Elsevier;.
    [Google Scholar]
  10. Kates M. . ( 1986; ). Techniques of lipidology: Isolation, Analysis and Identification of lipids. Amsterdam:: Elsevier;.
    [Google Scholar]
  11. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Liu Y. P. , Wang Y. X. , Li Y. X. , Feng F. Y. , Liu H. R. , Wang J. . ( 2012; ). Mongoliicoccus roseus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. . Int J Syst Evol Microbiol 62:, 2206–2212. [CrossRef] [PubMed]
    [Google Scholar]
  14. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  15. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Nedashkovskaya O. I. , Suzuki M. , Vysotskii M. V. , Mikhailov V. V. . ( 2003; ). Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides. . Int J Syst Evol Microbiol 53:, 81–85. [CrossRef] [PubMed]
    [Google Scholar]
  17. Oren A. , Duker S. , Ritter S. . ( 1996; ). The polar lipid composition of Walsby’s square bacterium. . FEMS Microbiol Lett 138:, 135–140. [CrossRef]
    [Google Scholar]
  18. Raj P. S. , Ramaprasad E. V. V. , Vaseef S. , Sasikala Ch. , Ramana Ch. V. . ( 2013; ). Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. . Int J Syst Evol Microbiol 63:, 181–186. [CrossRef] [PubMed]
    [Google Scholar]
  19. Rohmer M. , Bouvier-Nave P. , Ourisson G. . ( 1984; ). Distribution of hopanoid triterpenes in prokaryotes. . J Gen Microbiol 130:, 1137–1150.
    [Google Scholar]
  20. Sasser M. . ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  21. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tian S.-P. , Wang Y.-X. , Hu B. , Zhang X.-X. , Xiao W. , Chen Y. , Lai Y. H. , Wen M. L. , Cui X. L. . ( 2010; ). Litoribacter ruber gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment. . Int J Syst Evol Microbiol 60:, 2996–3001. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tindall B. J. . ( 1990a; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  24. Tindall B. J. . ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  25. Tourova T. P. , Antonov A. S. . ( 1988; ). Identification of microorganisms by rapid DNA-DNA hybridization. . Method Microbiol 19:, 333–355. [CrossRef]
    [Google Scholar]
  26. Trüper H. G. , Pfennig N. . ( 1981; ). Isolation of members of the families Chromatiaceae and Chlorobiaceae . . In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 279–289. Edited by Starr M. P. , Stolp H. , Trüper H. G. , Balows A. , Schlegel H. G. . . Berlin:: Springer;.
    [Google Scholar]
  27. Venkata Ramana V. , Sasikala C. , Veera Venkata Ramaprasad E. , Venkata Ramana C. . ( 2010; ). Description of Ectothiorhodospira salini sp. nov.. J Gen Appl Microbiol 56:, 313–319. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.049924-0
Loading
/content/journal/ijsem/10.1099/ijs.0.049924-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error