1887

Abstract

Four strains (08HL01032, 09HG994, 10HP82-6 and 10HL1960) were isolated from water of air-conditioning systems of various cooling towers in Guangzhou city, China. Cells were Gram-stain-negative coccobacilli without flagella, catalase-positive and oxidase-negative, showing no reduction of nitrate, no hydrolysis of urea and no production of HS. Growth was characteristically enhanced in the presence of -cysteine, which was consistent with the properties of members of the genus . The quinone system was composed of ubiquinone Q-8 with minor amounts of Q-9. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids (PL2, PL3), an unidentified aminophospholipid and an unidentified glycolipid (GL2). The polyamine pattern consisted of the major compounds spermidine, cadaverine and spermine. The major cellular fatty acids were C, C, C, Cω9 and C 3-OH. A draft whole-genome sequence of the proposed type strain 08HL01032 was generated. Comparative sequence analysis of the complete 16S and 23S rRNA genes confirmed affiliation to the genus , with 95 % sequence identity to the closest relatives in the database, the type strains of and subsp. . Full-length deduced amino acid sequences of various housekeeping genes, , , , , , , , , and , exhibited similarities of 67–92 % to strains of other species of the genus . Strains 08HL01032, 09HG994, 10HP82-6 and 10HL1960 exhibited highly similar pan-genome PCR profiles. Both the phenotypic and molecular data support the conclusion that the four strains belong to the genus but exhibit considerable divergence from all recognized species. Therefore, we propose the name sp. nov., with the type strain 08HL01032 ( = CCUG 60119 = NCTC 13503).

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2013-10-01
2019-10-19
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